Yazar "Akbaba, Emel" seçeneğine göre listele

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    Memory-enhancing, Antioxidant, and Anticholinesterase Effects of Inhaled Achillea pseudoaleppica Essential Oil on Scopolamine-induced Amnesic Rats
    (Taylor & Francis Ltd, 2022) Akbaba, Emel; Bagci, Eyup
    Achillea species are used for therapeutic purposes in many countries. This study demonstrated the memory-enhancing, antioxidant, and anticholinesterase effects of Achillea pseudoaleppica essential oil in scopolamine-induced amnesia. The GC/FID-GC/MS system revealed camphor as the major compound in the oil. The application of the essential oil (1% and 3%) was performed on the rats by inhalation for 21 consecutive days before the performance of the behavioral tests (Y-maze and radial-arm maze tests). The essential oil application increased the spontaneous alternation behavior in scopolamine-induced rats in the Y-maze task. Furthermore, essential oil administration decreased working memory errors and reference memory errors in scopolamine-treated rats in the radial-arm maze test. Therefore, Achillea pseudoaleppica essential oil inhalation improved memory in scopolamine-treated rats. In addition, acetylcholinesterase and antioxidant profiles of the hippocampal tissues of the rats were examined. Essential oil administration decreased acetylcholinesterase and superoxide dismutase specific activities and malondialdehyde levels while increasing the total content of reduced glutathione in the rat hippocampus in scopolamine-treated rats. Therefore, in this study, the combination of scopolamine and Achillea pseudoaleppica essential oil improved memory formation and increased the activities of antioxidants and anticholinesterase.
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    Molecular Mechanisms of Melatonin for Treating Medullary Thyroid Cancer Using in silico Analysis
    (Horizon Research Publishing, 2024) Akbaba, Emel
    Melatonin is a neuroendocrine hormone that regulates various biological functions. The objective of this work was to conduct a thorough assessment of the molecular targets of melatonin for the treatment of medullary thyroid carcinoma (MTC) via bioinformatics methods. The identification of target proteins for melatonin and MTC was accomplished through the utilization of many databases. A total of 359 gene targets were acquired, then subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The GO subjects indicated a strong association in kinase activity and receptor-ligand activity between the target genes. On the other hand, the KEGG enrichment analysis indicated a significant involvement of the target genes in the PI3K/AKT and MAPK signaling pathways. To identify key genes, an initial step involved the construction of a protein-protein network using the intersecting targets. Subsequently, Cytoscape software unveiled TP53, STAT3, AKT1, JUN, and IL6 as hub genes within the network. The interaction of melatonin and hub genes was validated by molecular docking analysis. The results indicated that melatonin had high binding affinities towards all of the hub genes, with the exception of JUN. The interaction of melatonin and AKT1 had-10.54 kcal/mol binding free energy with several H-bonds and hydrophobic interactions. Also, the melatonin/p53 complex revealed low binding free energy (-9.95 kcal/mol) with strong H-bond interactions. All the outcomes suggest that melatonin induces apoptosis in MTC cells through the PI3K/AKT and MAPK signaling pathways. Therefore, melatonin has the potential to serve as a powerful therapeutic drug for the treatment of MTC.
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    Neuroprotective effects of Sideritis trojana essential oil in AlCl3-induced amnesic rats
    (Taylor & Francis Ltd, 2024) Hayta, Sukru; Bulmus, Ozgur; Aksoz, Elif; Uysal, Saliha; Akbaba, Emel
    In this study, the effects of Sideritis trojana essential oil on memory, anxiety, and depression were investigated in rats subjected to AlCl3-induced dementia. The major constituents of S. trojana essential oil were analyzed as carvacrol (42.32%), carvone (18.69%), and alpha-pinene (4.54%). Rats were divided into five groups: Control, positive control (donepezil), AlCl3-only applicated, AlCl3 with 40% S. trojana essential oil applicated, and AlCl3 with 80% S. trojana essential oil applicated groups. The essential oil was administered for 30 days, after that, the rats were subjected to behavioral tests. In the forced swimming test, S. trojana essential oil application demonstrated antidepressant-like effects. In the elevated plus maze and open field tests, S. trojana essential oil application exhibited anxiolytic-like effects. Furthermore, the application of S. trojana essential oil ameliorated memory impairment induced by AlCl3 by increasing spontaneous alternation in the Y-maze test. Furthermore, S. trojana essential oil application reduced the level of malondialdehyde and increased catalase activity, demonstrating antioxidant activity. Molecular docking analyses were performed between the major compounds of S. trojana essential oil and the AMPA receptor to evaluate possible mechanisms of action leading to neuroprotection. Carvacrol, carvone, and alpha-pinene were shown to possess strong binding abilities to the AMPA receptor, which may result in allosteric modulation of the receptor. This study demonstrated the neuroprotective effects of S. trojana essential oil in an animal model induced by AlCl3 application, suggesting its potential protective role against central nervous system disorders.
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    Phytochemicals of Hibiscus sabdariffa with Therapeutic Potential against SARS-CoV-2: A Molecular Docking Study
    (2023) Akbaba, Emel; Karatas, Deniz
    In this study, the possible interactions of 17 phytochemicals that were reported as the most abundant biomolecules of Hibiscus sabdariffa, including many organic acids as well as catechin and quercetin derivatives, with 3CLpro and PLpro proteases of SARS-CoV-2 have been investigated via molecular docking. Caffeoylshikimic acid/3CLpro showed the lowest binding energy (-7.72 kcal/mol) with seven H-bonds. The second-lowest binding energy was computed in the chlorogenic acid/3CLpro complex (-7.18 kcal/mol), which was found to form 6 H-bonds. Also, low binding energies of cianidanol (-7.10 kcal/mol), cryptochlorogenic acid (-6.67 kcal/mol), and kaempferol (-6.82 kcal/mol) were calculated to 3CLpro with several H-bond interactions. Nelfinavir (-10.16 kcal/mol) and remdesivir (-6.40 kcal/mol), which have been used against COVID-19, were obtained to have low binding energies to 3CLpro with 3 H-bond formations each. On the other hand, the nicotiflorin/PLpro complex, which had the lowest binding energy (-7.40 kcal/mol), was found to have only 1 H-bond interaction. The second-lowest binding energy was reported in chlorogenic acid/PLpro (-7.20 kcal/mol), which was found to possess four H-bonds. On the other hand, epigallocatechin gallate/PLpro, which was shown to have a -5.95 kcal/mol binding energy, was found to form 8 H-bond interactions. Furthermore, the quercetin pentosylhexoside/PLpro complex was monitored to have low binding energy (-6.54 kcal/mol) with 9 H-bonds, which stands as the highest number of H-bonds in all complexes. Therefore, several molecules of Hibiscus sabdariffa were found to have strong binding affinity to the main proteases of SARS-CoV-2. This study suggests many compounds, including caffeoylshikimic acid and nicotiflorin, to inhibit 3CLpro and PLpro activities. As a result, numerous chemicals derived from Hibiscus sabdariffa have the potential to be employed therapeutically against SARS-CoV-2 infection.