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    INVESTIGATION OF CYTOTOXIC AND ANTI-PROLIFERATIVE EFFECTS OF CRUDE SPIDER VENOM OF Agelena orientalis ON THE BREAST CANCER CELL LINE
    (Parlar Scientific Publications (P S P), 2022) Kayhan, Nazife Yigit; Ocal, Ilkay Corak; Akdeniz, Kader
    Among the venomous animals, spiders have the highest species diversity. In literature, it was reported that spider venoms contain many chemicals including proteins, peptides, polyamine neurotoxins, enzymes, free nucleic acids, monoamines, and inorganic salts. Since spider venoms are rich in molecular content, they can have pharmacologically important molecules. Even though the spiders have important species diversity, only few studies examined the venomous spiders. In these studies, it was shown that spider venoms have cytotoxic, anti-tumoral, antibacterial, antiviral, and insecticidal effects. In the present study, the cytotoxic and anti-proliferative effects of the crude venom of Agelena orientalis C.L. Koch spider on the 1837 breast cancer cell line (MCF-7) and the mouse fibroblast cell line (L929) were examined. In cells treated with venom in cell culture medium, cytotoxic activity was analyzed using 3, [4,5-dimethylthiazol-2-y1]-2,5-diphenylte-trazolium bromide (MTT) test and apoptotic and necrotic cell death was analyzed by using Hoechst 33342 and PI double staining method. Moreover, the cell proliferation was examined by making use of real-time cell analyses system. As a result of the analyses, it was determined that crude spider venom had a cytotoxic effect on MCF-7 and fibroblast cells, and it induced apoptosis in these cells. It was also observed that the crude venom of this spider partially decelerated the proliferation of MCF-7 cells. The present study is the first study reporting the cytotoxic and anti-proliferative effects of A. orientalis spider's crude venom.