Yazar "Ayva, Şebnem" seçeneğine göre listele
Listeleniyor 1 - 8 / 8
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A rare synchronous tumor combination of primary squamous cell lung cancer and urinary papillary cell carcinoma(AVES, 2011) Kavut, Ayşe Baççio?lu; Kalpaklio?lu, Füsun; Ayva, Şebnem; Batislam, ErtanThe incidence of primary bladder and lung cancer occurring together is rare. Here, a case of a man who was diagnosed with end stage lung cancer and early stage bladder cancer was discussed with the interesting aspects. Despite the fact that the patient had a cough, weight loss and dyspnea which are the most common symptoms of lung cancer, hematuria was the main complaint that induced the patient to consult the doctor. The case is valuable for the review of the literature about multiple primary malignancies of squamous cell lung carcinoma and urinary bladder transitional cell carcinoma. It is also important for reminding clinicians to keep in mind that smoking is the most important common predisposing factor for both lung and bladder carcinoma in this case.Öğe Autologous Blood Pleurodesis in Rats to Elucidate the Amounts of Blood Required for Reliable and Reproducible Results(Academic Press Inc Elsevier Science, 2010) Özpolat, Berkant; Gazyağcı, Serkal; Gözübüyük, Alper; Ayva, Şebnem; Atinkaya, CanselBackground. Pleurodesis is used in the treatment of spontaneous pneumothorax or refractory pleural effusions of different etiologies. Several agents have been employed, but many questions remain unanswered about their effectiveness and toxicity. Use of autologous blood pleurodesis in clinical practice has been described in the literature without any clear consensus regarding its efficacy. Experimental studies using this technique are limited to a single study in rabbits. We performed a prospective, randomized, observer-blinded, controlled study to evaluate the safety and efficacy of increasing doses of autologous blood pleurodesis in a novel rat model. Materials and Methods. Twenty-eight albino Wistar rats were divided into four groups. Groups 1, 2, and 3 were the study groups and group 4 was the control group, with seven animals in each group. Groups 1, 2, and 3 were given autologous blood, 1mL/kg, 2mL/kg, 3mL/kg, respectively, and group 4 (control) was given only 2mL/kg saline intrapleurally. The rats were sacrificed on postoperative day 30. The surfaces were graded by macroscopic (visible adhesion formation) and microscopic (inflammation and fibrosis) examination. Results. Macroscopically, group 2 and group 3 developed significantly more adhesions; 3mL/kg autologous blood produced the most significant pleurodesis with generalized adhesions seen between visceral, parietal, and mediastinal pleura. Microscopic examination showed that all study groups developed an inflammatory response at the site of blood injection. There were no pathologic changes in ipsilateral and contralateral lung parenchyma. Conclusions. Autologous blood at doses 2-3mL/kg were shown to be effective to produce adhesions in 30 d, and the results were highly reproducible in all rats. We propose that the occasional negative results obtained in humans may be related to an insufficient amount of injected blood, as observed in our rat model. (C) 2010 Elsevier Inc. All rights reserved.Öğe Dermal vacuoles in two biopsies of psoriasis(Wiley-Blackwell, 2008) Ayva, Şebnem; Erkek, Emel; Atasoy, PınarTwo patients presented with cutaneous lesions clinically typical of psoriasis. The first case was a 38-year-old man and the second was a 51-year-old woman. To confirm the diagnosis, 4-mm punch biopsy samples were obtained from both patients from the lesions on the knees. Histology in both cases was in favour of psoriasis and also revealed empty vacuoles in the papillary dermis, concentrated at sites of intense lymphocyte infiltration. The empty vacuoles resembled true fat cells or fat globules. They did not reveal positive immunostaining with CD34 antigen, suggesting that they were not lined by endothelial cells. Final histological diagnosis was psoriasis associated with dermal vacuolization.Öğe Effect of Penile Tourniquet on Growth Factors in Rat Penile Tissue(Georg Thieme Verlag Kg, 2009) Kaya, Murat; Soyer, Tutku; Ayva, Şebnem; Çakmak, MuratAim: This study aimed to evaluate the effect of penile tourniquet application on growth factors in rat penile tissues. Materials and Methods: Forty Wistar male rats were included in the study. Rats were divided into 4 groups. After anesthetization, perimeatal penile skin and the corpus cavernosum were sampled in the control group (CG). A Mathieu-like flap was designed without a penile tourniquet (PT) to serve as a sham group (SG). In the PT groups, a Mathieu-like flap was created and a 5 mm diameter rubber circular band was applied at the base of the penis. The PT was applied for 10 min in the PT-10 group and for 30 min in the PT-30 group. Penile tissue was sampled 24 h after PT application in the SG and PT groups. Tissues obtained were examined in three sections: the subepithelial vascular plexus (SVP), the corpus cavernosum (CC) and the smooth muscle-like mesenchymal cells in the corpus cavernosum (MC). Acute inflammation was evaluated with hematoxylin-eosin staining. The effect of PT on vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR) and transforming growth factor 0 receptor (TGF beta-R) levels was evaluated. Results: Higher grades of acute inflammation were encountered in the PT-10 and PT-30 groups compared to the CG and SG (p<0.005). However, mean grades of acute inflammation did not show a statistical difference between the PT-10 and PT-30 groups (p>0.05). When the levels of growth factors were compared between the CG and PT-10 group, the PT-10 group showed increased levels of VEGF and TGF beta-R. In the PT-30 group, both VEGF and VEGFR levels were found to be decreased. When acute inflammation grades of tissues were correlated with VEGF and TGF beta-R, higher acute inflammation grades correlated with decreased VEGF and increased TGF beta-R levels (Spearman's correlation, p<0.005). Although alterations in VEGF and TGF beta-R levels were detected in the SVP and CC of penile tissues, altered VEGFR levels were only detected in the MC sections. Conclusion: PT caused higher grades of acute inflammation which correlated with decreased VEGF levels and increased TGF beta-R levels. Decreased VEGF levels after PT may alter the angiogenesis phase of wound healing and cause poor angiogenesis in penile skin flaps. Increased levels of TGF beta-R can be considered as an acute inflammatory response to PT. These results confirmed that prolonged PT application may result in altered growth factors in penile tissue and may reduce the success rate of repair.Öğe Expression profile and prognostic importance in prostate lesions of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57kip2a)(Springer, 2009) Atasoy, Pınar; Yılmaz, Erdal; Bozdoğan, Önder; Ayva, Şebnem; Batislam, ErtanTo investigate expression of the reverse transcriptase component of human telomerase (hTERT) and of cyclin-dependent kinase inhibitor p57 (p57(kip2a)) in prostate neoplasms and evaluate the relationship between these proteins and the Gleason score. hTERT and p57(kip2a) antibodies were studied by immunohistochemical methods in 70 prostate adenocarcinomas (33 high-grade and 37 low-grade carcinomas), 29 benign prostate hyperplasias, and 19 prostatic intraepithelial neoplasias (PIN). Only nuclear staining was evaluated with p57(kip2a) whereas both nuclear and nucleolar staining were evaluated with hTERT. Immunohistochemical histologic scores (HSCOREs) of hTERT were significantly higher in the PIN group than in the hyperplasia group (P = 0.03). hTERT HSCOREs were not significantly different between hyperplasias and carcinomas or between low and high-grade carcinomas. p57(kip2a) HSCOREs were significantly higher in hyperplasias than other groups, and in PINS than carcinomas, but did not differ significantly between low and high-grade carcinomas. A significant negative correlation was observed between hTERT and p57(kip2a) (P = 0.007) in the hyperplasia group. No such correlation was found in PINs and carcinomas. This study suggests that p57(kip2a) is down-regulated in the malignant side of the spectrum of prostate carcinogenesis. Loss of p57(kip2a) control on hTERT might have an important role in the development of prostate cancer.Öğe İnvajinasyonda oluşan iskemi/reperfüzyon hasarına trapidilin koruyucu etkileri(2009) Çakmak, Murat; Soyer, Tutku; Ayva, Şebnem; Dikmen, Günnur; Akman, HülyaAmaç: Platelet-derivated-growth faktör inhibitörünün (Trapidil) invajinasyona bağlı iskemi reperfüzyon hasarını önleyici etkinliğini değerlendirmek üzere deneysel bir ça- lışma yapılmıştır. Gereç ve Yöntem: Ağrlıkları 200-250 g olan, her iki cinste 60 Wistar albino rat çalışmaya dahil edildi. Kontrol gru- bunda (KG, n:15), ileoçekal valvin 20 cm proksimaldeki 2 cm’lik barsak segmenti, intraperitoneal NaCl infüzyonunu takiben çıkartıldı. İnvajinasyon grubunda, invajinasyon modeli (IG, n:15) ileoçekal valvin 20 cm proksimalindeki barsağın distaldeki barsağa doğru stile yardımıyla itilmesi ile elde edildi. İnvajinasyon 4 saat sonra ileoçekal valvden 20 cm proksimaldeki 2 cm’lik barsak segmenti çıkartıldı. İnvajinasyon-reperfüzyon grubu (IRG, n:15) IG’ye benzer şekilde invajinasyon oluşturulduktan 4 saat sonra reperfüz- yona izin verilerek elde edildi. Bu grupta barsak örneklen- mesi reperfüzyondan 4 saat sonra yapıldı. Son grupta (TG, n:15), Trapidil (40 mg/kg) İRG’a benzer şekilde iskemi/re- perfüzyon oluşturulduktan ve reperfüzyondan bir saat önce intraperitoneal olarak verildi. Bu grupta da barsak segmen- ti reperfüzyondan 4 saat sonra çıkarıldı. Gruplarda ileoçe- kal valvden 20 cm proksimalden 2 cm’lık barsak örnekleri histopatolojik değerlendirme için alındı. Örnekler ayrıca oksidatif hasar belirteçleri (katalaz, glutatyon peroksidazG-px, superoksit dismutaz-SOD ve malondialdehit-MDA) açısından da değerlendirilmiştir. Bulgular: Histopatolojik değerlendirmelerde KG’de ha- sara rastlanmazken, diğer gruplarda yüksek evreli barsak hasarı izlenmiştir (p<0.05). Ancak IG, IRG ve TG arasında histopatolojik evre bakımından anlamlı fark bulunmamak- tadır. MDA seviyeleri deney gruplarında KG’e göre belir- gin yüksektir. Buna karşın TG’de MDA seviyeleri, IRG ile karşılaştırıldığında belirgin azalmıştır (p<0.05). Antioksi- dan enzimler; katalaz, G-px ve SOD, IG ve IRG’de anlamlı derecede azalmıştır. IRG ile karşılaştırıldığında, Trapi- dil verilmesi bu üç enzim düzeyini arttırmıştır (p=0.005, p=0.036, p=.0.02 sırasıyla). Sonuç: Trapidil invajinasyona bağlı iskemi reperfüzyon hasarını katalaz, G-px ve SOD enzim düzeylerini arttırarak önlemekte ve MDA düzeylerinde anlamlı azalmaya neden olmaktadır. Fakat benzer sonuçlara histopatolojik değer- lendirmelerde ulaşılmamıştır.Öğe Protective effect of intraperitoneal ozone application in experimental ovarian ischemia/reperfusion injury(W B Saunders Co-Elsevier Inc, 2012) Aslan, Mustafa Kemal; Boybeyi, Özlem; Şenyücel, Mine F.; Ayva, Şebnem; Kısa, Üçler; Aksoy, Nurkan; Çakmak, MuratAim: The current accepted management of ovarian torsion is ovary-sparing surgery. Ozone therapy is used to reduce ischemia/reperfusion (I/R) injury in several situations. An experimental study was designed to evaluate effect of ozone application in ovarian I/R injury. Materials and methods: Three groups (n = 6) and 18 rats were included in the study. After anesthesia, right ovaries were fixed and removed at the end of 2 hours in sham group (SG). In torsion group (TG), right ovaries underwent 720 torsion in a counterclockwise direction. Ovaries were removed after 2 hours torsion and 2 hours reperfusion. In ozone group (OG), torsion was created by the same technique, and 95% oxygen plus 5% ozone gas mixture was given intraperitoneally (25 mu g/mL, 0.5 mg/kg) 10 minutes before reperfusion. After 2 hours reperfusion, ovaries were removed. Histopathologic examination of ovarian and periovarian sections was performed for the presence of congestion (C), hemorrhage, interstitial edema (IE), and polymorphonuclear neutrophilic infiltrations. Tissue samples were analyzed for malondialdehyde, nitric oxide (NO), and total sulphidryl (t-SH) values. Results were compared between 3 groups. Results: At histopathologic examination, the TG have elevation in terms of ovarian C, polymorphonuclear neutrophilic infiltration, and periovarian IE when compared with SG (P < ,05). In OG, ovarian C and periovarian IE were reduced according to TG, whereas the increase was observed only in ovarian C compared with SG (P < .05). At biochemical evaluation of oxidative stress markers in SG and TG, there was no difference between them (P < .05). Malondialdehyde levels were significantly lower in OG than TG, whereas NO and t-SH values were higher (P < .05). Malondialdehyde levels were decreased in OG compared with SG (P < .05). However, no difference was observed in NO and t-SH levels (P > .05). Conclusion: Intraperitoneal application of ozone creates a positive impact on histologic and biochemical markers on I/R injury owing to ovarian torsion. The ozone application can be developed to support efforts to protect ovary in ovarian torsion. (C) 2012 Elsevier Inc. All rights reserved.Öğe The protective effect of trapidil on ischemia/reperfusion injury in intussusceptions(2009) Çakmak, Murat; Soyer, Tutku; Ayva, Şebnem; Dikmen, G.; Akman, H.Aim: An experimental study was performed to evaluate the protective effect of platelet-derivated-growth factor inhibitor (Trapidil) in ischemia reperfusion injury in intussusception. Methods: Sixty Wistar albino rats, weighing 200-250 g in both sexes were enrolled into study. In control group (CG, n:15), 2 cm of intestinal segment, 20 cm proximal to ileoceacal valve was removed after intraperitoneal NaCl infusion. In intussusception group, intussusception (IG, n:15) was performed at 20 cm proximal to ileoceacal valve by pushing the proximal intestinal segment distally through a stile. Two cm of intestinal segment was removed 4 hours after intussusception. Intussusception-reperfusion group (IRG, n:15) was obtained after performing intussusception similar to IG and allowed to perfusion 4 hours after intussusception. In this group, intestinal segment was removed 4 hours after reperfusion. In the last group (TG, n:15), Trapidil (40 mg/kg) was administered intraperitoneally, one hour before reperfusion, after achiving an ischemia/reperfusion injury similiar to IRG. Two cm of intestinal segments, 20 cm proximal to ileoceacal valve, were removed for histopathologic evaluation. The specimens were also evaluated for oxidative injury markers (catalase, glutathione peroxidase-G-px, superoxide dismutase-SOD, and malondialdehyde-MDA). Results: Although histopathologic evaluations revealed no intestinal injury in CG, other groups showed higher grades of intestinal injury (p<0.05). Though, there was no difference in histopathologic grades between IG, IRG and TG groups. MDA levels were significantly increased in experiment groups when compared to CG. But, significant alteration in MDA levels was detected in TG, when compared to IRG (p=0.00). Antioxidant enzymes; Catalase, G-px and SOD, significantly decreased in IG and IRG. Trapidil administration increased the levels of these three enzymes when compared to IRG (p=0.005, p=0.036, p=.0.02 respectively). Conclusion: Trapidil prevents ischemia-reperfusion injury in intussusception by increasing catalase, G-px, SOD levels and causes a significant reduction in MDA levels. However, similar results was not found in histopathologic findings.
