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  • Küçük Resim
    Öğe
    Determining Gene Expression Profile of GPX 1 in the Liver of Diabetic Rats Treated with Capsaicin by Real-Time PCR
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Kurum, Aytul; Kocamis, Hakan; Deprem, Turgay; Cinar, Mehmet Ulas
    The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis.
  • Küçük Resim
    Öğe
    Immunohistochemical expression of anti-oxidants in bovine oviduct epithelial cells of estral and luteal phases
    (2016) Kürüm, Aytül; Deprem, Turgay; Kocamış, Hakan; Karahan, Siyami
    Summary: The present study aimed to evaluate immunohistochemical distributions of anti-oxidative enzymes Cu Zn-Superoxide dismutase (SOD-1), catalase, and Glutation peroxidase-1 (GPX1) in the bovine oviduct epithelial cells of estral and luteal phases. The results indicated both ciliated and secretoric cells of the oviduct mucosa exibited varying degrees of immureactivity for all. The SOD-1 and GPX1 immunostainings were more conspicuous in luteal phase while catalase immunostaining was more apparent in estral phase, especially in the isthmus region of the oviduct. In contrast to catalase, GPX1 immunoractivity was absent or limited in the isthmus. All regions of the oviduct mucosa had similar SOD-1 immunoreactivity. SOD-1 and GPX1 immunoreactivities were more apparent in samples of the luteal phase while catalase immnureactivity was higher in those of the estral phase. Presence of anti-oxidative enzymes catalase, SOD, and GPX1 immunostainings in the bovine oviduct suggests that the bovine oviduct epithelial cells are most likely engaged into synthesis of such enzymes and possibly the source of anti-oxidative enzymes in oviduct fluid. The oviduct regions, each of which executes different reproductive functions, varied by means of catalase and GPX1 expressions, suggeting that anti-oxidants may possibly contribute to different physiological proceses in the reproductive cycle. Furthermore, anti-oxidant expressions also varied between luteal and estral phases, suggesting that oviduct epithelial cells are possibly influenced by hormonal changes in regard to anti-oxidant expression. Presence of SOD-1 immunoreactivity in some but not all basal cells of the oviduct epithelial lining should be further investigated for possible heterogeneities among basal cells and for origin of secretory and ciliated cells.