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Öğe The effect of beta-carotene and GnRH administration on luteal size and luteal blood flow after synchronization with prostaglandin F-2 alpha in cows(Wiley-Blackwell, 2010) Ay, Seyhan Serhat; Küçükaslan, İbrahim; Kaya, Duygu; Mulazimoğlu, Serkan Barış; Emre, B.; Kacar, C.; Aslan, S.…Öğe Plasma concentrations of folic acid, vitamin B12 and progesterone of cyclic bitches, bitches during pregnancy and induced abortion and bitches with pyometra(M H Schaper Gmbh Co Kg, 2006) Kalender, Hakan; Beceriklisoy, H. B.; Kanca, Halit; Fındık, Murat; Eruenal-Maral, N.; Handler, J.; Aslan, S.The aim of this study was to investigate the plasma concentrations of folic acid, vitamin B12 and progesterone at different stages of the sexual cycle and pregnancy, during induced abortion and in bitches with pyometra. Bitches (n = 97) were assigned to groups as follows: a) oestrous cycle (n = 42) b) pregnancy (n = 25) c) induction of abortion (n = 10) and d) pyometra (n = 20). Oestrous cycle stages were determined by vaginal inspection and cytology. Pregnancies were estimated by ultrasound (5,0 Mhz; linear transducer; Schimadzu (R)) at days 15-25, 35-45 and 46-63 of pregnancy. Treatments for the induction of abortion were started between days 25 and 35 after mating (5 mu g/kg cabergoline daily, Galastop (R); 5-10 mu g/kg Alfaprostol every other day, Gabbrostim (R)). Diagnosis of pyometra was confirmed by ultrasound and vaginoscopy. Folic acid and vitamin B12 concentrations did not differ among different stages of the oestrous cycle. The mean concentration of folic acid during early pregnancy (days 15-25) exceeded levels of later stages (days 46-63): 9,4 +/- 3,7 mu g/ml and 4,7 +/- 1,8 mu g/ml, respectively (p < 0,01). A positive correlation between folic acid and vitamin B12 was determined in pregnant dogs (r = 0,925; p < 0,02). Before the induction of abortion, the concentration of folic acid was 9,6 +/- 5,2 mu g/ml; during abortion it decreased to 5,0 3,2 mu g/ml (p < 0,01). A significant correlation (r = 0,925; p < 0,02) between progesterone and folic acid was obtained in bitches with abortion. The mean concentration of folic acid in bitches with pyometra significantly differed from that of bitches at different stages of the oestrous cycle (p < 0,05). The mean concentration of folic acid was significantly lower in metoestrous bitches when compared to bitches with pyometra (p < 0,05). The decrease of serum concentrations of folic acid during pregnancy and induced abortion show that fetal growth and abortion caused higher consumption of folic acid. Concerning bitches did not show any deficiency symptoms which is why it can be concluded that this decrease is physiological.Öğe The relationship between the stages of the sexual cycle, the pregnancy and postpartum periods and vaginal flora in Kangal Breed bitches(Scientific Technical Research Council Turkey, 2003) Findik, M.; Maral, N.; Keskin, O.; Kalender, H.; Erdeger, J.; Aslan, S.This study was performed on females of the Kangal Breed (Turkish Anatolian Shepherd Dog) with the aim of determining the relationship between the stages of the sexual cycle and vaginal flora. The stages of the cycle in Kangal bitches (n = 16) were determined by performing vaginoscopic, vaginal cytologic and ultrasonographic checks at regular intervals and examining vaginal swab samples bacteriologically. The swab samples were inoculated onto 7% sheep blood agar and incubated for 18-24 h at 37 degreesC and total counts of the resulting colonies were evaluated. In addition, inoculations were made onto 7% sheep blood agar, PPLO agar, Columbia agar and Brucella agar with serum and incubated in a 10% CO2 environment at 37degreesC and onto Saboraud dextrose agar, and these were incubated at room temperature and the resulting colonies identified. In all stages of to cycle E. coli and also Streptococci, were isolated, except in the pregnancy period. It was determined that haemolytic streptococci grew at the estrus and early metestrus stages while alpha-haemolytic streptococci grew at other stages of the cycle. Staphylococci were not found at the pre-proestrus and estrus stages, whereas they were isolated in various percentages from samples taken at the other stages. At the stages following the end of estrus, Citrobacter, Flavobacter and Proteus spp. were isolated in different percentages. Enterobacter, Acinetobacter and Neisseria spp. were isolated at different stages of estrus, Pasteurella spp. at late estrus and Shigella spp. as well as yeast in the pre-proestrus periods. While the E coli was grown at a rate of 76.9% from the samples from breeding bitches, the coincidence rates of B-haemolytic streptococci, alpha-haemolytic streptococci as well as S. aureus were determined to be 15.4%, 15.4% and 38.5%, respectively. Pasteurella, Neisseria and S. epidermidis levels were the same; 7.7%. It was observed that, E. coli appeared at a level of 100% in all the non-pregnant bitches, and E. coli alone was isolated at a level of 75%. Similarly E. coli appeared in six of the bitches that became pregnant (66.7%) and E. coli was isolated alone from half of these samples (50%). As a result it was observed that some of the bacterial components comprising the vaginal flora were present at every stage of the sexual cycle, while some were absent at particular stages; bacteria could grow at the estrus stage in the vagina without influencing the outcome of the pregnancy.Öğe A screening for the occurrence of autoreactive antibodies in sera of pregnant and non-pregnant bitches(Wiley, 2006) Schafer-Somi, S.; Herkner, K. R.; Neubauer, S.; Egerbacher, M.; Patzl, M.; Pollak, S; Aslan, S.Sera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit((R)). In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T-3 and T-4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1 : 100) for T-3-AAb was detected.Öğe Vascular Endothelial (VEGF) and Epidermal Growth Factor (EGF) as well as Platelet Activating Factor (PAF) and receptors are expressed in the early pregnant canine uterus(Wiley-Blackwell, 2012) Schaefer-Somi, S.; Sabitzer, S.; Klein, D.; Reinbacher, E.; Kanca, H.; Beceriklisoy, H. B.; Aslan, S.…Öğe Vascular Endothelial (VEGF) and Epithelial Growth Factor (EGF) as Well as Platelet-Activating Factor (PAF) and Receptors are Expressed in the Early Pregnant Canine Uterus(Wiley-Blackwell, 2013) Schaefer-Somi, S.; Sabitzer, S.; Klein, D.; Reinbacher, E.; Kanca, H.; Beceriklisoy, H. B.; Aslan, S.Contents The aim of this study was to investigate the course of expression of platelet-activating factor (PAF), PAF-receptor (PAF-R), epidermal growth factor (EGF), EGF-R, vascular endothelial growth factor (VEGF), VEGF-R1 and VEGF-R2 in uterine tissue during canine pregnancy. For this purpose, 20 bitches were ovariohysterectomized at days 1012 (n = 10), 1825 (n = 5) and 2845 (n = 5) days after mating, respectively. The pre-implantation group was proven pregnant by embryo flushing of the uterus after the operation, the others by sonography. Five embryo negative, that is, non-pregnant, bitches in diestrus (day 1012) served as controls. Tissue samples from the uterus (placentation sites and horn width, respectively) were excised and snap-frozen in liquid nitrogen after embedding in Tissue Tec (R). Extraction of mRNA for RT-PCR was performed with Tri-Reagent. In the embryos, mRNA from all factors except VEGF was detected. In the course of pregnancy, significantly higher expression of PAF and PAFR as well as VEGF and VEGFR2 during the pre-implantation stage than in all other stages and a strong upregulation of EGF during implantation were characteristic. The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy.
