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    Cytotoxic, apoptotic and necrotic effects of starch coated copper nanoparticles on Capan 1 pancreatic cancer cells
    (Elsevier, 2023) Ilbasmis-Tamer, Sibel; Turk, Mustafa; Evran, Sefika; Boyaci, Ismail Hakki; Ciftci, Hakan; Tamer, Ugur
    In the present study we utilized a green synthesis approach by reduction route with ascorbic acid in the presence of starch as the capping agent. Here we aimed to generate a stable starch coating copper nanoparticles (Cu-SNPs) and to investigate their apoptotic and necrotic effects on Capan 1 pancreatic cancer cell line. The water soluble monodisperse starch-protected zero-valent copper nanoparticles were characterized by transmission electron microscopy, XPS, FTIR analysis. TEM images showed that the presence of metallic copper nanoparticles with anisotrophic morphologies and the sizes of 90% copper nanoparticles were in 50 nm and 80 nm range. Cetyl-trimethylammonium bromide (CTAB) coated copper nanoparticles was also synthesized to compare the stability of starch coating copper nanoparticles. It was also found that copper nanoparticles were significantly protected by starch coating. Cu-SNPs exhibited stronger antimicrobial effects on Staphylocossus and Enterococcus. In vitro cell culture studies demonstrated that Cu-SNPs were not cytotoxic on L929-fibroblasts and Capan 1 pancreatic cancer cell in all concentrations used here (6.25-100 ppm) in different extends. The cytotoxic, necrotic, and apoptotic effects of Cu-SNPs on the Capan 1 pancreatic cancer cell line have been exhibited. Furthermore, the micronucleus test and hemolytic activity test were also performed.
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    Glucose determination based on a two component self-assembled monolayer functionalized surfaceenhanced Raman spectroscopy (SERS) probe
    (Royal Soc Chemistry, 2014) Torul, Hilal; Ciftci, Hakan; Dudak, Fahriye Ceyda; Adiguzel, Yekbun; Kulah, Haluk; Boyaci, Ismail Hakki; Tamer, Ugur
    In this report, we present a new detection method for blood glucose, using gold nanorod SERS, a surface enhanced Raman scattering probe embedded in two component self-assembled monolayers (SAMs). Gold nanorod particles and a gold coated slide surface were modified with the two component SAMs consisting of 3-mercaptophenylboronic acid (3-MBA) and 1-decanethiol (1-DT). The immobilization of 3-MBA/1-DT surface-functionalized gold nanoparticles onto 3-MBA/1-DT modified gold-coated slide surfaces was achieved by the cooperation of hydrophobic forces. Two component SAM functionalized substrates were used as SERS probes, by means of the boronic acid and the alkyl spacer functional groups that serve as the molecular recognition and penetration agents, respectively. The SERS platform surface was characterized by cyclic voltammetry, contact angle measurements, AFM (atomic force microscopy) and Raman spectroscopy. Optimum values of the parameters such as pH, time and (3-MBA/1-DT) molar ratio were also examined for the glucose determination. The analytical performance was evaluated and linear calibration graphs were obtained in the glucose concentration range of 2-16 mM, which is also in the range of the blood glucose levels, and the detection limit was found to be 0.5 mM. As a result, the SERS platform was also used for the determination of glucose in plasma samples.