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    Evaluating ESWL related alterations in renal pelvis and proximal ureter by analayting of tissue hydroxyproline levels
    (Ondokuz Mayis Universitesi, 2015) Fedakar Senyucel M.; Boybeyi O.; KemalAslan M.; Soyer T.; Balci M.; IzzetDemet A.; Cakmak M.
    The aim of the study is to evaluate extracorporeal shock wave lithotripsy (ESWL) related alterations in renal pelvis and proximal ureter by using histopathological methods and analayting hydroxy-proline levels. Twelve New-Zealand rabbits were allocated into two groups (n=6). Right sites of control group (CG, n=6) were harvested without any intervention. In ESWL group (EG), right kidneys of subjects were exposed to 3000 shock waves (14 kV) by using electro-hydraulic type ESWL device three times. Rabbits in EG were sacrificed on day 7. Tissues were examined histopathologically for presence of edema, inflammation, congestion, hemorrhage, fibrosis, vascularization and biochemically for hydroxyproline concentrations. Histopathologically, tissue edema was increased in renal pelvises and inflammation was increased in ureters in the EG compared to that in the CG (p<0.5). There was no difference in other parameters between the groups (p>0.05). Tissue collagen density did not show any significant difference (p>0.05). There was no difference in the tissue hydroxyl-proline levels of ureter samples (p>0.05). Tissue hydroxyproline levels were significantly higher in EG than CG in renal pelvis (p<0.05). In conclusion, although no major histopathological alteration due to ESWL was detected in renal pelvis and proximal ureter, increased hydroxyproline levels in the renal pelvis can be suggested as a finding of tissue injury in collecting system. © 2015 OMU.
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    In vitro evaluation of E. coli and S. epidermidis adhesion on dressing materials used in hypospadias repair
    (Springer Verlag, 2014) Soyer T.; Bostanoğlu E.; Aslan M.K.; Boybeyi O.; Demirbilek M.; Göçmen J.S.
    Background: An in vitro study was performed to evaluate the adherence of Escherichia coli and Staphylococcus epidermidis to different dressing materials. Methods: Hydrophyl gauze swab (GS), sterile flax swab (FS), and their nitrofurazone (Furacin®) absorbed forms (GSF and FSF), sterile paraffin-covered gauze with chlorhexidine acetate (0.5 %) (Bactigrass®, BG), and semipermeable transparent film gauze (Tegaderm®, TG) were used in the study. A 1 cm 2 of each material was placed in culture plates, and E. coli ATCC 25922 and S. epidermidis ATCC 12228 were suspended in each plate. After 24, 48 h, and 1 week of incubation period, colony counts were calculated in colony-forming units (CFU) per square centimeter unit. Bacterial adhesion was compared between groups, and each dressing material was also compared for different incubation periods (24, 48 h, and 1 week). Results: The colony counts were significantly decreased in GSF and FSF groups (Kruskal-Wallis test, p < 0.05). There was no difference for E. coli adhesion in GS, FS, BG, and TG groups (p > 0.05). The significant difference detected for S. epidermidis adhesion in the first 24 to 48 h could not be detected at the end of a 1-week period. There was no statistical difference in groups when compared for 24-, 48-h, and 1-week periods of incubations (Wilcoxon signed-rank test, p > 0.05). In BG group, E. coli adhesion was increased, and S. epidermidis adhesion was decreased without a significant difference at the end of a 1-week period (p > 0.05). Conclusions: Nitrofurazone absorbed sterile gauze materials have less bacterial adhesion. In vitro adhesion of E. coli and S. epidermidis did not differ between the 24-h and 1-week period. E. coli adhesion to BG can be increased if the duration of dressing prolonged up to a 1-week period.Level of Evidence: Not ratable. © 2013 Springer-Verlag Berlin Heidelberg.
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    Öğe
    Re: Evaluation of contralateral kidney, liver and lung after extracorporeal shock wave lithotripsy in rabbits
    (Elsevier Inc., 2013) Senyucel M.F.; Boybeyi O.; Ayva S.; Aslan M.K.; Soyer T.; Demet A.I.; Cakmak M.A.
    [No abstract available]
  • Küçük Resim
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    Serum procalcitonin level in monitorization of surgical site infections in neonates
    (Ondokuz Mayis Universitesi, 2016) Boybeyi O.; Soyer T.; Akbas O.T.; Guzoglu N.; Aslan M.K.; Gunal Y.D.; Aliefendioglu D.
    A retrospective study was performed to evaluate the role of white blood cell (WBC), C-reactive protein (CRP) and procalcitonin (PCT) levels in the follow-up and treatment of surgical site infections (SSI) in neonates. Neonates who underwent surgical intervention (n: 34) were evaluated for gestational age, sex, diagnosis, inflammatory markers (WBC, CRP, PCT), clinical findings, results of cultures and response to antimicrobial treatment. Records of 34 neonates and 36 surgical interventions were included to the study. Twenty (58.8%) of patients had SSI. Postoperatively CRP, WBC and PCT levels were increased (77%, 77%, 77% respectively) in patients with SSI. Postoperatively CRP, and WBC levels were increased (57% and 64% respectively) in patients without SSI. In conclusion, CRP levels and WBC count were significantly increased in 77% of cases after surgery. Increased PCT levels were detected only in patients with SSI. PCT levels may be considered as the most valuable marker to monitor SSI in neonates after surgery. © 2016 OMU.