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Öğe Acute and chronic impact of smoking on salivary and serum total antioxidant capacity(Pakistan Medical Assoc, 2015) Kurku, Huseyin; Kacmaz, Murat; Kisa, Ucler; Dogan, Ozlem; Caglayan, OsmanObjective: To investigate increased oxidative stress in saliva of smokers along with their serum. Methods: The case-control study was conducted from September to December 2008 in the Department of Biochemistry of the Medical School, Kirikkale University, Kirikkale, Turkey. A blood sample and saliva samples before and after smoking were collected from the smokers, while blood and saliva samples were taken from the controls. All samples were taken concurrently. The samples were measured for total antioxidant capacity, total oxidant stress, oxidative stress index, malondialdehyde, nitric oxide and total sulfhydryl groups. Levels of superoxide dismutase and glutathione peroxidase were also measured on saliva samples. SPSS 13 was used for statistical analysis. Results: Of the 54 subjects in the study, 27(50%) were smokers with a mean age of 28.4+/-5.42 years, and 27(50%) were controls with a mean age of 29.7+/-8.03 years. Total oxidant stress, oxidative stress index, malondialdehyde and nitric oxide levels were found higher in the serum samples of smokers (p<0.05), and the levels of total sulfhydryl groups in smokers were lower compared with the controls (p<0,05). Among the smokers, salivary malondialdehyde levels were higher before and after smoking (p<0.05), glutathione peroxidase levels were lower than the controls, and salivary nitric oxide levels after smoking were higher than both those of the control group and the levels before smoking (p<0.05). Conclusion: Both acute and chronic increased oxidative conditions may be a significant sign of the destructive effects of smoking. The investigation of disorders in smokers concerning oxidative stress will be beneficial in terms of novel approaches and treatment modalities.Öğe Can Resistin be a New Indicator of Neonatal Sepsis?(Elsevier Taiwan, 2014) Aliefendioglu, Didem; Gursoy, Tugba; Caglayan, Osman; Aktas, Alev; Ovali, FahriBackground: Sepsis is an important cause of neonatal death and perinatal brain damage, particularly in preterm infants. It is thought that activation of the inflammatory cascade triggered by cytokine might play a role in the pathogenesis of sepsis. Recent evidence supports a role for resistin in inflammation. There are no data in the literature on resistin levels of premature newborns with sepsis, which can also cause inflammatory response. The objective of this study was to evaluate whether resistin can be used as an indicator in neonatal sepsis of preterm babies. Materials and methods: Forty-three premature newborns considered to have sepsis were included in the study. Forty-three gestational and postnatal age- and sex-matched premature newborns without premature prolonged rupture of membrane or sepsis served as controls. Results: The median resistin and interleukin-6 (IL-6) levels of the premature babies with sepsis were 85.9 ng/mL and 342.7 pg/mL, respectively, and were higher than those of the control group (29.9 ng/mL and 17.7 pg/mL, respectively). The sensitivity, specificity, positive, and negative predictive values for resistin were 73.7%, 45.8%, 68.3%, and 52.4%, respectively. Conclusion: Resistin levels were higher in premature newborns with sepsis and correlated with IL-6 levels, which is an indicator of neonatal sepsis. This suggests that resistin may also be used in the diagnosis of neonatal sepsis. However, it has limited value when compared with the other inflammatory markers including C-reactive protein, procalcitonin, and IL-6. Copyright (C) 2013, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. All rights reserved.Öğe Celiac disease prevalence in patients with iron deficiency anemia of obscure origin(Turkish Soc Gastroenterology, 2009) Ucardag, Derya; Guliter, Sefa; Ceneli, Ozcan; Yakaryilmaz, Fahri; Atasoy, Pinar; Caglayan, OsmanBackground/aims: Anemia, especially due to iron deficiency, is a frequent feature in celiac disease. In this study, we aimed to define the prevalence of celiac disease in Turkish patients with iron deficiency anemia of obscure origin. Methods: One thousand four hundred and eighty-six consecutive patients with iron deficiency anemia were evaluated for etiology. Of those, 77 patients were found to have iron deficiency anemia of obscure origin. Sera from 77 patients with iron deficiency anemia of obscure origin and 119 healthy controls were tested for IgA and IgG tissue transglutaminase (tTG) antibodies by ELISA. Endoscopic mucosal biopsies were taken from the second part of the duodenum in these patients. Histopathologic examination results of patients were stratified according to Marsh classification. Results: IgA and IgG class anti-tTG antibodies were found positive in 6 (7.8%) and 3 (3.9%) patients with iron deficiency anemia of obscure origin, respectively. Three patients had only IgA anti-tTG and 3 had both IgA and IgG anti-tTG antibodies. In the control group, 1 subject was positive for both IgA and IgG anti-tTG antibodies (0.7%). Six patients (7.8%) and 1 control subject (0.8%) had histopathologic findings of celiac disease (p=0.02). Conclusions: Patients with iron deficiency anemia of obscure origin had increased prevalance of celiac disease. Our study results suggest that serological screening may be recommended for early detection of celiac disease in patients with iron deficiency anemia of obscure origin.Öğe Comparison of hematological and biochemical parameters in sheep naturally and persistently infected with a border disease virus(Springer, 2011) Gazyagci, Serkal; Azkur, Ahmet Kursat; Caglayan, OsmanIn this study, we investigated the changes occurring in the activities of determining the biochemical and hematological parameters in persistently infected sheep with border disease virus (BDV) and control sheep. While cholesterol, aspartate aminotransferase, lactate dehydrogenase, high-density lipoprotein, and glucose parameters were found to be statistically different between control and BDV positive groups (p < 0.01), total protein, alkaline phosphotase, creatine kinase, amylase, glucose, and high-density lipoprotein were found to be statistically different between control and persistently infected group (p < 0.01). Interestingly, all groups were shown only mean corpuscular volume parameter was different (p < 0.01). It was found that cholesterol, aspartate aminotransferase, amylase, high-density lipoprotein, and low-density lipoprotein parameters were different between PI and infected sheep (p < 0.01). It was speculated that BDV might effect also the expression of low-density lipoprotein receptor and determination of the changes in BD and its clinical importance might contribute to the veterinarians and scientists studying in this area.Öğe The Effect of Hypothermia on Splanchnic Flows and Lung in a Two-Hit Hemorrhagic Shock Model(Academic Press Inc Elsevier Science, 2010) Deniz, Turgut; Agalar, Canan; Agalar, Fatih; Comu, Faruk M.; Caglayan, Osman; Alpay, Yesim; Saygun, OralBackground. To evaluate the effect of hypothermia on bacterial translocation, splanchnic vascular flow, lung tissue weight, and levels of malondialdehyde (MDA) and nitric oxide (NO) in a two-hit model of hemorrhagic shock. Methods. Thirty rats were randomly allocated into three groups of 10 rats each. In the control group (group C), rats were treated without hemorrhage, and normothermia (37 degrees C) was maintained. In the mild hypothermia group (group MH), rats were subjected to volume-controlled hemorrhage (2 mL/100g) and a rectal temperature of 34 degrees C was maintained. In the normothermic group (group NT), rats were treated as in group MH, except for hypothermia. Seventy-two hours after hemorrhagic shock (first insult), Pseudomonas aeuruginosa was administered intratracheally as a second insult. Finally, mesenteric vascular flow patterns were recorded. Bacterial translocation was studied from tissue samples of spleen, liver, and mesenteric lymph nodes. Blood samples were obtained to evaluate the possible presence of bacteria in the bloodstream. Lung tissue weight ratio, MDA, and NO levels in lung tissue were assessed. Results. Renal, mesenteric, and portal venous flow rates were found to be lower in groups Mill and NT in comparison with group C. Blood flow profiles were lower in group NT than in group MH (P < 0.05). Bacterial translocation was not observed in group C, and it was detected more often in group NT than in group MH. Lung weight ratio was found to be higher in group NT compared with groups MH and C. Although it did not reach the level of statistical significance, MDA level in the control group was lower than that in the NT group (P = 0.085). Conclusion. Hypothermia corrected mesenteric blood flow and decreased the occurrence of bacterial translocation in the two-hit model of hemorrhagic shock and tracheal inoculaton of P. aeruginosa (C) 2010 Elsevier Inc. All rights reserved.Öğe The effect of insulin resistance on mortality in critically ill patients in the intensive care unit(INDIAN ANAESTHETISTS FORUM, 2020) Bakkal, Ayse Gulcan; Buyuksekerci, Murat; Gencay, Isin; Aydin, Gulcin; Caglayan, Osman; Buyukkocak, UnaseBackground and Aims: Insulin resistance can be described as a subnormal biological response to a specific insulin concentration or deterioration of an accepted response to insulin in glucose homeostasis and deficiency of insulin response. The aim of this study is to evaluate the effect of insulin resistance on mortality in critically ill patients. Methods: Over 18-year-old and nondiabetic 150 patients that had been hospitalized in an intensive care unit (ICU) between September 2013 and October 2014 were enrolled in this study. The Acute Physiology and Chronic Health Enquiry II (APACHE II), Glasgow Coma Scale, and Richmond Agitation and Sedation Scale were calculated on the day of admission to the ICU, and following 4th day and 1st, 2nd, 3rd, and 4th weeks. Insulin resistance was calculated using the HOMA formula. Infection and other complications during ICU stay, the requirement of mechanical ventilation (MV), nutritional status (parenteral and/or enteral), vasopressor, steroid, and insulin treatment were also recorded. Patients followed in the ICU were recorded as survivors and nonsurvivors. Results: Glucose levels were found to be higher in nonsurvivor group at the 1st week and there was a significant positive relationship between APACHE II score and insulin resistance at the 3rd week. There was a significant relationship between mortality and requirement of MV, vasopressor medication, complications, and infection. Conclusion: We conclude that the effect of insulin resistance seems to affect the mortality in critically ill patients after at least a 3 weeks long follow-up time.Öğe Effect of phototherapy on gastrointestinal smooth muscle activity and oxidative stress(Springer, 2011) Soyer, Tutku; Aliefendioglu, Didem; Aktuna, Zuhal; Caglayan, Osman; Aydos, Tolga Resat; Cakmak, MuratAim To evaluate the effect of phototherapy on gastrointestinal smooth muscle activity and oxidative stress. Methods Wistar albino rats (n = 18, in the first 7 days of life) weighing 7 +/- 2 g with both sexes were included in the study. The animals were randomized into three groups. In control group (CG), median laparotomy was performed to obtain 1 cm of jejunum, terminal ileum and colonic segments. In the phototherapy group (PTG), led phototherapy with a wave density of 40 mu w/cm(2)/nm were used (Bilitron 3006, Fanem, Brasil). The efficacy surface of phototherapy was 30-40 cm and the exposure distance was 30 cm. The duration of phototherapy was 24 h. Sham group (SG) received white light with the same wave density and exposure distance. The oxidative stress markers and contraction responses were investigated from intestinal segments obtained from experiments. Results The jejunum segments showed significantly lowered contraction response to carbachol in SG when compared to CG and PTG (p < 0.05). Decreased contractile response to KCl was detected in both SG and PTG in terminal ileum segments. MDA levels showed no difference between groups (p > 0.05). Total sulfhydryl (T-SH) levels were found significantly increased in PTG when compared to CG and SG (p < 0.05). When NO levels were evaluated, NO levels were found decreased in PTG and SG with respect to CG (p < 0.05). Conclusion PT may cause various alterations in oxidant/antioxidant system in intestinal segments. Unlike to clinical findings, decreased contractile responses were detected in rat gastrointestinal smooth muscles after PT.Öğe The effects of early and late feeding on healing of esophageal anastomoses: An experimental study(Elsevier Ireland Ltd, 2011) Yurtcu, Muslim; Toy, Hatice; Arbag, Hamdi; Caglayan, OsmanObjective: To investigate the effects of postoperative late and early feeding on the healing of experimental esophageal anastomosis (EA). Methods: Twelve New Zealand rabbits were divided equally into 2 groups: late feeding (LF) and early feeding (EF). A 1-cm-length of the cervical esophagus was resected through a cervical incision, and then anastomosis was performed. The LF group was fed parenterally for 6 days and orally (per oral route) after esophagography on postoperative day 7 as long as there was no esophageal leakage. The EF group was fed orally (per oral route) 24 h after esophageal repairs (ERs), and esophagography was carried out on postoperative day 7 to check the ERs. The rabbits were sacrificed to evaluate bursting pressure (BP), diameter of the esophageal lumen (DOTEL), tissue hydroxyproline (HP), and wound healing scores (WHSs) in the anastomosis lines 8 weeks later. Results: In the LF group, BP and DOTEL were significantly lower than they were in the EF group, and HP was significantly higher than it was in the EF group. WHSs in the LF group were not higher than they were in the EFgroup. Conclusions: Early feeding is superior after EA as shown by increased DOTEL and BP levels, but causes decreased HP levels. (C) 2011 Elsevier Ireland Ltd. All rights reserved.Öğe Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats(Taylor & Francis Ltd, 2011) Arslan, Ayse; Orkun, Sevim; Aydin, Gulumser; Keles, Isik; Tosun, Aliye; Arslan, Mustafa; Caglayan, OsmanObjectives: The aim of this study is to investigate the effects of ovariectomy on bone mineral density (BMD) and oxidative state in rats, and the alterations in these effects that vitamin C supplementation may produce. Materials and methods: Twenty female Wistar albino rats were randomly divided into three groups: control (C, n = 6); ovariectomy (O, n = 7); and ovariectomy + vitamin C supplement (OV, n = 7). Oxidative stress (OS) was assessed 100 days postovariectomy by measuring the activity of several enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase, as well as the concentrations of malondialdehyde (MDA), nitric oxide (NO), and total sulfhydryl groups in plasma and bone homogenates. Results: A significant decrease in BMD was observed in O group compared with C group (p = 0.015), and a significant increase was observed in OV compared with O group (p = 0.003). When groups were compared with respect to parameters of OS, MDA and NO levels in bone tissue were significantly higher in O than in C (p = 0.032, p = 0.022) and were significantly lower in OV than in O (p = 0.025, p = 0.018). SOD activity was significantly higher in O than in C (p = 0.032). In plasma, MDA activity was significantly higher in O than in C (p = 0.022) and NO level was significantly higher in O than in C and OV (p = 0.017, p = 0.018). Conclusions: Our results suggest that ovariectomy may produce osteoporosis and OS in females, and vitamin C supplementation may provide alterations regarding improvement in OS and BMD values. We assume that studies including more subjects are needed to make a decisive conclusion about OS-BMD relation.Öğe Effects of platelet rich plasma on colonic anastomosis(Academic Press Inc Elsevier Science, 2008) Yol, Serdar; Tekin, Ahmet; Yilmaz, Hüseyin; Küçükkartallar, Tevfik; Esen, Hasan; Caglayan, Osman; Tatkan, YükselPurpose. To investigate the effect of platelet-rich plasma (PRP) on tissue maturation and wound healing in experimental colonic anastomosis. Materials and methods. Thirty Sprague Dawley rats were divided into three groups of 10 rats each. Group I (control group) was subjected to colon anastomosis only. Group II (PRP group) was subjected to colon anastomosis and topical PRP was applied. Group III (Bioglue group) was subjected to colon anastomosis and topical tissue sealant was applied (Bioglue; Cryo-life, Kennesaw, GA). The rats were sacrificed on postoperative day 7, and the bursting pressure of the anastomosis and tissue hydroxyproline levels were measured; histopathological changes on the anastomosis line were also examined. Results. The bursting pressure was statistically higher in the PRP group than in the control and Bioglue groups (P < 0.05). The hydroxyproline levels were also statistically higher in the PRP group than in the control and Bioglue groups (P < 0.05). Histopathologically, there was less inflammatory cell infiltration, intensive fibroblast development, and rich collagen production in the PRP group. Conclusion. PRP may be used in colon anastomosis, especially in patients with impaired wound healing, to obtain a better anastomotic strength. (c) 2008 Elsevier Inc. All rights reserved.Öğe Evaluation of adrenomedullin and endothelin-1: Are they factors in the adaptation of maternal vascular system in normotensive pregnancy(Springer, 2008) Bayram, Merih; Bostanci, Suhha; Caglayan, Osman; Ilhan, Mustafa N.Introduction: The aim of our study was to analyse the role of adrenomedullin (AM) and endothelin-1 (ET-1) in the adaptation of the maternal vascular system in normotensive pregnancy. Methods: Twenty-eight pregnant women, who were normotensive throughout the duration of their pregnancy, were recruited into the study. Plasma levels of AM and ET-1 at each trimester were measured and the AM/ET-1 ratio was calculated. Results: Our experiment showed a significant decrease in plasma concentrations of AM in the first trimester for the study group (n=28) compared with the non-pregnant control group (n=16). There was also a significant decrease in plasma concentrations of ET-1 in all three trimesters (P < 0.05) and a significant increase in the AM/ET-1 ratio in all three trimesters (P < 0.05) for the study group compared with the control group. Conclusion: An alteration in vascular equilibrium between AM and ET-1, favouring AM, may be a reason why the physiological adaptation of the maternal vascular system to pregnancy occurs during normotensive pregnancy.Öğe Evaluation of serum resistin levels in patients with ocular and non-ocular Behcet's disease(Canadian Ophthal Soc, 2008) Yalcindag, F. Niluefer; Yalcindag, Ali; Batioglu, Figen; Caglayan, Osman; Kisa, Ucler; Ozdemir, OzdenBackground: Resistin, a recently identified adipocytokine, has been found to play an important role in inflammation and the processes of inflammation-related diseases. Serum resistin levels in patients with Behcet's disease (BD) have not yet been investigated. We aimed to evaluate the relation between resistin and interleukin-6 (IL-6) in Behcet patients with or without ocular involvement and in normal controls. Methods: Twenty-two patients with BID and 19 healthy control subjects were included in this study. While 14 patients had posterior segment involvement of the eye, the other 8 did not have ocular disease. Serum resistin and interleukin-6 (IL-6), levels were measured in all samples. Data from all groups were tested for statistical significance. Results: The mean resistin and IL-6 concentrations were significantly higher in patients with BD than the control subjects (p = 0.011 and p = 0.0001, respectively). There was a significant difference in resistin and IL-6 levels between the patients with non-ocular BID and controls (p = 0.013 and p = 0.0001, respectively), as well as resistin and IL-6 levels between the ocular BD group and the control group (p = 0.05 and p = 0.0001, respectively). However, there was no significant difference between patients with ocular versus non-ocular BID.Öğe The healing effects of tissue glues and healing agent locally applied on esophageal anastomoses(Elsevier Ireland Ltd, 2010) Yurtcu, Mueslim; Arbag, Hamdi; Toy, Hatice; Eryimaz, Mehmet Akif; Caglayan, Osman; Abasiyanik, AdnanObjective: This study aimed to investigate the effects of cyanoacrylate (C), fibrin glue (FG), and natrium hyaluronate (NH) on the healing of esophageal anastomosis (EA). Methods: Twenty-four rabbits were divided equally into 4 groups: primary anastomosis (PA), C, FG, and NH. A 1-cm-length of the cervical esophagus was resected through a cervical incision and then anastomosis was performed. C, FG, and NH were instilled into anastomosis lines in the respective groups. The animals were fed orally on postoperative day 7 on the condition that there was no esophageal leakage. The rabbits were sacrificed 8 weeks later to evaluate bursting pressure (BP), tissue hydroxyproline (HP) levels and wound healing scores (WHSs) in the anastomosis lines. Results: BP was significantly higher in the C group than in the PA, FG, and NH groups, and HP was significantly lower than in the other groups. WHSs in the PA and NH groups were lower than in the C and FG groups. Conclusions: C and NH appear to be beneficial in EA healing with respect to increased BP and decreased HP when they are used simultaneously with PA prophylactically to prevent esophageal leakages and stricture. (C) 2009 Elsevier Ireland Ltd. All rights reserved.Öğe Impact of adiponectin on left ventricular mass index in non-complicated obese subjects(Japan Endocrine Soc, 2008) Ebinc, Haksun; Ebninc, Fatma Ayerden; Oezkurt, Zuebeyde Nur; Dogru, Mehmet Tolga; Tulmac, Murat; Yilmaz, Murat; Caglayan, OsmanTo evaluate the relationship between the adiponectin levels and left ventricular mass index (LVMI) in uncomplicated obese subjects. Fifty-nine subjects were assigned to the obese (BMI >= 30 kg/ml) and 58 to the lean (BMI<30 kg/m(2)) group. Plasma glucose, insulin, serum total cholesterol and high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, triglycerides and adiponectin were measured. Insulin resistance was determined by the Homeostasis Assessment Model (HOMA-IR). The left ventricular functions of all subjects were determined by 2D and pulse wave Doppler echocardiography. LVMI was calculated as left ventricular mass (LVM) normalized for height in M-2.7. The obese group displayed significantly higher LVMI and late mitral inflow velocity. Thirty-three obese subjects met the criteria for left ventricular hypertrophy (LVH) and had lower serum adiponectin levels compared with obese subjects without LVH and lean subjects (p<0.05). Adiponectin was negatively correlated with LVMI (R: -0.277, p: 0.002). Furthermore, during the partial correlation analysis where HOMA-IR was controlled, the negative correlation between adiponectin and LVMI progressed (r: -0.283, p: 0.002). The linear regression analysis showed an independent relationship between LVMI and adiponectin. (beta: -0.214, p: 0.01) Obesity is associated with LVH. This study showed direct influence of adiponectin on LVMI.Öğe In vitro antibacterial effects of topical local anesthetics(Taylor & Francis Ltd, 2008) Sedef Gocmen, J.; Buyukkocak, Unase; Caglayan, Osman; Aksoy, AltanBackground: The antibacterial activities of local anesthetics are recognized. Objective: To investigate in vitro the activity of topical local anesthetic ointments at clinical doses. Methods: The activity of two different local anesthetic ointments including lidocaine 5% and lidocaine/prilocaine 2.5% was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes and Enterococcus faecalis by the disc-diffusion method. Sterile discs containing topical local anesthetic drugs were prepared taking into account the doses of ointments used in clinical practice. The validity of the methodology was confirmed using topical antibacterial mupirocin. The inhibition zones of the discs were measured. Results: Mupirocin inhibited all the bacteria. Both local anesthetic ointments were found to be most effective on E. coli, whereas they had no effects on P. aeruginosa. Lidocaine 5% revealed antibacterial activity against S. aureus, S. epidermidis, E. coli, S. pyogenes and E. faecalis, but lidocaine/prilocaine 2.5% showed no activity on E. faecalis and inhibited S. pyogenes only at double doses. It was also observed that the antibacterial activity was in a dose-dependent manner. Conclusion: In the light of these findings, it might be concluded that topical local anesthetic ointments in routine settings may have a preventive role against some bacteria.Öğe In Vitro Investigation of the Antibacterial Effect of Ketamine(Taylor & Francis Ltd, 2008) Gocmen, Sedef; Buyukkocak, Unase; Caglayan, OsmanBackground: Antibacterial activity of local anesthetics especially lidocaine has been shown previously. In this study, the antibacterial effect of ketamine, a general anesthetic agent was investigated. Methods: The antibacterial effect of ketamine was studied using six different strains of bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Entecoccus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli) with disc diffusion method. Ciprofloxacine discs (CIP, oxoid) were used as a control to verify the methodology. Minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) of ketamine for these bacteria were also determined. Results: No inhibition was evident in discs containing 62.5 mu g of ketamine. Ketamine 125 mu g showed activity on all the bacteria tested with the exception of E. coli. The inhibition rates of Ketamine were more prominent at the doses of 250 mu g and 500 mu g similar to the inhibition rate of CIP. Whereas MIC and MBC values of ketamine for S. aureus and S. pyogenes were 500 mu g mL(-1), MIC and MBC values for P. aeruginosa were above 2000 mu g mL(-1). For other bacteria, these values ranged between these levels. Conclusions: Ketamine with higher doses showed antibacterial activity. We thought that it will be proper to use ketamine hesitantly in experimental animal studies like sepsis and translocation.Öğe Increased tumor necrosis factor alpha (TNF-α) and interleukin 1 alpha (IL1-α) levels in the lesional skin of patients with nonsegmental vitiligo(Blackwell Publishing, 2006) Birol, Ahu; Kisa, Ucler; Kurtipek, Gulcan Saylam; Kara, Fatma; Kocak, Mukadder; Erkek, Emel; Caglayan, Osman…Öğe Oxidative and nitrosative stress in patients with ischemic stroke(Walter De Gruyter Gmbh, 2018) Dogan, Ozlem; Kisa, Ucler; Erdemoglu, Ali Kemal; Kacmaz, Murat; Caglayan, Osman; Kurku, HuseyinBackground: Oxidative and nitrosative stress is well believed to play a role in the pathogenesis of ischemic stroke. This study aims to evaluate the time course of oxidative and nitrosative stress in ischemic stroke. Methods: In total, 27 healthy individuals, 22 individuals with high risk of ischemic stroke due to hypertension and diabetes mellitus, and 20 patients with acute ischemic stroke hospitalized at the Neurology Department of the Kirikkale University School of Medicine were enrolled in the study. Venous blood was collected at admission (hour 0) and again at hours 24, 48, 72, and 96. Nitric oxide (NO), malondialdehyde (MDA), total oxidative stress (TOS), oxidative stress index (OSI), and total antioxidant status (TAS) were measured and compared among stroke patients and control groups. Results: Blood NO was significantly higher in the patient group at 0, 24, 48, and 72 h compared to the healthy and high-risk control groups, and lower at 96 h than at early times within the patient group (p<0.001). MDA was higher in patients than the healthy control group at all times. Conversely, TOS and OSI were significantly lower in the patient group than the healthy control group at 96 h and the high-risk control group at 72 and 96 h (p <0.05). There was a significant correlation between initial NO (0 h) and duration of hospitalization (r= 0.71; p= 0.0003). Conclusions: These findings suggest a substantial early increase in oxidative and nitrosative stress in ischemic stroke patients during the first 2 days post-admission. However, TOS was lower by days 3-4, likely due to pathological recovery and local/systemic defense systems. The correlation between elevated serum NO during the acute phase of stroke and duration of hospitalization suggests NO as a potentially valuable predictor of ensuing oxidative damage and clinical outcome.Öğe Prevalence of coeliac disease in patients with autoimmune thyroiditis in a Turkish population(W J G Press, 2007) Güliter, Sefa; Yakaryilmaz, Fahri; Özkurt, Zübeyde; Ersoy, Reyhan; Ucardag, Derya; Caglayan, Osman; Atasoy, PinarAIM: To investigate the prevalence of coeliac disease in a series of Turkish patients with autoimmune thyroiditis. METHODS: Sera from 136 consecutive patients with newly diagnosed autoimmune thyroiditis and 119 healthy blood donors were tested for IgA tissue transglutaminase antibody with enzyme-linked immunosorbent assay. Endoscopic mucosal biopsy from the second part of duodenum was performed in patients with positive antibody test. RESULTS: Eight patients (5.9%) and one control subject (0.8%) were positive for IgA tissue transglutaminase antibody (OR: 7.38, 95% CI: 0,91-59.85, P = 0.04). Six patients and one control agreed to take biopsies. Histopathological examination revealed changes classified as Marsh IIIa in one, Marsh II in one, Marsh I in two, and Marsh 0 in two patients with autoimmune throiditis, and Marsh I in one blood donor. CONCLUSION: Turkish patients with autoimmune thyroiditis have an increased risk of coeliac disease and serological screening may be useful for early detection of coeliac disease in these patients. Our findings need to be confirmed in a larger series of patients. (c) 2007 The WJG Press. All rights reserved.Öğe Prevention of Retrosternal Pericardial Adhesions After Cardiac Surgery With Mitomycin C(Elsevier Science Inc, 2014) Orhan, Atilla; Gormus, Niyazi; Toy, Hatice; Gormus, Isik Solak; Caglayan, Osman; Tanyeli, OmerBackground Cross Mark Retrosternal pericardial adhesions may cause significant injuries to the heart and great vessels in cases that require reoperation. Fibroblast proliferation is one of the mechanisms for adhesion formation. Inhibition of fibroblast proliferation is important in reduction of retrosternal adhesions. Mitomycin C (MMC) is able to reduce fibroblast proliferation. We aimed to determine the effect of MMC on prevention of retrosternal pericardial adhesions after primary cardiac operations. Methods Thirty Sprague-Dawley female rats were used in the study. The rats were divided into two groups. First group was control (n = 10) and second group was MMC study group (n = 20). Xiphoid cartilage resection and retrosternal abrasion via subxiphoidal incision was performed. In the study group, MMC (1 mg/kg) was topically applied to the retrosternal space. After 15 days, rats were taken into reoperation and adhesion was graded. Tissue and blood samples were taken before termination procedure. Standard staining procedures and fibroblast growth factor receptor 3 antibody staining, were applied immunohistochemically to tissue samples. Results The average adhesion scores of the control (n = 10) and study (n = 20) (MMC; 1 mg/kg) groups were 2.50 +/- 1.27 and 0.70 +/- 0.86, respectively. The adhesion score of the study group was lower than the control group (p < 0.05). Immunohistochemical samples revealed that tissue fibroblast intensity was significantly higher in the control group than the study group (p < 0.05). There was no statistical significance between two groups in case of hydroxyproline levels (p > 0.05). Conclusion MMC was found to be effective in the prevention of retrosternal pericardial adhesions without any delay in normal tissue regeneration.
