Yazar "Cinar, Mehmet Ulas" seçeneğine göre listele

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    Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits
    (Elsevier Sci Ltd, 2013) Kayan, Autchara; Uddin, Muhammad Jasim; Kocamis, Hakan; Tesfaye, Dawit; Looft, Christian; Tholen, Ernst; Cinar, Mehmet Ulas
    The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F-2 Duroc x Pietrain (DuPi, n = 313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n = 110) population. HNF1A mRNA and protein expressions were higher (p < 0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p > 0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect. (C) 2013 Elsevier Ltd. All rights reserved.
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    Association Of Number Of Artificial Inseminations Per Pregnancy In Holstein Dairy Cows With Polymorphism In Luteinizing Hormone Receptor And Follicle Stimulating Hormone Receptor Genes
    (Univ Ljubljana, Veterinary Faculty, 2017) Arslan, Korhan; Akyuz, Bilal; Akcay, Aytac; Ilgar, Esma Gamze; Macun, Hasan Ceyhun; Cinar, Mehmet Ulas
    Failure to become pregnant is the primary reason for a dairy cow to be culled from the production herd. A cow that is cycling normally, with no reproductive abnormalities, but has failed to conceive after at least three successive inseminations may cause economic losses in dairy farms. The present study aimed to examine the association between follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHCGR) genes polymorphisms and number of artificial inseminations in the Holstein cattle breed, raised in Turkey. A total of 264 Holstein cows were included in this study, consisting of 222 cows which had undergone a low number of artificial inseminations (two or less inseminations; LI) and 42 cows with a high number of artificial inseminations (three or more inseminations; HI). The polymerase chain reaction followed by restriction fragment length polymorphism method was used to determine the FSHR-AluI and LHCGR-HhaI DNA variants. Three genotypes (CC, CG and GG) were observed for the FSHR gene in LI and HI cows. No statistical difference was found among LI and HI animals for the FSHR genotypes (P=0.934). However, only the CC genotype was detected in LI cows whereas the CC, CT and TT genotypes were detected in HI cows for the LHCGR gene. The genotype frequency of CC was found to be highest (93%) in the HI animals and an association between LHCGR genotypes and the number of artificial inseminations per pregnancy was identified (P<0.001). This is the first report to describes an association between FSHR and LHCGR polymorphisms and number of artificial inseminations in cows.
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    Determining Gene Expression Profile of GPX 1 in the Liver of Diabetic Rats Treated with Capsaicin by Real-Time PCR
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Kurum, Aytul; Kocamis, Hakan; Deprem, Turgay; Cinar, Mehmet Ulas
    The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis.