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Öğe The effect of ischemia reperfusion on intestinal contractility regulated by the nitrergic system(Scientific Technical Research Council Turkey, 2004) Caglayan, F; Sahin, A; Gunel, E; Cakmak, M; Caglayan, OBecause of the interaction between nitric oxide (NO) and the superoxide anion radical, the effect of ischemia-reperfusion on intestinal contractility regulated by the nitrergic system was investigated in the present study. The study was performed on 3 groups of rabbits: group 1, ischemia; group 2, ischemia and 1 h of reperfusion; group 3, ischemia and 24 h of reperfusion. Tissue samples were obtained from ischemic, ischemia-reperfused and adjacent uninjured intestines as study and control samples. The effects of atropine, tetrodotoxin, L-NAME and L-arginine on the intestinal response to electrical field stimulation (EFS) were investigated. Guanethidine was used to minimize adrenergic activity. Tetrodotoxin and atropine prevented contractions. L-NAME enhanced the responses to EFS in all tissue samples except for the study tissue of group 2, and L-arginine reversed this contraction elevation. Group 2 study tissue response was as high as 170% of that of the control tissue in standard Krebs-Henseleit solution, and no change was seen on this level with L-NAME and L-arginine addition. The effects of tetrodotoxin and atropine revealed that EFS affects via the cholinergic neuronal system. Ischemia reperfusion affects intestinal contractility, especially in the early phases of reperfusion. In the light of the increased response to EFS and insensitivity to L-NAME and L-arginine of the affected tissue during this period it was thought that the nitrergic system is considerably affected by ischemia reperfusion. Excessive production of superoxide anion radicals or reversible inhibition of nitric oxide synthase may be the cause of this.Öğe The effects of anesthetic techniques on acute phase response at delivery (anesthesia and acute phase response)(Pergamon-Elsevier Science Ltd, 2003) Buyukkocak, U; Caglayan, O; Oral, H; Basar, H; Daphan, CObjectives: To investigate the effects of anesthetic techniques and delivery types on stress response during uncomplicated delivery. Design and methods: Forty pregnant women at term were divided into four groups. Group-I, cesarean section with general anesthesia; Group-II, section with spinal anesthesia; Group-III, section with epidural anesthesia; and Group-IV, vaginal delivery with epidural analgesia. C-reactive protein and albumin were measured on hospital admission, immediately after delivery and 24 h later. Results: Albumin and CRP levels decreased significantly in Group-II and Group-III, just after delivery. CRP increased significantly in all groups at the 24(th) hour of delivery. The effect of hemodilution on CRP and Albumin, due to volum loading, during spinal and epidural anesthesia disappeared after 24 h. Conclusion: We found no influence of anesthetic techniques on acute phase response except hemodilution effect. In the view of delivery types, vaginal delivery caused less stress response than section. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.Öğe Protective effects of trapidil in lung after abdominal aorta induced ischemia-reperfusion injury: an experimental study(Springer, 2005) Somuncu, S; Cakmak, M; Erdogan, S; Caglayan, O; Caglayan, F; Akman, H; Kaya, MWe aimed to investigate the protective effects of trapidil after the occlusion of abdominal aorta and the reperfusion injury in lung. Eighteen New Zealand albino rabbits were used in the study. In six animals [group 1, ischemia-reperfusion (IR) group], the abdominal aorta was exposed and a microvascular clamp was placed in the infrarenal abdominal aorta for 60 min. After the ischemic period, the microvascular clamp was removed and reperfusion was provided for 2 h. After the reperfusion period, the lungs were removed carefully and specimens were prepared for histopathological and biochemical studies in appropriate conditions. In group 2 (study group), trapidil (Rocarnal, Rentschler-UCB GmbH, Kerpen, Germany) was administered intraperitoneally as a single dose 1 h prior to trial, the IR procedure was performed and lung specimens were prepared similar to group 1. In group 3 (sham group), the infrarenal abdominal aorta was exposed and lung specimens were prepared for histopathological and biochemical studies at the end of the study. Histopathological changes, malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl group (T-SH) levels were evaluated. There was a statistical difference between the IR group and study group regarding NO and MDA levels (P < 0.05 and P < 0.01, respectively), but this was not detected between the IR group and the sham group (P > 0.05). There was no statistical difference among the three groups regarding T-SH levels (P > 0.05). While a statistical difference was found between the sham group and study group in the NO level (P < 0.05), no statistical difference was found in the MDA level (P > 0.05). There was a statistical difference in interstitial edema, PMN infiltration and hemorrhage scores among the groups (P < 0.05). There was a statistical difference between the IR group and study group in PMN infiltration (P < 0.05), but this was not detected between the groups in interstitial edema and hemorrhage scores (P > 0.05). There was a statistical difference between IR group and sham group in interstitial edema, PMN infiltration and hemorrhage scores (P < 0.05). Statistical difference was found between the sham group and study group in interstitial edema and hemorrhage scores (P < 0.05), but not in PMN infiltration (P > 0.05). Conclusions: Infrarenal abdominal aortic occlusion and reperfusion causes lung injury. We conclude that trapidil has preventive effects in the lung tissue after IR injury.Öğe Relationship of serum adiponectin level, anthropometric parameters and bone mineral density in postmenopausal(Springer London Ltd, 2005) Ozkurt, B; Ozkurt, ZN; Altay, M; Aktekin, CN; Caglayan, O; Tabak, Y…Öğe Testicular tissue nitric oxide and thiobarbituric acid reactive substance levels: evaluation with respect to the pathogenesis of varicocele(Springer, 2004) Kisa, U; Basar, MM; Ferhat, M; Yilmaz, E; Basar, H; Caglayan, O; Batislam, EThe aim of the present study is to evaluate tissue nitric oxide (NO) and thiobarbituric acid reactive substance (TBARS) levels in testicular tissue, and to determine their relationship with seminal parameters in order to explain possible effects on varicocele pathophysiology.Ten adult male Wistar rats at 8 weeks old underwent partial left renal vein ligation. A sham operation was performed on control rats in a second group of another ten rats. All animals were killed 4 weeks after surgery. The testes were removed and histological changes were observed by light microscopy with haematoxylin and eosin stain on half of each testis. The rest of testis was used for the evaluation of testicular tissue NO and TBARS levels. Epididymal aspirated seminal plasma was used for semen analysis and morphological analysis was carried out according to Kruger's criteria. Statistical analysis was performed by using Mann-Whitney U-tests and Spearman rank correlations between the two groups for NO and TBARS levels and for seminal parameters. Testicular tissue NO and TBARS levels (mean+/-SEM) were 62.8+/-10.1 mumol/g protein and 4.7+/-0.3 nmol/g protein in group 1. These parameters were 16.9+/-2.2 mumol/g protein and 3.1+/-0.2 nmol/g protein in the group 2 controls. There were significant differences between these parameters (P-NO=0.000, P-TBARS=0.001). Although a positive and significant correlation between testicular tissue NO and TBARS levels was found (r(s)=0.739, P=0.014), there was only a strong negative correlation between NO levels and sperm motility in group 1 (r(s)=-0.815, P=0.004). We found that this effect of NO on sperm motility was independent from TBARS levels after regression analysis (r(2)=-0.687, beta=0.825, P=0.034). Although there were statistically significant differences in seminal parameters between the two groups, there was no difference between them in the histopathological examination. We found that sperm motility was significantly related to testicular tissue NO levels only. Thus, we suggest that NO is an important mediator in the pathogenesis of varicocele. TBARS and other substances have been effective via NO pathways.
