Yazar "Cosan, D. Turgut" seçeneğine göre listele

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    Efficacy of butterbur in allergic rhinitis: a cell culture study
    (Verduci Publisher, 2023) Coskun, Z. Ozergin; Muluk, N. Bayar; Cosan, D. Turgut; Cingi, C.
    OBJECTIVE: The study aims to define butterbur's impact on nasal cells' viability and proliferation. After topically administering butterbur to the nasal epithelial cells, research has been done to see if butterbur has any harmful effect on the nasal cells. MATERIALS AND METHODS: Specimens of healthy primary nasal epithelium were collected from the subjects and incubated in cell culture in due course of septoplasty. After implementing 2.5 mu M butterbur in cultured cells, cell viability was defined via trypan blue assay, and proliferation was defined via the XTT method. The number of total cells, viability, and proliferation was defined. XTT (2, 3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide) experiments can be used to evaluate cellular toxicity. RESULTS: The findings of the XTT experiment reveal no harm to nasal cells after topical implementation of butterbur. No significant change in the proliferation of the cells, no matter what the doses are. There was no cytotoxic effect on the primary nasal cells at the end of 24 hours of implementation, and no side effects were found. There was no difference in cells' viability between the experimental group with butterbur application and the control group. CONCLUSIONS: Cytotoxicity on nasal cells was not observed after the butterbur application. Even if there have been some indications of liver toxicity, butterbur can be suggested as a safe option for seasonal allergic rhinitis. Further studies related to the toxicity of topical butterbur are also recommended, even though this study indicates no cytotoxicity from the topical application on nasal cells.
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    Investigation of the effect of the curcumin component as an alternative to the local treatment of nasal diseases
    (Verduci Publisher, 2023) Ceylan, E.; Cosan, D. Turgut; Muluk, N. Bayar; Cingi, C.
    OBJECTIVE: This study aimed to define the impacts of curcumin on nasal cell viability and proliferation. MATERIALS AND METHODS: Specimens of healthy primary nasal epithelium were collected and incubated in cell culture during septorhinoplasty from people who signed a consent form. After implementing 2.5 mu M curcumin in cultured cells, cell viability was defined via trypan blue assay, and proliferation was defined via the XTT method. The number of total cells, viability, and proliferation was defined. XTT (2,3-bis-(2-methoxy- 4-nitro- 5- sulphophenyl)-2H- tetrazolium-5-carboxanilide) experiments can be used to evaluate cellular toxicity. RESULTS: The results revealed no harm to nasal cells after the topical implementation of curcumin. There was no significant change in the proliferation of the cells related to 24 hours of implementation. There was no adverse effect of using curcumin on the cell viability, either. CONCLUSIONS: No cytotoxic effect on nasal cells has been observed after applying topically implemented curcumin. Curcumin could be used topically for an alternative treatment for allergic rhinitis as it has anti- inflammatory and immune response modulatory effects if clinical trials will confirm experimental data.
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    Is tannic acid a promising option in local treatment of nasal diseases?
    (Verduci Publisher, 2023) Alqunaee, M.; Muluk, N. Bayar; Cosan, D. Turgut; Cingi, C.
    OBJECTIVE: We investigated the effects of tannic acid on viability and proliferation of nasal cells after topical application. It was also evaluated whether tannic acid served as an alternative treatment agent. MATERIALS AND METHODS: Collected primary nasal epithelium from healthy people who had undergone septoplasty operations were incubated in cell culture. Following the implementation of 2.5 mu M tannic acid in cultured cells, both the number of total cells and their viability were measured using the trypan blue assay, while proliferation was assessed through the XTT method. The XTT method, which involves using 2,3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide, is a reliable means of determining cellular toxicity. RESULTS: XTT experiment results showed that there was no harm was detected to nasal cells after tannic acid's topical implementation. There were no significant changes in cell proliferation; moreover, no matter what the doses were. Additionally, no cytotoxic effects were detected on nasal cells primary culture at the end of the 24 hours of implementation. There was no side effect of it, either. CONCLUSIONS: According to the research, the application of tannic acid topically did not result in any harmful effects on the nasal cell culture. Tannic acid's potential anti-inflammatory properties and its ability to decrease Th2-related cytokines suggest that it may be beneficial for patients with rhinosinusitis or allergic rhinitis, pending confirmation through clinical trials. Additionally, if clinical trials confirm its effectiveness, tannic acid may be useful in healing wounds for patients undergoing septorhinoplasty.