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    C-heterochromatin and NORs distribution in karyotypes of three vespertilionid bat species from Turkey
    (Walter De Gruyter Gmbh, 2015) Arslan, Atilla; Zima, Jan; Albayrak, Irfan; Yorulmaz, Tarkan; Arslan, Emine
    The chromosomal banding analysis of the karyotypes of Turkish populations of Eptesicus serotinus, Nyctalus lasiopterus and Barbastellus barbastellus was performed with the use of C-banding and Ag-NOR staining. The results obtained in E. serotinus and N. lasiopterus were congruent with previous data reported from other regions. The karyotype of E. serotinus (2n = 50, NF = 52) contained a moderate amount of centromeric C-heterochromatin and a single NOR was localized in an acrocentric autosomal pairs. The karyotype of N. lasiopterus (2n = 42, NF = 54) contained a higher amount of centromeric C-heterochromatin and the NORs were localized in two autosomal pairs. The karyotype of B. barbastellus was standard in its general characteristics (2n = 32, NF = 54, low amount of C-heterochromatin) but the NOR was localized in only one acrocentric autosomal pair. In studies from other regions, the NORs were recognized in all five acrocentric autosomal pairs of the complement of B. barbastellus.
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    Characterisation of coagulase positive Staphylococcus species isolated from bovine mastitis using protein and plasmid patterns
    (Scientific Technical Research Council Turkey-Tubitak, 2009) Arslan, Emine; Celebi, Ayten; Acik, Leyla; Ucan, Uckun Sait
    The objective of this work was to study the protein patterns, plasmid profiles, and antibiotic susceptibility of Staphylococcus intermedius and Staphylococcus aureus isolates originating from mastitic mammary glands of dairy cattle in different parts of Konya province. A total of 114 Staphylococcus species were isolated and identified by conventional bacteriological methods from bovine mastitis. Of the total isolates 77 were identified as S. aureus and 37 as S. intermedius. Intra- and inter-species diversities in the coagulase-positive staphylococci were investigated by analysis of whole-cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Plasmid profiling also demonstrated that 75 S. aureus isolates and 36 S. intermedius isolates contained plasmid. In addition, 88.3% of S. aureus and 59.4% of S. intermedius isolates were resistant to penicillin. Sixty-six of the 77 S. aureus isolates were also resistant to amoxicillin+clavulanic acid (85.7%). The corresponding number for S. intermedius was 17 (45.9%). Only 1 S. aureus isolate was resistant to danofloxacin. One of each of the Staphylococcus isolates was resistant to methicillin. Results from the study showed that the susceptibility of S. intermedius isolates to antibiotics used widely in mastitis therapy is a matter of concern.