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Öğe Characterisation of coagulase positive Staphylococcus species isolated from bovine mastitis using protein and plasmid patterns(Scientific Technical Research Council Turkey-Tubitak, 2009) Arslan, Emine; Celebi, Ayten; Acik, Leyla; Ucan, Uckun SaitThe objective of this work was to study the protein patterns, plasmid profiles, and antibiotic susceptibility of Staphylococcus intermedius and Staphylococcus aureus isolates originating from mastitic mammary glands of dairy cattle in different parts of Konya province. A total of 114 Staphylococcus species were isolated and identified by conventional bacteriological methods from bovine mastitis. Of the total isolates 77 were identified as S. aureus and 37 as S. intermedius. Intra- and inter-species diversities in the coagulase-positive staphylococci were investigated by analysis of whole-cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Plasmid profiling also demonstrated that 75 S. aureus isolates and 36 S. intermedius isolates contained plasmid. In addition, 88.3% of S. aureus and 59.4% of S. intermedius isolates were resistant to penicillin. Sixty-six of the 77 S. aureus isolates were also resistant to amoxicillin+clavulanic acid (85.7%). The corresponding number for S. intermedius was 17 (45.9%). Only 1 S. aureus isolate was resistant to danofloxacin. One of each of the Staphylococcus isolates was resistant to methicillin. Results from the study showed that the susceptibility of S. intermedius isolates to antibiotics used widely in mastitis therapy is a matter of concern.Öğe Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples(Tubitak Scientific & Technical Research Council Turkey, 2014) Togay, Sine Ozmen; Temiz, Ayhan; Celebi, Ayten; Acik, Leyla; Yalcin, Siddika SongulEnterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg(2), gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.Öğe Taxonomic Relationships in Turkish Verbascum L. Group A (Scrophulariaceae): Evidence from SDS-PAGE of Seed Proteins and a Numerical Taxonomic Study(Walter De Gruyter Gmbh, 2009) Celebi, Ayten; Karaveliogullari, Faik Ahmet; Acik, Leyla; Celep, FerhatObjective: The aim of this study is to clarify the taxonomic relationships of Turkish Verbascum species in Group A by means of seed protein profiles and numerical taxonomic study. Methods: Cluster analysis was carried out by UPGMA on the Nei genetic similarity coefficient using seed protein profiles. A numerical taxonomic study has been conducted on taxonomically problematic species such as V. brachysepalum, V. orientale, V. flabellifolium, V. trapifolium, V. dudleyanum and V. pyroliforme in order to elucidate their taxonomic relationships. For this reason, 14 OTUs (Operational Taxonomic Units) were investigated and scored on the basis of 20 character states by means of using MVSP software. Results: In this study, all Verbascum species in the Group A in Turkey were investigated by means of morphological, SDS-PAGE and a numerical taxonomic method. The total number of protein band positions observed for each species between 5 and 13. The most similar band pattern was observed in the region of 18 kD-14.4 kD. One band around 14 kD observed among all species studied and an 18 kD were found most of the species. Genetic distances among species were ranged between 0% and 58%. The results of the numerical taxonomic study are supported both morphologic and SDS-pAGE analysis. Conclusion: Depending on the seed protein profiles, numerical taksonomy and morphological characters, our results clarified the taxonomy of Turkish Verbascum (Group A) species. The results of the studies were in broad agreement to the arrangement of these species in Revision of the Genus Verbascum L. (Group A) in Turkey. As well as, their expanded and amplified morphological characters were given for the first time.Öğe Two new species of Cephalaria (Caprifoliaceae) from Turkey(Tubitak Scientific & Technical Research Council Turkey, 2012) Gokturk, Ramazan Suleyman; Sumbul, Huseyin; Celebi, Ayten; Acik, LeylaTwo new Cephalaria Schrad. ex Roem. et Schutt. species (Caprifoliaceae) from Turkey, Cephalaria davisiana Gokturk & Sumbul and Cephalaria sumbuliana Gokturk, are described and illustrated. A map showing their distribution and other related taxa is given. The morphological differences of the 2 new species are discussed and comparisons are made with the similar Cephalaria speciosa Boiss. & Kotschy and Cephalaria elmaliensis Hub.-Mor. & V.A.Matthews. In addition to morphological observations, C. davisiana, C. sumbuliana, C. elmaliensis, and C. speciosa were analysed by applying RAPD-PCR techniques. RAPD methods clearly distinguished between the new species and the 2 species that they resemble.
