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    Effects of Thalidomide and rhGM-CSF on Carbohydrate Metabolism in HL-60 Acute Promyelocytic Leukemia Cell Line
    (Akad Doktorlar Yayinevi, 2011) Boyunaga, Hakan; Dikmen, Gunnur; Ural, A. Ugur; Melekoglu, Abdullah
    The purpose of this study is to investigate the in vitro effect of thalidomide and rhGM-CSF on energy metabolism rate in HL-60 cell lines. HL-60 cells were incubated with radiolabelled glucose for 4 hours. Following incubation, radiolabelled CO2 was isolated and collected in specially designed scintillation vials. The other end-products of carbohydrate catabolism collected via anion-exchange chromatography were analyzed using liquid scintillation. Protein and glycogen levels for each group were also determined. The study results indicated that the use of drugs for 2-days had no significant effect when compared with aerobic and anaerobic controls. However, groups treated with the drugs for 5 days showed significant differences. It was obvious that rhGM-CSF caused HL-60 leukemia cells to use aerobic glycolytic pathway for production of energy. When thalidomide and rhGM-CSF combination therapy results were compared with untreated control cells, it was observed that glycogen consumption was decreased by 50.37%, and CO2 production was increased by 94.03%. When compared with those of anaerobic controls, glycogen consumption and CO2 production rates were found to be decreased by 54.01% and increased by 96.59%, respectively. As a consequence, we found that those cell lines treated with combined use of Thalidomide and rhGM-CSF caused HL-60 leukemia cells to predominantly prefer aerobic glycolytic pathways for energy production.
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    Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study
    (Springer, 2008) Somuncu, Salih; Cakmak, Murat; Dikmen, Gunnur; Akman, Huelya; Kaya, Murat
    We aimed to detect the protective effect of trapidil in ischemia-reperfusion (IR) injury due to ovarian torsion and detorsion. Thirty-two pubertal New Zealand albino rabbits were used. Adnexal torsion was created by rotating the left adnexa including the tubal and ovarian vessels in a 360 clockwise direction. Adnexal detorsion was done by untwisting the adnexa. In the IR group, left oopherectomy was performed after 3 h of adnexal torsion and 3 h of adnexal detorsion. In the study group, a 3-h adnexal torsion was performed and trapidil was administered intraperitoneally as a single dose of 40 mg/kg, 1 h before detorsion. The left oopherectomy was performed after a 3-h adnexal detorsion. In the sham group, sham operation was performed followed by left oopherectomy. In the control group, normal ovarian tissue was evaluated. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels of ovarian tissue were determined for each group. The values of SOD and GSH-Px activities in the IR group were significantly decreased (P < 0.05). In addition, the MDA level was significantly higher in the IR group (P < 0.01). The trapidil-administered group showed significant increase in the levels of GSH-Px (P < 0.05), catalase (P < 0.05), SOD (P < 0.05), and decreased MDA levels (P < 0.05) compared to those in the IR group. The study has shown that trapidil treatment prevents ischemia induced oxidative damage in the ovarian tissues of rabbits.
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    Protective effect of selenium on intussusception-induced ischemia/reperfusion intestinal oxidative injury in rats
    (Tubitak Scientific & Technical Research Council Turkey, 2010) Akman, Hulya; Somuncu, Salih; Dikmen, Gunnur; Ayva, Sebnem; Soyer, Tutku; Dogan, Pakize; Cakmak, Murat
    Aim: To evaluate the protective effect of selenium on intestinal ischemia-reperfusion injury due to intussusception. Materials and methods: Forty Wistar albino rats were allocated to 4 equal groups (n = 10) After anesthetization, 2 cm of intestinal segment, 20 cm proximal to the ileocecal valve, was removed in the control group (CG) In the intussusception group (ING), intussusception was performed 20 cm proximal to the ileocecal valve by pushing the proximal intestinal segment distally with a stylet, and 2 cm of intestinal segment was removed 4 h later Intussusception was reduced and a 2 cm segment was removed after another 4 h period in the intussusception-reduction group (IRG). The selenium group (SG) received 0.2 mg kg(-1) of selenium in 10 mL of 0 9% NaCl solution intraperitoneally 20 min before reduction and segments were removed 411 thereafter Intestinal tissues were evaluated for histopathological grades and oxidative injury markers Results: Histopathologic evaluations among the 4 groups did not reveal a significant difference (P > 0 05) Although catalase activity was decreased in all groups, increased activity was found in SG compared with IRG (P < 0 05) GSH-Px and SOD activities were also found to have decreased in ING and IRG (P < 0 05) CG and SG had no difference in GSH-Px and SOD activities (P > 0 05) Malondialdehyde (MDA) activities did not differ between CG and SG, but the MDA level was Increased in ING and IRG when compared with CG (P < 0 05) Conclusion: Although selenium prevents oxidative injury by increasing the antioxidant enzyme activity in experimental intussusception, minim effects on histopathologic findings were not detected