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Öğe Can Thiol/Disulphide Homeostasis Help in The Differential Diagnosis of Appendicitis in Children?(2020) Demir, Sabri; Günal, Yasemin Dere; Özmen, İsmail; Badem, Nermin Dindar; Neselioglu, Salim; Erel, ÖzcanObjective: We aimed to investigate the potential of assessing thiol/disulfide homeostasis as novel oxidative stressmarkers to improve the challenging diagnosis of acute appendicitis in children.Material and Methods: A total of 60 pediatric patients (0-18 years) were enrolled in the study, 30 of which were inthe control group and 30 in the acute appendicitis group. Native thiol, total thiol, dynamic disulfide, dynamic thiol,ischemia modified albümin, albumin, White blood cell, hemoglobin, hematocrit, and platelet counts of both groups weremeasured. The results of both groups were compared using the SPSS (Statistical Package for Social Sciences) version17 (Chicago, USA) program. For all variables, p <0.05 was considered significant.Results: Total thiol (p<0.001), native thiol (p<0.001), and albümin (p<0.001) levels were significantly decreased whiledynamic disulfide, dynamic thiol (p=0.003), and ischemia modified albümin (p<0.001) levels those indicating oxidantside were increased in acute appendicitis group compared to control. White blood cell counts in acute appendicitisgroup were higher (p<0.001), and platelet counts were lower (p=0.03) than the control group.Conclusion: In the differential diagnosis of acute appendicitis, especially in case that are difficult to diagnose, besides aphysical examination, imaging, and current laboratory tests, quantification of thiol/disulfide homeostasis may be helpfulin diagnosing. In addition, evaluating albumin and IMA levels may increase the specificity of the test. This test can bemore helpful in cases diagnosis is difficult such as children small in their ages and mental retardation.Öğe Could erythropoietin reduce the ovarian damage of cisplatin in female rats?(Taylor & Francis Ltd, 2018) Sayan, Cemile Dayangan; Tulmac, Ozlem Banu; Karaca, Gokhan; Ozkan, Zehra Sema; Yalcin, Selim; Devrim, Tuba; Badem, Nermin DindarThe aim of this study is to investigate whether erythropoietin (EPO) can reduce the ovarian damage of cisplatin or not. Thirty, female, Wistar-Albino rats were used in the study. Control group (N=10): Intraperitoneal saline infusion, Cisplatin group (N=10): Intraperitoneal 7mg/kg cisplatin, Cisplatin+EPO group (N=10): Intraperitoneal 7mg/kg cisplatin and subcutaneous 200IU/kg/day EPO. Serum AMH concentrations were measured by enzyme-linked immunosorbent assay kit of AMH. Follicular counts were evaluated according to mean diameter of the follicles. Ovarian damage; including follicular cell degeneration, vascular congestion, hemorrhage, and inflammation was scored histologically using a graduated scale. Posttreatment AMH levels of cisplatin group were significantly lower than control and cisplatin+EPO groups. In cisplatin group, there was a significant decrement in posttreatment AMH level compared to pretreatment AMH level. The total damage score of cisplatin group was significantly higher than scores of control and cisplatin+EPO groups. The mean primordial follicle counts of control and cisplatin+EPO groups were significantly higher than that of cisplatin group (p=.007 and p=.003). The results of this study revealed that EPO administration to cisplatin chemotherapy could ameliorate the ovarian damage. Erythropoietin administration to chemotherapeutic agents might suggest to protect ovarian failure and infertility.Öğe Diagnostic Value of Plasma Soluble Triggering Receptor Expressed on Myeloid Cells-1 in Children with Urinary Tract Infections(Georg Thieme Verlag Kg, 2021) Tursun, Serkan; Alpcan, Aysegul; Ozsoy, Metin; Badem, Nermin Dindar; Kandur, Yasar; Acar, Banu CelikelObjective The aim of the present study was to evaluate the diagnostic value of soluble triggering receptor on myeloid cells-1 as a novel marker for diagnosis of childhood urinary tract infections (UTI). Methods This study enrolled 30 pediatric patients diagnosed with acute febrile UTIs; 30 healthy children were included as the control group. The blood samples from the patients and healthy controls were collected for a soluble triggering receptor on myeloid cells-1 (sTREM-1) test. Results The study group was composed of 9 males and 21 females, and the mean age of the study population was 6.63.2 (range=1-14) years. sTREM-1 levels were significantly higher in UTI patients than in the controls (592 +/- 323 vs. 490 +/- 299 pg/mL, p=0.04). The receiver operating curve analysis revealed a cut-off value of soluble triggering receptor expressed on myeloid cells-1 of 514 ng/mL (area under the curve=0.562). When the cut-off value was taken 514 pg/mL, soluble triggering receptor expressed on myeloid cells-1 had a sensitivity of 57% and a specificity of 50% for the diagnosis of UTI. Conclusion The present study revealed that plasma sTREM-1 level may be elevated in UTI and may therefore serve as a useful predictive tool for the diagnosis of UTI.Öğe The Diagnostic Value of sTWEAK in Acute Ischemic Stroke(GALENOS YAYINCILIK, 2020) Comertpay, Ertan; Vural, Sevilay; Eroglu, Oguz; Badem, Nermin Dindar; Bilgili, Yasemin Karadeniz; Coskun, FigenBackground: Considering the critical role of early diagnosis and management of acute ischemic stroke, biomarkers that can reliable assist in the diagnosis are still needed. These biomarkers should rapidly analyze, have high specificity for brain damage, and be available in the emergency settings for early diagnosis and exclusion of other conditions that mimic acute ischemic stroke. Soluble tumor necrosis factor-like weak inducer of apoptosis, a protein involved in the regulation of several biological functions, could be a potential acute ischemic stroke biomarker. Aims: To investigate the diagnostic value of soluble tumor necrosis factor-like weak inducer of apoptosis in patients with acute ischemic stroke and examine the relationship between ischemic area volume determined at diffusion-weighted magnetic resonance imaging and soluble tumor necrosis factor-like weak inducer of apoptosis. Study Design: A prospective, case-control study. Methods: This case-control prospective study included 36 patients with acute ischemic stroke and 36 healthy volunteers. Information on age, sex, presence of chronic disease, neurological examination findings, times of presentation to the emergency department after acute ischemic stroke, soluble tumor necrosis factor-like weak inducer of apoptosis levels, ischemic area volumes at diffusion-weighted magnetic resonance imaging, and 6-month mortality rates after stroke were recorded. The results were analyzed on SPSS 22.0 software (SPSS Inc., Chicago, IL, USA), and p<0.05 was considered statistically significant. Results: A soluble tumor necrosis factor-like weak inducer apoptosis cut-off value of 995.5 pg/mL exhibited a sensitivity of 80.5% and a positive predictive value of 82.5% with an area under the curve of 0.84 (95% confidence interval: 0.74-0.94; p<0.001). The mean soluble tumor necrosis factor-like weak inducer of apoptosis level - in the acute ischemic stroke group (1968.08=1441.99 mu g/L) were significantly higher than those in the control group (704.81 +/- 291.72 14,1) (p<0.001). No correlation was observed between soluble tumor necrosis factor-like weak inducer of apoptosis levels and ischemic area volume measured at diffusion-weighted magnetic resonance imaging (r=-0.008; p=0.07). The mean ischemic area volume was 505.68 +/- 381.10 and 60.96=80.89 mm(3) in the nonsurviving and surviving patients, respectively (p =.002). Conclusion: Soluble tumor necrosis factor-like weak inducer of apoptosis can be used in the diagnosis of acute ischemic stroke. However, it is inconclusive in estimating ischemic area volume and early mortality following acute ischemic stroke. Ischemic area volume measured at diffusion-weighted magnetic resonance imaging is a marker of poor prognosis and can be used in predicting early mortality.Öğe Does iron-deficiency anemia affect M1 macrophage activation and inflammation?(2020) Karahan, İrfan; Çifci, Aydın; Badem, Nermin DindarAim: Iron deficiency anemia (IDA) is a prevalent disorder and may be a problem for various systems. Anemia of inflammation has been extensively investigated before, but there is still a lack of knowledge about macrophage activation in IDA. Hence, the aim of this study was to investigate the relationship between IDA and macrophage activation. Patients and Methods: The present study included 88 female subjects. The participants were divided into two groups: 48 IDA patients in the patient group and 40 healthy subjects in the control group. M1 macrophage activation was measured with the triggering receptor expressed on myeloid cells-1 (TREM-1). TREM-1 levels and C-reactive protein were compared between patient and control groups. The relationship between TREM-1 levels and hemogram parameters and iron status was investigated. Results: TREM-1 levels of the patient group were significantly higher than of the control group [124.5 (6.8-770.5) pg/ml vs 48.5 (0.66-401.1) pg/ml, p=0.02], while CRP levels remained similar between the groups. There was no correlation between TREM-1 levels and hemoglobin, mean erythrocyte volume, ferritin, transferrin saturation and serum iron (p =0.96, 0.14, 0.21, 0.16, and 0.26, respectively) in IDA patients. Conclusion: The present study showed that IDA might increase TREM-1 levels and this condition might be a clue of macrophage activation. IDA patients should be considered in terms of pro-inflammatory conditions and further investigations are needed to clarify the association mentioned above.Öğe Effect of Various Laser Systems in Smokers and Non-smokers with Peri-implantitis: A Randomized, Prospective, Single-blind Clinical Trial(Galenos Publ House, 2023) Barış, Kubilay; Olgun, Ebru; Badem, Nermin DindarObjective: The aim of this study was to evaluate the effects of different laser systems in smokers and nonsmokers with peri-implantitis. Materials and Methods: Subjects were divided into six groups according to the study protocol: Group 1: Smokers who underwent diode laser application; group 2: Erbium, chromium: Yttrium, scandium, gallium, garnet (Er, Cr: YSGG) laser application. Group 3: smokers undergoing Erbium: Yttrium-aluminum-garnet (Er: YAG) laser application; group 4: non-smokers undergoing diode laser application; group 5: non-smokers undergoing Er,Cr: YSGG laser application; and group 6: non-smokers undergoing Er: YAG laser application. Peri-implant sulcus depth (SD), clinical attachment level (CAL), suppuration, modified plaque index (mPI), gingival index (GI), and modified sulcus bleeding index (mSBI) were recorded, and peri-implant sulcus fluid (PISF) was collected to evaluate osteocalcin. Results: There were statistically significant differences in the baseline and six-month SD, CAL, mPI, GI, mSBI measurements, and osteocalcin values in all groups (p<0.05). Conclusion: Laser applications for treating peri-implantitis have significantly improved clinical parameters and PISF osteocalcin levels.Öğe EŞ ZAMANLI PCT VE CRP İSTENEN TOPLUM KÖKENLİ PNÖMONİ VE KOAH ALEVLENMESİ OLAN HASTALARDA BU PARAMETRELERİN KORELASYONU VE MALİYET ETKİLİLİK AÇISINDAN DEĞERLENDİRİLMES(2019) Badem, Nermin Dindar; Peker, Seydi Ali; Kaygusuz, SedatAmaç: Bakteriyel enfeksiyonlar önemli morbidite vemortalite nedenidir. Serum prokalsitonin ve C-reaktifprotein sistemik inflamasyon ve bakteriyel enfeksiyonlarıntanı, tedavi ve takibinde önemli belirteçlerdir. Buçalışmada nihai tanıları pnömoni ve/veya KOAHalevlenmesi olan hastalarda son zamanlarda enfeksiyontakibinde sıklıkla kullanılmaya başlanan prokalsitonin ileklasik belirteç olarak kullanılan C-reaktif protein düzeyleriarasındaki korelasyon araştırıldı. Ayrıca alt solunum yoluenfeksiyonu tanısı ya da yönetimi için C-reaktif proteininmi, prokalsitoninin mi maliyet etkililik açısından yararlıolduğunu saptamayı amaçladık.Gereç ve Yöntemler: Toplam 1948 hastadan elde edilmiş4712 örnekten eş zamanlı çalışılan prokalsitonin ve Creaktif protein sonuçları değerlendirildi. Örneklerden499’u (%10.6) KOAH alevlenmesi (Grup I), 1480’i(%31.4) pnömoni (Grup II) ve 2733 (%58)’i KOAHalevlenmesi ve pnömoni (Grup III) tanısı almış hastalarınörneklerinden oluşuyordu.Bulgular: C-reaktif protein ve prokalsitonin düzeyleriarasında pozitif güçlü korelasyon saptandı (rho=0.512,p<0.001). Yine prokalsitonin ve C-reaktif proteinarasındaki grup içi değerlendirmede Grup I, Grup II veGrup III’de pozitif güçlü korelasyon tespit edildi (sırasıylarho=0.539, p<0.001; rho=0.509, p<0.001; rho=0.511,p<0.001).Sonuç: Prokalsitoninin C-reaktif protein ile korelasyongösterdiği, enfeksiyonun ileri dönem takibinde yüksekmaliyetli prokalsitonin yerine C-reaktif protein ölçümününkullanılmasının uygun olacağı kanaatine varılmıştırÖğe Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice(Walter De Gruyter Gmbh, 2023) Arikan, Funda Bulut; Ulas, Mustafa; Ustundag, Yasemin; Boyunaga, Hakan; Badem, Nermin DindarObjectives The present study sought to examine the relationship of betatrophin with certain key enzymes, namely lactate dehydrogenase-5 (LDH5), citrate synthase (CS), and acetyl-CoA carboxylase-1 (ACC1), in insulin-resistant mice.Methods Eight-week-old male C57BL6/J mice were used in this study (experimental group n=10 and control group n=10). S961 was administered using an osmotic pump to induce insulin resistance in the mice. The betatrophin, LDH5, CS, and ACC1 expression levels were determined from the livers of the mice using the real-time polymerase chain reaction (RT-PCR) method. Moreover, biochemical parameters such as the serum betatrophin, fasting glucose, insulin, triglyceride, total cholesterol, and high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol levels were analyzed.Results The betatrophin expression and serum betatrophin (p=0.000), fasting glucose, insulin, triglyceride (p <= 0.001), and total cholesterol (p=0.013) levels were increased in the experimental group. In addition, the CS gene expression level was statistically significantly decreased in the experimental group (p=0.01). Although strong correlation was found between the expression and serum betatrophin and triglyceride levels, no correlation was found between the betatrophin gene expression and the LDH5, ACC1, and CS gene expression levels.Conclusions The betatrophin level appears to play an important role in the regulation of triglyceride metabolism, while insulin resistance increases both the betatrophin gene expression and serum levels and decreases the CS expression level. The findings suggest that betatrophin may not regulate carbohydrate metabolism through CS and LDH5 or lipid metabolism directly through the ACC1 enzyme.Öğe Kırıkkale Ve Çevresinde Ergen Bireylerde D Vitamini Düzeyi Değerlendirmesi(2019) Badem, Nermin DindarAmaç: Kırıkkale ve çevresinde yaşayan ergenlerde D vitamini düzeyininin yaş, cinsiyet ve ölçüm zamanına göre oluşturulmuş gruplardaki değişimini değerlendirmektir. Gereç ve Yöntem: Muhtelif endikasyonlarla D vitamini düzeyi ölçülmüş, yaşları 11-18 arasında 2672 ergen çalışmaya alındı. Ergenler yaş, cinsiyet ve ölçüm zamanına göre gruplara ayrıldı. D vitamini düzeylerine göre bireyler literatürlere uygun şekilde; D vitamini eksikliği (?20 ng/mL), D vitamini yetersizliği (21-29 ng/mL), optimal D vitamini düzeyi (30-100 ng/mL) şeklinde sınıflandırıldı. Bulgular: Çalışma grubunun 1747’si kızlardan (%65.4), 925’i erkeklerden (%34.6) oluşuyordu. Tüm grubun D vitamini düzeyi 15.54±8.59 ng/mL olmakla birlikte kızlarda 13.48±7.80 ng/mL, erkeklerde 19.42±8.67 ng/mL idi. Kız ve erkeklerin D vitamini düzeyleri anlamlı şekilde farklıydı (p<0.001). Kızların % 84.9’unda D vitamini eksikliği, % 12.1’inde D vitamini yetersizliği varken sadece % 3’ünde normal düzeyde D vitamini vardı. Bu sıralama erkeklerde % 59.5, % 31.4 ve % 9.2 şeklindeydi. D vitamini düzeyi kızlarda 11 yaştan 18 yaşa doğru daha da azalmakta olup her yaş grubunda erkeklerden anlamlı şekilde daha düşüktü. Aylara göre değerlendirmede özellikle temmuz, ağustos ve eylül aylarındaki D vitamini düzeyleri diğer aylara göre anlamlı şekilde yüksekti (p<0.001). Sonuç: Çalışmamızda ergenlik dönemindeki bireylerde D vitamini açısından yüksek oranda eksiklik/yetersizlik olduğunu ve bu eksikliğin her mevsimde görüldüğünü tespit ettik. Kızlarda bu eksiklik erkeklere göre daha belirgindi. Özellikle kızlara vitamin D takviyesi konusunda hassasiyet gösterilmelidir.Öğe Mısır Şurubunun Metabolizmada Oluşturduğu Anarşi Ve Hastalıklarla İlişkisi(2018) Boyunağa, Hakan; Badem, Nermin Dindar; Mortaş, TülayYüksek fruktoz içeren mısır şurubunun tüketimi son elli yılda tüm dünyada artmıştır. Hazır gıdalara bol miktarda ilave edilen ve içeriğinde yüksek fruktoz bulunduran mısır şurubu metabolizmada anarşiye yol açmaktadır. Fruktoz, bağırsak ve karaciğer hücre yüzeyinde bulunan kanallara (GLUT: glukoz transporter) yüksek afinitesi nedeni ile hızla karaciğer ve pankreas hücrelerine dolmaktadır. Katabolizmasında yer alan enzimlerin kinetik özelliklerinin diğer şekerlerden farklı olması nedeni ile karaciğerde yağ asidi ve kolesterol sentezine yönlenmektedir. Ayrıca aşırı alındığında katabolizmasında yer alan fruktokinaz enzimi nedeni ile ürik asit oluşumunu arttırmakta ve gut artriti, gut nefritine yol açabilmektedir. Yüksek fruktoz içeren mısır şurubunun aşırı tüketimine bağlı olarak, organ içi yağlanma, obezite, insülin direncinde artış, ürik asit artışı, hipertansiyon gibi birçok patoloji tetiklenmektedir. Üretimindeki izolasyon aşamalarında civa ve karbonil bileşikleri bulaşımı da farklı patolojilere yol açabilmektedir. Oluşturabileceği patolojiler göz önüne alındığında, glukoz gibi diğer şekerlerden farklı bir metabolizmaya sahip olan fruktozun mısır şurubuyla aşırı alınmasına çok dikkat edilmesi gerekmektedir.Öğe Serum levels of ischemia-modified albumin and prolidase in migraine subjects(2020) Say, Bahar; Badem, Nermin Dindar; Ergun, UfukObjective: Migraine is one of the most common neurological diseases with headache attacks. Although its pathophysiology is still unclear, it is thought to be multifactorial. Oxidative stress is one of the topics discussed in the pathophysiology of migraine. The aim of this study was to investigate ischemia-modified albumin (IMA) and prolidase levels, which are oxidative stress markers in migraine. Material and Method: In this study, 35 migraine and 35 healthy controls were included. Serum IMA and prolidase levels were measured in serum obtained after centrifugation from venous blood samples by ELISA. Prolidase and IMA in migraine patients were compared with the control group. Results: The mean age was 40.06±11.14 years in migraine (34 female, 1 male) and 34.31±8.26 in controls (31 female, 4 male). There was no significant difference between the groups in terms of age and gender (p=0.08, p=0.29). Interictal IMA and prolidase levels of migraine subjects were significantly higher than the control group (p=0.02, p<0.001). Prolidase was significantly higher in the attack period than the control group (p<0.001), but there was no significant difference in IMA levels (p=0.34). Prolidase and IMA levels during the attack were higher than an interictal period in migraine but there was no significant difference (p>0.05). Conclusion: Prolidase and IMA may be an indicator of oxidative stress in migraine patients. Prolidase, one of the oxidative stress indicators, also correlates with the frequency of attacks in migraine. It is thought that these markers may lead to antioxidant agent studies in the prophylactic treatment of migraine.Öğe Significance of thiol/disulphide homeostasis and ischemia modified albumin levels in chronic obstructive pulmonary disease(2019) Eroğlu, Oğuz; Badem, Nermin Dindar; Baccıoğlu, Ayşe; Cömertpay, Ertan; Neselioglu, Salim; Erel, ÖzcanObjectives: The severity of inflammation occurring during chronic obstructive pulmonary disease (COPD) isclosely associated with oxidative stress. The aim of this study was to investigate the diagnostic value ofThiol/disulphide homeostasis (TDH) and ischemia modified albumin (IMA) levels in evaluating oxidativestress in COPD patients.Methods: This prospective study was performed with COPD patients presenting to the Kırıkkale UniversityHospital and with healthy volunteers. Subjects’ demographic data (age, sex, body mass index, and smokingstatus), native thiol (NT), total thiol (TT), disulphide (Ds), IMA levels and Ds/NT, Ds/TT and NT/TT ratioswere recorded. Statistical analysis was performed with SPSS 21.0 software.Results: One hundred ninety subjects were enrolled in the study, 141 COPD patients and 49 healthy volunteers.No difference was determined between the patient and control groups in terms of age, sex or body mass index.The antioxidant markers; NT and TT levels and NT/TT ratio were significantly lower in the patient groupcompared to the control group (p < 0.001, p < 0.001, and p < 0.003, respectively). The oxidant markers; IMAlevels and Ds/NT and Ds/TT ratios were significantly higher in the patient group (p = 0.006, p = 0.003, and p= 0.003, respectively). Significant negative correlation was determined between antioxidant and oxidantparameters. Sensitivity values were NT: 87.2%, TT: 83.3%, Ds/NT ratio: 68.1%, Ds/TT ratio: 68.1%, and IMA:77.8%.Conclusions: TDH was impaired in favor oxidants in COPD patients. TDH parameters and IMA can be usedto monitor oxidative stress emerging in COPD.Öğe The diagnostic value of neurogranin in patients with carbon monoxide poisoning: Can it show early neurological damage?(W B Saunders Co-Elsevier Inc, 2021) Yesilyurt, Omer; Comertpay, Ertan; Vural, Sevilay; Eroglu, Oguz; Badem, Nermin Dindar; Cankaya, Imran; Bilgili, Yasemin KaradenizBackground and aim: Carbon monoxide poisoning is a toxicological emergency that causes neurological complications. High serum neurogranin can be detected in acute or chronic conditions where brain tissue is damaged. This study aimed to investigate the diagnostic value of serum neurogranin level and its role in demonstrating neurological damage in patients admitted to the emergency department with carbon monoxide poisoning. Materials and methods: The study was conducted prospectively on patients with carbon monoxide poisoning (patient group) and healthy volunteers (control group). Demographic characteristics and scrum neurogranin level of all participants and symptoms at admission, neurological examination findings, laboratory results, and Diffusion-Weighted Magnetic Resonance Imaging results of the patient group were recorded. We used an independent sample t-test to compare neurogranin levels and bivariate correlation analysis to compare the relationship between serum neurogranin levels and data belonging to the patient group. Results: Sixty eight participants (patient group, n- 36; control group, n- 32) were included in the study. Serum neurogranin level was significantly higher in patients with carbon monoxide poisoning (0.31 +/- 0.16 ng/ml) compared to control group (0.22 +/- 0.10 ng/ml) (p = 0.015). The mean Glasgow Coma Scale of the patients with carbon monoxide poisoning was 14.59 +/- 0.23, and of Diffusion Weighted Magnetic Resonance Imaging results were completely normal in 94.4% (n = 34). There was no correlation between serum neurogranin level and Diffusion Weighted Magnetic Resonance Imaging results (r = -0.011; p = 0.953). Conclusion: Serum neurogranin level may be a new diagnostic biomarker in patients admitted to the emergency department with carbon monoxide poisoning. The high serum neurogranin levels detected in patients with normal diffusion-weighted imaging after carbon monoxide poisoning suggest that there is neurological damage in these patients, even if imaging methods cannot detect it. (C) 2021 Elsevier Inc. All rights reserved.Öğe The Effectiveness of Wet Cupping Therapy andEvaluation of Changes in Lactic Acid Levels inPatients with Myofascial Pain Syndrome(2020) Aras, Müyesser; Badem, Nermin Dindar; Kültür, Turgut; Boyunağa, Hakan; Keskin, Esra Dilek; Rajput, Kaleem Ullah; Dursun, GamzeObjective: Wet cupping therapy is used as a traditional and complementary therapy with a wide application all around the world for especially pain conditions. The aim of this study was to evaluate the effectiveness of wet cupping therapy (WCT) and to assess the changes in lactic acid (LA) levels in isolated leucocyte of the venous and cupping blood samples in patients with myofascial pain syndrome (MPS). Material and Methods: Patients aged between 18 and 65, and diagnosed with MPS were included the study. The Visual Analogue Scale (VAS) was recorded baseline, at 1st and 3rd months, Nothingham Health Profile (NHP) and Beck Depression Index (BDI) scores were recorded at baseline and 1st month follow-up in patients. The level of LA in isolated leucocyte of venous and cupping blood were also measured before and after 15 days WCT. Results:There was a significant decrease in VAS level from baseline to 1 and 3 months (p<0.001) and in BDI, NHP from baseline to 1 month (p <0.05). We found a significant decrease in the concentration of LA in isolated leucocyte of cupping blood 15 days after WCT (p<0.05). The median level of LA in isolated leucocyte of venous blood was lower than the levels before WCT but it wasn’t statistically significant (p>0.05). Conclusion: In conclusion, WCT is found to be effective in clinical improvements of VAS, BDI and NHP scores in our study. This study also suggests that WCT may be beneficial for pain through reducing LA levels in cupping blood in patients with MPS.Öğe Urinary excretion of heat-shock protein 70 in patients with familial mediterranean fever(Bayrakol Medical Publisher, 2022) Yildiz, Eren; Gokceoglu, Arife Uslu; Badem, Nermin Dindar; Alioglu, BulentAim: In this study, we aimed to determine the urinary excretion of HSP70 in patients with familial Mediterranean fever (FMF). Material and Methods: Children with FMF attack-free period and FMF attack and healthy controls were enrolled in the study. Characteristics of patients and laboratory results were recorded. Urine HSP70 levels, urine creatinine were studied, and urinary excretion of HSP70 was calculated. Results: Mean levels of urine HSP70 in patients with FMF attack-free period were significantly lower than that of controls (p = 0.01). The mean urinary excretion of HSP70 was higher in patients with an FMF attack than that of patients with FMF attack free period, but similar with controls. The mean HSP70 and urinary excretion of HSP70 were not different in children with homozygous MEFV mutation, in children with M694V mutation and others (p > 0.05). There was negative correlation between urinary excretion of HSP70 and the age of patients and controls (r =-0.284, p < 0.01). There was no statistically significant correlation between urinary excretion of HSP70 and leukocyte counts, CRP, ESR, fibrinogen levels (p > 0.05). Discussion: Urinary excretion of HSP70 increased in FMF patients during the attack period as it is a stress factor. Lower levels of urinary excretion of HSP70 in FMF patients with attack-free period may be related to the anti-inflammatory activity of colchicine. Urinary excretion of HSP70 was negatively correlated with the age of pediatric patients. MEFV mutation type as homozygous mutation and M694V mutation did not affect levels of urinary excretion of HSP70.Öğe Why do multiple sclerosis and migraine coexist?(ELSEVIER SCI LTD, 2020) Hamamci, Mehmet; Gocmen, Ayse Yesim; Say, Bahar; Alpua, Murat; Badem, Nermin Dindar; Ergun, Ufuk; Inan, Levent ErtugrulBackground: Migraine coexistence, which is high in multiple sclerosis (MS), is reported. To better understand the etiology of the coexistence of MS and migraine and the outcomes of this relationship, the vitamin D, vitamin D-binding protein (VITDBP), vitamin D receptor (VITDR), high-sensitivity C-reactive protein (hs-CRP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant status (TAS), total oxidant status (TOS), and Oxidative Stress Index (OSI) values were examined in patients with the coexistence of relapsing-remitting multiple sclerosis (RRMS) and migraine. Methods: This study was conducted between January 1, 2019, and July 25, 2019, at the neurology and biochemistry clinics of two different tertiary hospitals simultaneously. Overall, 50 RRMS patients with migraine, 50 RRMS patients without migraine, and 50 healthy volunteers were included in the study. The participants' vitamin D, VITDBP, VITDR, hs-CRP, SOD, CAT, GSH-Px, TAS, TOS, and OSI values were measured. Results: The vitamin D and VITDR values of the RRMS patients with migraine were lower than those of the RRMS patients without migraine (respectively, p = 0.014, p < 0.001). There was no significant difference between the RRMS patients with and without migraine in terms of their VITDBP values (p = 0.570). The SOD, CAT, GSH-Px, and TAS values of the RRMS patients with migraine were lower than those without migraine (all p < 0.001). The hs-CRP and TOS values of the RRMS patients with migraine were higher than those without migraine (all p < 0.001). Conclusion: To the best of our knowledge, this is the first study on this topic to date. Based on the results, our study may shed light on the etiopathogenesis of the coexistence of MS and migraine and new treatments. However, more studies are needed to better understand the etiology of this relationship and its negative effects.
