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Öğe Investigation of Ivermectin Susceptibility in Kangal and Akbaş Dogs via MDR1 Gene Mutation(2024) Baydan, Emine; Kul, Bengi Çınar; Akkurt, Mustafa Yenal; Yıldırım, Ebru; Arslanbaş, Emre; Aydın, Farah GönülThis research aimed to investigate the sensitivity to the drug (ivermectin) in Kangal and Akbaş breed dogs, which are dog breeds native to Turkey, via the MDR1 gene mutation. For the research, blood, hair and intraoral swap samples were taken from 30 Kangal and 20 Akbaş breed dogs (male-female mixed, adult) with ethical permission and approval forms. Kangal dog samples were taken from the dogs bred in the farms in Sivas center and Kangal village, and Akbaş dog samples were taken from the dogs bred in the farms in the Sivrihisar center, Ankara and Eskişehir regions. The samples taken were evaluated based on polymerase chain reaction (PCR) from the wool and subsequent two-way dideoxy chain termination reaction in the presence of 4 base farm deletions (c.296-299delAGAT) in the 4th exon of the ABCB1 gene that encodes the P-glycoprotein (P-gp) drug transporter protein. According to the obtained DNA sequence results, the deletion of “AGAT” was not determined in any of the individuals screened. The results of this study, which is preliminary research, showed that Kangal and Akbaş breed dogs are safe in terms of sensitivity to drugs that are set to be absorbed and excreted by the P-gp pump, especially ivermectin. However, it would be useful to repeat the analysis of both breeds with more examples.Öğe Seasonal Gene Expression Profile Responsible for Hair Follicle Development in Angora Goats(Kafkas Univ, Veteriner Fakultesi Dergisi, 2023) Kul, Bengi Çınar; Bilgen, Nuket; Biskin, Merve; Akkurt, Mustafa Yenal; Çıldır, Özge Şebnem; Özmen, Özge; Kul, OğuzMammals have physiological reprogramming adaptation ability to changing seasonal light and temperature, through their biological clocks maintained by circadian rhythm, photoperiodism and thermoperiodism. Seasonal differences do not only affect vital activities of animals like migration, reproduction, and sleeping, but also cause dramatic changes in their economically important characteristics (e.g. fur quality and fattening level). Mohair is constituted of non-medullary hairs produced by secondary hair follicles in Angora goats and the effects of seasonal differences on mohair structure and related genes are still unknown. We examined the gene expression levels of BMP-2, FGF-5, HOXC13, KAP9.2 and TGFBR2 normalized with GAPDH in skin biopsies taken from Angora goats (n=20) in two different follicle development stages; telogen (in February) and anagen (in June). HOXC13 showed overexpression in anagen phase (P<0.005) whereas expression was undetectable in telogen phase. BMP-2 (P<0.005), FGF-5 (P<0.005) and TGFBR2 (P<0.01) were significantly upregulated in anagen, while KAP9.2 expression showed no difference between two phases. This is the first study on hair follicle-related genes in the angora goat. Additionally, depending the role of HOXC13 in pathways, it suggests that its overexpression may be one of the main factors associated with the non-medullary hair structure in Angora goats.