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    Histomorphology of the Brunner's Glands in the Angora Rabbit
    (Medwell Online, 2010) Ergun, Emel; Ergun, Levent; Ozen, Asuman; Kurum, Aytul; Bayraktaroglu, Alev Guerol
    The study was aimed to demonstrate the distribution, morphological and histochemical properties of Brunner's glands in the small intestine of the Angora rabbit. The duodenum of 10 healthy animals of both sexes constituted the material of the study. The glands were composed of acini densely packed within the submucosa. The Brunner's glands contained two types of cells, namely, serous and mucous cells. Histochemical examination revealed that the mucous glands and secretory ducts did not react with the Periodic Acid-Schiff (PAS) stain, while serous glands were weakly PAS-positive. Furthermore, muscous glands reacted positively with alcian blue pH 2.5. When applied the combined aldehyde fuchsin-alcian blue pH 2.5 staining procedure, mucous glands were determined to be aldehyde fuchsin (-) and alcian blue (+). These results showed that while a limited amount of neutral carbohydrates was secreted in serous glands, the secretion of the ducts and mucous cells of the duodenal glands in the Angora rabbit was composed of acidic carbohydates with this staining properties of the duodenal secretion. Electron microscopic examination revealed the cytoplasm of mucous gland cells to be filled with electron light secretion granules. Fewer electron dense granules were determined to be present among these electron light granules. The electron dense granules were found within the apical cytoplasm of serous glands.