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    Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen
    (Walter De Gruyter Gmbh, 2018) Sahin, Aydan Acar; Aslim, Belma; Tan, Sema; Alan, Senol; Pinar, Nur Munevver
    Objective: Cupressaceae pollen has commonly been reported to be an important aeroallergen and causal factor of spring, autumn and winter pollinosis in many countries. The aim of this study was to compare of the structure and allergenic protein content of Cupressus arizonica Greene., Cupressus sempervirens L. and Juniperus oxycedrus L. pollen in detail and contribute to Cupressaceae pollen allergen diagnosis and therapy studies in Turkey. Methods: The pollen structure were examined by LM and SEM. Pollen protein content was investigated by Bradford protein assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot analysis and two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), respectively. Pectate lyase (PL) enzyme activities were compared. Immunoblotting was carried out by using extracts of the three taxa pollen collected from Turkey. Results: All three taxa was found very similar in terms of pollen morphology however, intine thickness was prominently different. Cupressus arizonica pollen extracts showed the lowest PL activity. Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions: As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to improve the Cupressaceae pollen allergen diagnosis and therapy.
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    Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition
    (Elsevier Sci Ltd, 2014) Ozturk, Sahlan; Aslim, Belma; Suludere, Zekiye; Tan, Sema
    In the present study, chromium, cadmium and metal mixed (chromium + cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp. BAS0671 were investigated. It was investigated that BAS0671 showed different removal ability when exposed to each metal solely and mixed metal. EPS production by BAS0671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI) + Cd(II). Monomer composition of EPS was changed after metal treatment. Uronic acid contents of metal treated cells were higher than control cells of each isolate. Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells. Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface. Fourier transform infrared spectrum analysis of EPSs indicated the presence of C-H and C-O group, which may serve as binding sites for divalent cations. (C) 2013 Elsevier Ltd. All rights reserved.
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    Removal and reduction of chromium by Pseudomonas spp. and their correlation to rhamnolipid production
    (Elsevier Science Bv, 2012) Ozturk, Sahlan; Kaya, Tayfun; Aslim, Belma; Tan, Sema
    Chromium removal and its association with rhamnolipid production in Pseudomonas spp. were investigated. Three Pseudomonas spp. isolates (P. aeruginosa 78, P. aeruginosa 99, and P. stutzeri T3) were investigated with regard to their exposure to 10 mg/L for chromium removal. P. aeruginosa 99 removed 16% and 20% more chromium than P. stutzeri T3 and P. aeruginosa 78 respectively. The reduction of Cr(VI) to Cr(III) by all the three isolates is more or less similar. P. aeruginosa 99, which removed higher chromium, also produced higher rhamnolipid (165 +/- 5 mg/mL). P. aeruginosa 78, which removed lower chromium, also produced lower rhamnolipid (126 +/- 3 mg/mL). Rhamnolipid production by P. aeruginosa 78 and P. aeruginosa 99 was increased in its exposure to 10 mg/L chromium. In the present study, results showed that rhamnolipid might play a role in chromium removal by three Pseudomonas spp. isolates. (C) 2012 Elsevier B.V. All rights reserved.