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    The effect of mesenchymal stem cell use on intra-abdominal adhesions in a rat model
    (Korean Surgical Society, 2018) Karaca, Gokhan; Pehlivanli, Faruk; Aydin, Oktay; Altunkaya, Canan; Uzun, Hafize; Niyaz, Mehmet; Bulut, Huri
    Purpose: Intra-abdominal adhesions (IAA) are among the most frequently seen pathologies in general surgery practice with an increased morbidity and mortality. In the present study, we investigated the effect of locally applied mesenchymal stem cells (MSCs) on IAA. Methods: Twenty-four Wistar Albino rats were used in the study. The rats were divided into three groups including: Sham, control, and MSCs group. On day 0, cecum was reached under anesthesia in all groups, except the Sham group. Scraping with a sponge was performed until petechial bleeding occurred. The control group received no treatment. In the stem cell group, MSCs were applied topically immediately after surgery on adhesions. The rats were sacrificed on day 10 and colon tissues and blood samples were collected for macroscopic, histopathological, and biochemical analysis. Results: In our study, E-selectin, P-selectin, TNF-alpha and IL-1 levels were statistically significantly lower in the MSC group than the control group, while the sham group has the lowest levels. In both the macroscopic and histopathological analyses (ZUhlke's scale), the least amount of adhesion was observed in the Sham group. In addition, although there was less adhesion in the MSC group than the control group, the difference did not reach statistical significance. Conclusion: Topical MSC application immediately after surgery suppresses the inflammatory process. However it was found to be ineffective in histopathological and macroscopic examinations performed on the 10th day.
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    FTIR, RAMAN and biochemical tools to detect reveal of oxidative Stress-Related lipid and protein changes in fibromyalgia
    (Elsevier, 2023) Guleken, Zozan; Suna, Gizem; Karaca, Sahika Burcu; Bulut, Huri; Ayada, Ceylan; Pancerz, Krzysztof; Paja, Wieslaw
    In this study, our aim was to investigate the pathogenesis and diagnosis of fibromyalgia (FM), a complex disorder with poorly understood causes. We focused on examining the role of oxidative stress and associated lipid and protein alterations in FM patients. To achieve this, we conducted a comprehensive analysis of serum samples obtained from 60 FM patients and 40 healthy individuals. In our analysis, we employed various biochemical assays and spectroscopic techniques including Fourier Transform Infra-Red (FTIR) and Raman spectroscopy. Moreover, we applied advanced statistical methods such as chemometrics and machine learning algorithms to analyze the collected data.The obtained results showed higher levels of oxidative stress, around 113% on the visual analogue scale score and around 5800% higher when C = O vibrations from lipids visible in FTIR spectra were analyzed. Also, lower levels of total antioxidants and oxidants in FM patients were observed compared with the healthy group.Moreover, FTIR spectra of serum collected from FM patients showed significantly higher absorbance of bands corresponding to polysaccharides, proteins, and lipids, while differences were not found in the Raman spectra. The principal component analysis (PCA) of the obtained spectroscopic data showed that it is possible to distinguish patients suffering from FM and healthy control groups with 100% accuracy using FTIR spectroscopy. PLS analysis showed significance in the differentiation of lipid vibrations among groups. In summary, FTIR coupled with chemometrics has the potential for fibromyalgia diagnosis.
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    Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
    (Mdpi, 2018) Pehlivanli, Faruk; Aydin, Oktay; Karaca, Gokhan; Aydin, Gulcin; Devrim, Tuba; Bulut, Huri; Daphan, Cagatay Erden
    Background: In this study, the effectiveness of dexpanthenol and coenzyme Q10 (CoQ10) on the healing of ischemic colon anastomosis was investigated. Methods: Forty eight maleWistar Albino rats were divided into four equal groups (Sham-S, Sham-I, DXP, Q10). Following full layer colon resection, single layer colon anastomosis, without creating ischemia, was performed on the Sham-S group. The same experimental model was performed on remaining groups after ischemia was created. Intraperitoneal dexpanthenol and CoQ10 was administered to the DXP and Q10 groups once a day for three days. Ten days later, all colon anastomoses were investigated histopathologically and biochemically, as well as their burst pressure values, in all sacrificed rats. Results: The highest burst pressure value was observed in the Sham-S group, decreasing from high to low in the DXP, Q10, and Sham-I groups, respectively (p = 0.008). Furthermore, tissue hydroxyproline (p = 0.001) level values were significantly different among the groups. Additionally, histopathological analysis revealed a significant difference among groups regarding reepithelization (p = 0.027) and polymorphonuclear leukocyte density (p = 0.022). Conclusions: This preliminary study has shown that ischemia-reperfusion injury may impair the healing of colon anastomosis and it has been concluded that dexpanthenol and CoQ10 may have positive effects on the healing of ischemic colon anastomosis in rat, although re-epithelization may be adversely affected using CoQ10.
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    May dexpanthenol, platelet-rich plasma, and thymoquinone provide new hope to maintain liver regeneration after partial hepatectomy?
    (2019) Aydın, Oktay; Pehlivanlı, Faruk; Karaca, Gökhan; Aydın, Gülçin; Altunkaya, Canan; Bulut, Huri; Aydınuraz, Kuzey
    Background/Aims: Complete liver regeneration may not always be possible after liver injuries and/or partial liver resection. The present study investigated the effects of dexpanthenol, platelet-rich plasma (PRP), and thymoquinone on liver regeneration in rats after partial hepatectomy (PH). Materials and Methods: A total of 34 Wistar albino rats, each weighing 250-280 g, were randomly separated into four groups. PH was performed, and except for the control group, intraperitoneal dexpanthenol, PRP, or thymoquinone was administered to the relevant groups for 7 days. All rats were then sacrificed, and the liver tissues were examined histopathologically and biochemically. Results: PRP reduced all oxidant-antioxidant parameters in rats that experienced liver regeneration, but did not create histopathological improvement in the liver tissue. Dexpanthenol had a histopathological improving effect on the liver tissue, but had no effect on biochemical parameters. Thymoquinone showed no histopathological or biochemical effects on liver regeneration. Conclusion: Although dexpanthenol did not affect biochemical oxidative parameters, it was considered to have improving effects on liver regeneration histopathologically. In addition, it was thought that PRP may be used for treatment of ischemia-reperfusion injury and cholestatic damage of the liver. Nevertheless, further studies are required on these subjects.
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    The Healing Effects of The Topical Mesenchymal Stem Cell Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2021) Ozden, Huseyin; Karaca, Gokhan; Bulut, Huri; Niyaz, Mehmet; Gomec, Muhammed; Daphan, Cagatay E.
    Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.
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    The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury
    (Veteriner Fakultesi Dergisi, 2021) Özden, Hüseyin; Karaca, Gökhan; Bulut, Huri; Niyaz, Mehmet; Gömeç, Muhammed; Daphan, Çağatay E.
    Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs. © 2021, Veteriner Fakultesi Dergisi. All rights reserved.