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    Detection and isolation of Leishmania infantum from natural infected dog in Türkiye
    (Elsevier, 2025) Damlapinar, Aysegul; Berberoglu, Umut; Mungan, Mesut; Celebi, Bekir; Gazyagci, Aycan N.; Yildiz, Kader
    Some clinical signs such as cachexia, alopecia, exfoliative dermatitis, hair loss, and swollen lymph nodes were observed in a one-year-old crossbred male dog living in a rural area of Kirikkale province, T & uuml;rkiye, was presented to the veterinary clinic by its owner. Anaemia, leucopenia, hyperglobulinemia, and bilirubinemia were detected. Seropositivity was detected using Leishmania IgG/IgM Rapid Test. The amastigote forms of the parasite were observed in the lymph node aspirates. Viable promastigotes were observed in the samples obtained from the Novy-MacNeal-Nicolle medium (NNN) tubes. The parasite DNA was extracted from the promastigotes produced in the NNN medium using a DNA extraction kit according to the manufacturer's instructions. The specific bands indicating the gene regions of L. infantum (350 bp and 730 bp for HSP20 and HSP70, respectively) were observed. Additionally, BLASTn analysis revealed 100 % similarity with GenBank-deposited L. infantum sequences. The sequences were submitted to GenBank (Accession numbers: OR806945 and OR806946). According to the author's knowledge, it is the first dog isolate deposited in the collection of the Microbiology Reference Laboratories and Biological Products Department, General Directorate of Public Health, Ministry of Health, Ankara, T & uuml;rkiye. The isolate (TR_L. infantum CanL-1_damla) has been preserved by cryopreservation at -150 degrees C for further studies.
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    Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: Special emphasis to serologic co-existence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes
    (Elsevier Science Bv, 2009) Yildiz, Kader; Kul, Oguz; Babur, Cahit; Kılıç, Selçuk; Gazyağci, Aycan N.; Celebi, Bekir; Gurcan, I. Safa
    The aim of this study was to determine seroprevalence of Neospora caninum in dairy cattle ranches with higher abortion rates and co-existence of Neospora caninum, Toxoplasma gondii, Brucella abortus, and Listeria monocytogenes antibodies. The blood samples were collected from dairy cows with history of abortion (n = 234) as well as from pregnant cows that were (n = 323) kept in the same ranches. N. caninum seroprevalence was 10.77% (60/557). The co-existence rate of N. caninum seropositivity with T. gondii, B. abortus and L. monocytagenes was 24.77% (138/557), 13.82% (77/557) and 42.85% (162/378), respectively. Only one animal had significant antibody titers for all analyzed infectious agents. The seroprevalence of N. caninum and T. gondii were significantly higher in pregnant cows than aborted cows (p < 0.05 and p < 0.001, respectively). As a result, the comprehensive data generated through this study can significantly contribute to understanding of serologic association of N. caninum with T gondii, B. abortus and L monocytogenes in pregnant and aborted dairy cows. (C) 2009 Elsevier B.V. All rights reserved.