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    Investigation of Theileria annulata and Theileria buffeli/orientalis in Cattle from Kirikkale - Turkey by Reverse Line Blotting Analyses
    (Israel Veterinary Medical Assoc, 2016) Gokpinar, S.; Aydenizoz, M.
    This study was aimed at determining the prevalence of T. annulata and T. buffeli/orientalis species in cattle from Kirikkale province using reverse line blotting (RLB) and comparing the results with microscopy. Blood samples were collected from 294 cattle from 9 districts of Kirikkale (Centre, Bahsili, Baliseyh, Celebi, Delice, Karakecili, Keskin, Sulakyurt and Yahsihan) between May-October 2010. From cattle that has at least grazed once and over one year old of age. A blood smear from ear tip peripheral blood was prepared and a venous blood sample from jugular vein was taken. Blood smears were inspected and documented for the presence of Theileria spp. piroplasms after Giemsa staining. Venous blood samples were used for extraction of DNA for Reverse Line Blotting (RLB) analyses. Out of 294 samples analyzed 44 (15%) were found to have Theileria spp. piroplasms with microscopic examination, while 77 (26.1%) harbored T. annulata as determined by RLB. In none of the samples T. buffeli/orientalis was detected. The prevalence of Theileria spp. and T. annulata was highest in Keskin district. This study presents the first investigation of Theileria species in cattle from Kirikkale province.
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    Prevalence of anoplocephalidae species in sheep and cattle slaughtered in Kirikkale, Turkey
    (Ecole Nationale Veterinaire Toulouse, 2003) Aydenizoz, M.; Yiıdız, K.
    This study was undertaken to determine the prevalence of Anoplocephalidae infections in sheep and cattle slaughtered in Kirikkale. In this study, small intestines of 3 133 sheep and 870 cattle were examined. Infection was determined in 4.43% and 0.34% of sheep and cattle examined, respectively. The species of Anoplocephalidae found were Moniezia expansa (3.98%), Avitellina centripunctata (0.86%) and Thysaniezia ovilla (0.15%) in sheep and M. benedeni (0.22%) and M. expansa (0.11%) in cattle. The species determined were M. expansa 89.92%, A. centripunctata 19.42%, T. ovilla 3.59% in infected sheep and M. benedeni 66.66%, M. expansa 33.33% in infected cattle. The mixed infections observed were 87.76% (with one species), 11.51% (with two species) and 0.71% (with three species) in sheep, and cattle infected only with one species was 100%. The number of scolex observed was 1-33 and 1-3 in infected sheep and cattle, respectively. The number of mean scolex was 4.72 and 2.33 in sheep and cattle, respectively. The infection observed was the highest in July (9.89%) and the lowest in September (1.32%) in sheep. However, in cattle the highest and lowest infection observed was in October (1.51%) and September (1.11%),respectively. Infection rate observed was higher in sheep (4.43%) than that of cattle (0.34%). This observation was also statistically significant (p < 0.01).