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Öğe The expression of GST and CYP isoenzymes in thyroid nodular hyperplasia and papillary thyroid cancer tissue: Correlation with clinical parameters(Entomology & Applied Science Research Letters-Easletters, 2016) Oguztuzun, Serpil; Ergn, Duygu; Kilic, Murat; Bozer, Busra; Simsek, Gulcin Guler; Bulus, HakanThis study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) pi(P), mu(M), theta(T), omega(O) and kappa(K) cytochrome P450 (CYP) A1, B1 and 2E1 isoenzymes in thyroid nodular hyperplasia (NH) and papillary thyroid cancer (PTC) tissues. For immunohistochemical studies, tissues from 18 patients with thyroid nodular hyperplasia, 28 patients with papillary thyroid cancer at the Kecioren Training and Research Hospital, Ankara, Turkey, were used. Relationships between GST and CYP isoenzyme expressions in NH and PTC tissues were examined by the Mann-Whitney U test, and clinicopathological data were examined by the Pearson Correlation Test and Regression Analysis. When the NH and PTC tissues from these cases were compared with respect to their staining intensity, GSTP1, GSTO1, GSTK1, CYP1A1, CYP2E1 expressions in PTC cells were significantly higher than those in NH epithelial cells (p< 0.05). There were no statistically significant differences in the CYP1B1, GSTT1 and GSTM1 expressions between benign and tumor epithelium (p> 0.05). There were significant association between GSTO1, GSTK1 expressions and sT3 levels in PTC (p< 0.05) and CYP1B1 expression in NH. There was a significant association between GSTO1 expression and smoking status in NH ( p< 0.05). There was no statistical relationship between the GSTM1, GSTT1, GSTP1, GSTO1, GSTK1, CYP1A1, CYP1B1, CYP2E1 isoenzyme expressions and the clinicopathological data (age, TSH, sT4 levels, tumor stage) ( p> 0.05). GSTP1, GSTO1, GSTK1, CYP1A1 and CYP2E1 isoenzymes may have roles in the carcinogenesis of the papillary thyroid cancer.Öğe Expressions of CYP and GST Isoenzymes in Human Gastric Tumor and Non-Tumor Tissues(Akad Doktorlar Yayinevi, 2018) Simsek, Gulcin G.; Oguztuzun, Serpil; Bozer, Busra; Kilic, Murat; Kocdogan, Arzu K.; Kaygin, Pinar; Bulus, HakanIn this study we investigated the immunohistochemical staining characteristics of cytochrome P450 1A1 (CYP1A1), CYPB1, CYP2E1 and glutathione S-transferase P1 (GSTP1), GSTT1, GSTO1, GSTK1 isoenzymes in gastric tumor and surrounding tumor free (normal) gastric tissues from 40 patients. For immunohistochemical studies, tissues were obtained from 40 patients with gastric adenocarcinoma. Tumor and non-tumoral control tissues of patients were compared according to their staining intensity. Relationships between CYP and GST isoenzyme expressions in adenocarcinoma tissues were examined by the Mann Whitney-U test, and the clinicopathological data were examined by the Spearman's Rank Correlation test. CYP1B1, GSTT1, GSTO1 and GSTK1 expressions in gastric cancer cells were significantly higher than those in gastric normal epithelial cells (p < 0.05). However, CYP1A1, CYP2E1 and GSTP1 expressions were not significantly higher in tumor epithelium than those in normal epithelium in human gastric adenocancer (p < 0.05). Among the studied CYPs and GSTs, there was not statistically significant association between the studied isoenzyme expressions and age, gender and tumor grade ( p > 0.05). In patients with gastric adenocarcinoma, CYP1B1, GSTO1, GSTT1, and GSTK1 protein expressions are higher in tumor than normal gastric tissues.Öğe Expressions of glutathione S-transferase alpha, mu, pi, and theta in the skin samples of patients with acne rosacea(WILEY, 2020) Takci, Zennure; Bilgili, Serap Gunes; Kilic, Murat; Oguztuzun, Serpil; Bozer, Busra; Simsek, Gulcin Guler; Akbayrak, AtiyeBackground Data point to the importance of oxidative stress in rosacea. Glutathione S-transferases (GSTs) have substantial roles in a wide variety of oxidative stress-related conditions. Aim To evaluate the immunohistochemical staining characteristics of GST alpha (GSTA), mu (GSTM), pi (GSTP), and theta (GSTT) in patients with rosacea. Patients/Methods The study included 23 women and 7 men with rosacea (mean +/- SD age 49 +/- 11 year) and 15 healthy control subjects (10 women, 5 men; mean +/- SD age 47.86 +/- 10.88 year). For each patient, the average disease duration, disease subtype, ocular involvement, and severity score were recorded. A 3-mm punch biopsy was taken from the facial skin of each patient and control. Expression of GST isoenzymes was analyzed immunohistochemically. Results Expressions of GSTM1, GSTP1, and GSTT1 were significantly elevated in patients with rosacea compared to those in the control group (P = .0001,P = .0002,P < .0001, respectively). In the rosacea group, GSTT1 expression was significantly stronger than GSTP1 and GSTA1 expressions (P = .019,P < .0001, respectively). There were no significant associations between expressions of GST isoenzymes and gender, age, average duration of illness, disease subtype, ocular involvement, or severity score in the patient group (allP > .05). Conclusions In rosacea, the significant increase of GSTT1, GSTP1, and GSTM1 expressions might result from activation of GST as an outcome of extreme free radical generation from triggered neutrophils or ultraviolet vulnerability. These findings support the relevance of oxidant stress in the pathogenesis of rosacea.Öğe Glutathione S-transferase expression in benign and malignant eyelid tumors(Taylor & Francis Ltd, 2022) Saygin, Efe; Karadag, Remzi; Ozkanli, Sidika Seyma; Bozer, Busra; Oguztuzun, Serpil; Azari, Amir A.; Saygin, Isilay OzsoyEyelid tumors commonly originate from the skin and its appendages. Environmental toxins and oxidants affect eyelid carcinogenesis. Glutathione S-transferases (GST) are antioxidants that participate in pathogenesis. We investigated GST levels in malignant and benign eyelid tumors in otherwise healthy individuals. We used 57 malignant eyelid biopsies, benign eyelid biopsies, and tissue removed during blepharoplasty and entropion operations culled from pathology archives. Specimens were divided into three groups: malignant lesions, benign lesions and controls consisting of eyelid tissue removed during routine blepharoplasty and entropion surgery. Specimens were immunostained for seven GST (GST-A, GST-P, GST-Z, GST-S, GST-K, GST-O, GST-T) and the intensity of staining was quantified. In the malignant group, GST-O and GST-P staining was less intense than for the control group. In the benign group, the GST-P level was less than for the control group. We found no significant difference between the intensity of staining in malignant and benign groups. Our findings suggest that GST-O and GST-P enzymes may play significant roles in eyelid carcinogenesis.Öğe Investigation of Glutathione S-Transferase Isoenzyme Protein Expression in Patients With Pterygium(Lippincott Williams & Wilkins, 2016) Karadag, Remzi; Bayram, Nurettin; Oguztuzun, Serpil; Bozer, Busra; Bayramlar, Huseyin; Simsek, Gulcin Guler; Rapuano, Christopher J.Purpose: We investigated glutathione S-transferase (GST) enzymes in terms of their potential effects on the pathogenesis of pterygium. Methods: Twenty-six pterygium specimens and 15 normal conjunctival specimens of 15 control subjects were investigated. Expressions of GST (alpha, mu, pi, and theta) enzymes were assessed by immunohistochemical staining. A brown color in the cytoplasm and/or nuclei of epithelial cells was evaluated as positive staining for GST enzymes. For each antibody, the intensity of the reaction [negative (-), weak (1+), moderate (2+), or strong (3+)] was determined to describe the immunoreactions. Results: The median age was 52 years in the both groups. There was no significant difference between the groups in terms of age, sex, and intraocular pressure measurements (P > 0.05 for all). Of the 26 pterygium specimens, 15 (57.7%) (8 weak, 4 moderate, and 3 strong staining) were identified with GST pi-1 (GSTP1) expression and 20 (76.9%) (12 weak, 7 moderate, and 1 strong staining) with GST theta-1 (GSTT1) expression. Of the 15 control specimens, 4 (26.7%) (4 weak staining) were identified with the GSTP1 expression, and 1 (6.7%) with GSTT1 expression. GSTP1 and GSTT1 expressions were significantly higher in the pterygium specimens than in the controls (P = 0.043, P < 0.001; respectively). None of tissue specimens had positive staining for GST mu-1 or GST alpha-1 in both groups (both; P = 1.00). Conclusions: The significant increase of GSTP1 and GSTT1 expressions in pterygium may be because of the increased activation of GST in response to excessive free radical formation from ultraviolet exposure to maintain antioxidant capacity in pterygium.Öğe An investigation of human beta-defensins and cathelicidin expression in patients with pterygium(Consel Brasil Oftalmologia, 2017) Karadag, Remzi; Bayram, Nurettin; Oguztuzun, Serpil; Bayramlar, Huseyin; Bozer, Busra; Simsek, Gulcin; Rapuano, Christopher J.Purpose: To investigate human beta-defensins (HBDs) and cathelicidin LL-37 (LL-37) expressions in patients with pterygium. Methods: In this retrospective consecutive case series, 26 pterygium specimens and 15 normal conjunctival specimens of 15 control subjects were in-vestigated. Expressions of HBD-1, HBD-2, HBD-3, and LL-37 were assessed using immunohistochemical staining. A brown color in the cytoplasm and/or nuclei of epithelial cells indicated positive staining for HBDs and LL-37. For each antibody, the intensity of the reaction (negative [-], weak [1+], moderate [2+], or strong [3+]) was determined to describe the immunoreactions. Results: The median age was 52 years in both groups. There were no significant differences in age and sex between the groups (p=0.583, p=0.355, respectively). Of the 26 pterygium specimens, 15 (57.7%) (14 weak, 1 moderate staining) showed HBD-2 expression, which was not observed in any of the control specimens. One (3.8%) pterygium and one (6.7%) control specimen demonstrated weak staining for HBD-3. HBD-2 expression was significantly higher in the pterygium specimens than in the controls (p=0.002). None of the tissue specimens had positive staining for HBD-1 or LL-37 in either group (both; p=1.00). Conclusions: HBD-2 expression was higher in pterygium specimens than in the controls. HBD-2 expression that might be stimulated by inflammatory cytokines may be related to inflammation and fibrovascular proliferation and may play a role in pterygium pathogenesis.
