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    Bazı Vitis vinifera genotiplerinin RAPD-PCR metodu ile genetik analizi ve IBA'nın bu genotiplerin köklenme başarısı üzerine etkisi
    (Kırıkkale Üniversitesi, 2011) Dirican, Onur; Arıca, Prof. Şükran Çakır
    Bu tez çalışmasında Vitis vinifera (asma)'nın önemli gen merkezlerinden biri olan İç Anadolu bölgesinin kültür formlarından olan Hasandede ve Kalecik karası çeşitlerinin sınırlı bir genetik varyasyona sahip olduğu için Anadolu'nun zengin asma biyoçeşitliliğinden yararlanılabilmesi maksadı ile bu çeşitlerin genetik benzerliklerinin RAPD-PCR tekniği kullanılarak belirlenmesi ve IBA (İndol Butirik Asit)'in, bu çeşitlerin köklenme başarısı üzerine bulguların tespit edilmesi hedeflendi.RAPD-PCR tekniği, Vitis sp. Moleküler belirleyiciler veritabanı kullanılarak belirlenen primerler ile Hasandede ve Kalecik Karası çeşitlerinden 25 farklı bireye uygulanmış, bireyler arasında ve çeşitler arasında 300-400bp büyüklüğünde bantlar elde edildi. Hasandede çeşidinden 4, Kalecik Karası çeşidinden 7 olmak üzere toplam 11 adet polimorfik lokus gözlendi. Bununla beraber IBA asit uygulanan çeşitlerin, farklı IBA derişimlerinde farklı gelişimler gösterdiği ve çeşitler arasında gelişim açısından farklılığın olduğu görüldü. IBA her iki çeşit açısından da kök gelişimini pozitif yönde etkilerken, Hasandede çeşidinde 6000ppm, Kalecik Karası çeşidinde ise 7500ppm derişimde optimum kök gelişimi gözlendi. Bu fark istatistiksel açıdan da ifade edilerek, çeşitler arasında karşılaştırma yapıldı.Anahtar kelimeler: Vitis vinifera, RAPD-PCR, IBA, Köklenme
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    Beyin tümöründe gst-t1, gst-m1 ve p53 proteinlerinin rolü
    (Kırıkkale Üniversitesi, 2022) Dirican, Onur; Oğuztüzün, Serpil
    Oldukça riskli ve önemli kanser türlerinden biri olan beyin kanserinde tümör baskılayıcı p53 ile detoksifikasyon mekanizmasında görev alan Glutatyon S-Transferaz (GST) enzim ailesinin üyelerinin rollerinin araştırılması amaçlanmıştır. Beyin tümörlü hasta dokularında mitakondriyal GST izozimlerinden GST Mü-1 (GSTM1), GST Teta-1 (GSTT1) ve p53'ün protein ekspresyonları ile yine bu izozimlerin moleküler düzeyde delesyon ve nokta mutasyon (SNP) analizleri yapılarak enzim düzeyindeki ekspresyon durumları karşılaştırmalı olarak değerlendirilmiştir. Bu veriler hastaların bazı demografik ve klinik verileri ile de istatistiksel olarak değerlendirilmiştir. Çalışmamızda 142 beyin tümörlü hastanın 83 (%58,45)'i erkek, 59 (41,55)'u kadın hastalardan oluşmakta olup, hastaların yaş ortalamaları 49,44±8,09 dur. İmmunohistokimyasal analizler sonucu, GST-T ekspresyonu tümörlü dokularda normal dokulara göre 9,46 kat artışa neden olurken, bu artışın tümörlü dokulara sahip hastalarda hayatta kalım oranının ise anlamlı derecede azaldığı gözlemlenmiştir. GST-T protein ekspresyonunda, 60 yaş üzeri hastalarda anlamlı derecede ekspresyon artışı gözlemlenirken, kemoterapi tedavisi bu enzim ekspresyonunun artışına neden olduğu da belirlenmiştir. Normal dokularda tümörlü dokulara oranla diğer parametrelerden bağımsız olarak daha fazla p53 protein ekspresyonuna rastlandı. Özellikle hastaların GST-M delesyon durumları anlamlı şekilde gen kaybına uğrayarak, önemli ölçüde "null" allen frekansı gözlemlenmiştir (p<0,005). GST-M gen bölgesinde beyin tümörlü hastalarda 66(%44,3) "null" allele rastlanırken, GST-T gen bölgesinde bu sayı 7 (%4,9) şeklinde gözlemlenmiştir. GSTM1 null allel frekansı kadınlarda erkeklere göre daha sık olduğu gözlemlenmiştir [OR: 1.640 (0.837-3.215)]. GSTM1 "null" allel frekansına radyoterapi alan hastalarda almayanlara göre daha sık olduğu belirlenmiştir [OR=1.076 (0.548-2.114)]. GSTT1 "null" allel sıklığı kadınlarda daha yüksek olduğu belirlenmiştir [OR=1.105 (0.238-5.130)]. GSTT1 "null" allel frekansına 60 yaşın altındaki hastalarda daha yüksek olarak gözlemlenmiştir [OR=1.364 (0.255-7.296)]. GSTT1 "null" allel frekansına ise alkol tüketicilerinde daha sık olarak rastlanmıştır [OR=1.564 (0.175-14.016)]. GSTT1 "null" alleli, radyoterapi alan hastalarda daha sık şekilde gözlemlenmiştir [OR=1.212 (0.261-5.631)]. GSTT1 "null" allel frekansı kemoterapi alan hastalarda daha sık gözlemlenmiştir [OR=1.413 (0.261-7.653)]. GSTT1 "null" genotipi yaşayan hastalarda daha az sıklıkta bulunmuştur [OR=0.704 (0.151-3.278]. Tümör supresor p53 geninin genotip dağılımı şu şekilde belirlenmiştir: Arg/Pro (Heterozigot) %58.3. Arg /Arg (Vahşi Tip) %25.8 ve Pro/Pro (Mutant Tip) %15,8 şeklinde bulunmuştur. Pro kodlayan allel sıklığı ise hasta grubunda 0.45, kontrol grubunda 0.37 olarak bulunmuştur. Hasta grubunda kontrol grubuna göre Arg/Pro, Arg/Arg'dan 1.767 kat daha yüksektir. Pro/Pro, kontrol grubuna göre, hasta grubunda Arg/Arg'dan 1.839 kat daha yüksekti. Hastaların kontrol grubuna göre p53 ekzon4 kodon72 (SNP) gen bölgesinde guanin/sitozin dönüşümünden kaynaklı, mutasyon bir miktar yüksek olmasına rağmen anlamlı bir fark bulunamamıştır. Önemli rollerine rağmen, henüz bu enzimlerin ve gen bölgelerinin, beyin tümörü arasındaki ilişki üzerine yapılmış özellikle Türkiye'de geniş kapsamlı çalışma yoktur. Çalışmanın bölgesel anlamda yapılan çalışmalar kapsamında önemli bir yere sahip olduğu göz önüne alındığında, orijinalite açısından özgün değerinin yüksek olduğu düşünülmektedir.
  • Küçük Resim
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    Describing the Expression Profiles of Glutathione S-Transferase Mu and Tumor Protein 53 in Brain Tumor Tissue
    (Erciyes Univ Sch Medicine, 2024) Dirican, Onur; Kaygın, Pınar; Sarıaltın, Sezen Yılmaz ; Yılmaz, Can; Şimşek, Gülçin; Oğuztüzün, Serpil; Çoban, Tülay
    Objective: This study aims to explore the expression profiles of the glutathione S-transferaseMu (GST-M) isozyme and tumor protein 53 (p53) in both healthy and tumorous brain tissues. The findings are compared with clinical features and lifestyle factors to identify potential associations or correlations. Materials and Methods: We retrospectively analyzed the medical records of 149 patients diagnosed with primary or metastatic intracranial tumors. The expression levels of GST-M and p53 proteins were assessed in healthy and tumorous brain tissues using immunohistochemical staining. We also evaluated the associated clinical features and lifestyle factors. Results: There was a significant difference in the expression levels of GST-M between tumorous and healthy brain tissues, with tumor tissues showing higher expression (p<0.0001). Conversely, robust p53 expression was absent in both normal (97.3%) and tumor (78.5%) tissues. Nevertheless, a significantly higher prevalence of samples with p53 expression was found in the tumor group (p<0.0001). No associations were found between expression levels and clinical features or lifestyle risk factors. Furthermore, GST-M and p53 expression did not impact postoperative survival rates. Conclusion: The findings indicate an elevated expression of GST-M in brain tumor tissues, suggesting a potential role for GST-M in brain tumorigenesis.
  • Küçük Resim
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    Glutathione S-transferase enzyme activity and protein expression in patients with recurrent tonsillitis and idiopathic tonsillar hypertrophy
    (Palacky Univ, Medical Fac, 2019) Aydin, Sedat; Demir, Mehmet Gokhan; Oguztuzun, Serpil; Kilic, Murat; Yilmaz, Can; Dirican, Onur
    Objectives. The palatine tonsil is a significant part of the secondary immune system. Tonsillitis and idiopathic tonsillar hypertrophy (ITH) are the most common pathologies of this component. Although there are studies on their pathogenesis, there is insufficient study of the role of antioxidant agents. Glutathione S-transferase (GST) isozymes contribute to the antioxidation reactions in the tissue via the glutathione pathway. The purpose in this study was to reveal the levels of the GST enzyme activity and protein expression of GSTP1 and GSTA1 isozymes in patients with tonsillitis and tonsil hypertrophy, and to investigate their role in the pathogenesis of these diseases. Materials and Methods. Sixteen patients with recurrent tonsillitis and 5 patients with ITH and were included in the study. Cytosolic extracts were prepared from post-tonsillectomy tissues of both patient groups and GST enzyme activities were measured. Results. The expression of GSTP1 was found to be significantly higher than GSTA1 in tissue samples of patients with ITH and recurrent tonsillitis (P<0.001). Increased GST activity and GSTP1 isozyme expression were shown in patients with recurrent tonsillitis compared to the idiopathic tonsillar hypertrophy study group.There was a positive correlation between the expressions of GSTP1 (P=0.040; r=0.47). Conclusion. Increased GST activity and GSTP1 isozymes were demonstrated histologically in the pathogenesis of ITH and recurrent tonsillitis. We believe that the data of changes in antioxidant capacity, obtained from studies with more extensive and larger samples, would support our findings.
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    Glutathione-S-Transferase And P53 Expressions In Human Malignant, Benign And Metastasis Ovarian Tumor Tissues
    (Wiley, 2019) Dirican, Onur; Kaygin, Pinar; Oguztuzun, Serpil; Erdem, Aysegul; Simsek, Gulcin Guler; Sarialtin, Sezen Yilmaz; Coban, Tulay
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    GSTM1, GSTP1, p53 as some probable predictors of prognosis in primary and metastatic epithelial ovarian cancer
    (2023) Özer, Gizem; Kaygın, Pınar; Dirican, Onur; Oğuztüzün, Serpil; Sarıaltın, Sezen Yılmaz; Şımşek, Gülçin Güler; Erdem, Ayşegül
    Objectives: Ovarian carcinomas are responsible for the death of more women than all other gynecologic malignancies in the Western world. Ovarian carcinomas are detected in an advanced stage of the disease in approximately 80% of the patients. Glutathione S-transferases (GSTs) are an important family involved in the detoxification of several xenobiotics. Thus, this mechanism protects tissues from the harmful effects of oxidative stress and chemical-induced damages. The expression of them may contribute to the characteristics of ovarian carcinoma as they can metabolise both exogenous and endogenous compounds, which are implicated in the development of ovarian cancer. Therefore, our aim was to determine the expressions of GST Mu 1 (GSTM1), GST Pi 1 (GSTP1), and also p53, which is a tumor suppressor gene, in benign and malign ovarian tumors and metastasis tissues. Methods: A total of the 99 patients with ovarian tumor enrolled in the study. Thirty-one of the tissues was benign tumor, 17 was malign tumor and 51 was metastasis. The immunohistochemical GSTM1, GSTP1, and p53 staining characteristics of these tissues were investigated. Results: The highest GSTM1, GSTP1, and p53 expression was noted in the malignant group followed by the metastasis group. GSTP1 expression was significantly higher in malignant tissues than benign ones (p = 0.015). No statistically significant difference was observed in the level of GSTM1 expression between groups (p = 0.524). p53 expression was significantly higher in the metastasis and malignant tissues than the benign ones (p < 0.001). Conclusions: The higher expressions of GSTP1 and p53 in malignant and metastasis tissues than benign ones indicate that these expressions could be important biomarkers in ovarian cancer development and progression. Further studies with more cases are required to confirm the results of our present study.
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    Immunocytochemical Evaluation of Glutathione-S-Transferase P1 Enzyme in Patients with Rheumatoid Arthritis
    (Wiley, 2019) Dirican, Onur; Kaygin, Pinar; Oguztuzun, Serpil; Simsek, Gulcin Guler; Hayat, Buket; Sarialtin, Sezen Yilmaz; Coban, Tulay
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    Immunohistochemical approach to obesity disease in terms of expression levels of glutathione s-transferase (sigma, zeta, theta) isozymes
    (2023) Davudov, Mahammad; Bulus, Hakan; Dirican, Onur; Kaygın, Pınar; Şımşek, Gülçin Güler; Sarıaltın, Sezen Yılmaz; Gürbüz, Fatıma Nurdan
    Objectives: Obesity is a complex multifactorial disease with recently increasing prevalence and incidence. Several studies have been conducted to explain the ethiology, pathophysiology, epidemiology, molecular and genetic mechanisms, and effective treatments of obesity. Glutathione S-transferase (GST) S1, GSTZ1, and GSTT1 are essential enzymes for oxidative stress and metabolism-related disorders. For this purpose, we aimed to reveal the role of GSTS1, GSTZ1, and GSTT1 in obesity. Methods: The gastric tissue samples were taken from the patients diagnosed with obesity who underwent bariatric surgery in Ankara Keçiören Training and Research Hospital General Surgery Clinic between 2017 and 2019. Immunostaining was performed on paraffin-embedded tissues to evaluate GSTS1, GSTZ1, and GSTT1 expressions. Laboratory data of the patients were recorded. All the results were analyzed statistically. Results: Weak GSTS1 expression was observed in 38.1% of tissues and moderate in 6.3%. 37.3% of the tissues presented weak GSTZ1 expression, and 11 (8.7%) displayed moderate. There were weak GSTT1 expressions in 7.1% of the tissues and moderate 0.8% of them. A positive and statistically significant correlation was observed between GSTS1 and GSTT1 expression levels ((r)=0.028, p = 0.010; p < 0.05). There were no significant differences between expression levels and gender, age, comorbidities, and medication usage (p > 0.05). Conclusions: GSTs, in particular GSTS1, GSTT1, and GSTZ1, might contribute to molecular mechanisms and the progression of obesity. In our study, GSTS1, GSTT1, and GSTZ1 were found to be moderately expressed in gastric tissues taken from obese patients. However, new studies using more samples and advanced techniques are needed to elucidate the relationship.
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    Investigation of Glutathione S-Transferase-Alpha and Glutathione S-Transferase-Pi Expression Levels in Spermophilus xanthoprymnus and Meriones tristrami in Terms of Living Conditions and Natural Habitat Differences in Kırıkkale Province
    (2023) Pamukoğlu, Nahit; Oğuztüzün, Serpil; Dirican, Onur; Sarıaltın, Sezen Yılmaz
    Glutathione S-transferase (GST) is a multifunctional enzyme that provides homeostasis by catalyzing the first step in the formation of the end product mercapturic acid in the detoxification metabolic pathway. Being found in mammals, insects, fish, birds, annelids, molluscs, and many microorganisms, GST takes part the elimination of toxic substances taken into body by consuming food, and their transport by binding non-substrate ligands (e.g. heme and bilirubin) with GSH. In addition, it can prevent reactive electrophilic compounds from harming the body by covalent bonding similar compounds to each other. These xenobiotic acceptors affected by GST include nitrogen halogen compounds, organophosphates, and polycyclic aromatic hydrocarbons. Xenobiotics are oxygenated by this enzyme system, the next mechanism of oxygenated products is more oxygenation, and these products become more easily soluble in water. In this study, Glutathione S-Transferase was detected in the liver tissue of Spermophilus xanthoprymnus and Meriones tristrami and its characteristic features were determined. For this purpose, the animals were anesthetized with sodium pentobarbital and their liver tissues were harvested. After necessary preparations were completed, the samples were analyzed by using immunohistochemical staining method and the expressions of GST isozymes were determined. As a result, glutathione s-transferase-alpha and glutathione s-transferase-pi expression levels were found to differ in Spermophilus xanthoprymnus and Meriones tristrami samples obtained from different localities of Kırıkkale province. Differences in GST enzyme expression in these species indicate that both species differ in their detoxification capacity and response to xenobiotics.
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    Investigation of GSTM1 and GSTT1 polymorphisms in obesity patients under bariatric surgery
    (Society of Pharmaceutical Sciences of Ankara (FABAD), 2021) Ünsal, Abdulkadir; Buluş, Hakan; Dirican, Onur; Oğuztüzün, Serpil; Öztürk, Doğan; Cihan, Mehmethan; Ada, Ahmet Oğuz
    Obesity is a chronic disorder with increasing prevalence worldwide and occurs when energy intake is more than energy expenditure. Obesity is one of the factors that cause oxidative stress and arises from an imbalance between the reactive oxygen species ROS and the cell’s antioxidant defense system. Increasing ROS in obesity, influencing the hypothalamic neurons, affect hunger and satiety control, so correspondingly on body weight control. When ROS amount increases, through DNA, protein, and lipid oxidation, cell damage, necrosis, and apoptosis take place. Oxidative stress increment in adipose tissue causes the development of metabolic syndrome in obese people. Also, weight loss due to calorie restriction or exercise reduces oxidative stress. Mitochondria is the essential source for ROS formation. In the electron transfer system, reactive oxygen species forming due to oxidative phosphorylation reactions are involved in various physiological processes such as cell proliferation and differentiation. Glutathione S-transferase M1 and T1 genes encode enzymes that have oxidant-scavenging activities. Deletion polymorphisms in these genes cause the absence of their corresponding enzymes. In this study, we investigated the parameters associated with obesity such as body mass index (BMI), TSH, glucose, satiety blood glucose, triglyceride, and cholesterol levels, and deletion polymorphisms of GSTM1 and GSTT1 genes in 152 patients diagnosed with obesity in a Turkish population. No statistically significant relationship was found between the parameters studied in obese patients and GSTM1 and GSTT1 polymorphisms. More studies are needed to elucidate the relationship of GSTM1 and GSTT1 polymorphisms with obesity. © 2021 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
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    Investigation of the role of CYP1A1 and CYP1B1 expressions in obesity susceptibility
    (2022) Polat, Fatih; Bulus, Hakan; Kaygın, Pınar; Dirican, Onur; Şımşek, Gülçin Güler; Sarıaltın, Sezen Yılmaz; Gurbuz, Nurdan
    Metabolik işleyiş, etkileri ve neden olduğu hastalıklar açısından daha multidisipliner bir bakış açısıyla anlaşılması gereken obezite, son yıllarda prevalansı ve insidansı artan hastalıklardan biri olarak görülmektedir. Bu hastalığın metabolik yolunda önemli enzim gruplarından biri olan sitokrom p450 (CYP1A1 ve CYP1B1) izozimlerinin rolünün ortaya konulması amaçlanmıştır. 2017-2019 yılları arasında Ankara Keçiören Eğitim ve Araştırma Hastanesi Genel Cerrahi Kliniği'nde obezite tanısı konulan ve bariatrik cerrahi, ksenobiyotik metabolizması uygulanan 152 hastaya immünohistokimya yöntemiyle CYP1A1 ve CYPB1 izoenzimlerinin ekspresyonu araştırılmıştır. CYP1A1 açısından elde edilen bulgular; 152 kişide CYP1A1 ve CYP1B1 immünohistokimya boyama düzeyleri incelenen dokuların %12.7'sinde CYP1A1 ekspresyonu gözlenmezken; %33.3, %32.5 ve %21.4'te zayıf bir CYP1A1 ifadesi gözlenmiştir. Dokuların %71.4'ünde CYP1B1 ekspresyonu görülmezken, dokuların %28.6'sında zayıf ekspresyon izlenmiştir. Hiçbir dokuda orta veya güçlü CYP1B1 ekspresyonu gözlenmemiştir. Kadın hastalardan alınan dokuların ortalama CYP1A1 ve CYP1B1 boyama seviyeleri erkek hastalardan daha yüksek bulunmuştur. Klinik verilerden diyabet parametresi (p
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    The Administration of Steroids and its Impact on Caspase-3 Expression in Pediatric Adenoid Hypertrophy
    (Springer India, 2024) Apaydin, Emre; Yasar, Buse; Simsek, Gulcin; Kaygin, Pinar; Sarialtin, Sezen Yilmaz; Dirican, Onur; Cetin, Hazal Eylem
    ObjectiveAdenoid hypertrophy is a prevalent pediatric condition, often necessitating surgical intervention. Intranasal steroid administration shows promise as a conservative treatment, particularly by inducing apoptosis in adenoidal cells, leading to a reduction in adenoid size and inflammation. This study aims to characterize the expression profile of caspase-3 as an apoptotic inducer protein in inflammatory and epithelial adenoid tissues and explore its association with steroid administration. MethodsWe performed immunohistochemical staining for caspase-3 proteins in adenoid tissues obtained from 51 pediatric patients aged between 2.5 and 12 years (mean age: 6.09 +/- 2.1 years) who underwent adenoid surgery. A retrospective analysis of clinical data was conducted, categorizing participants into steroid treatment receivers (n = 25) and non-receivers (n = 26). Subsequently, the lymphoid inflammatory tissue and epithelial tissue from the adenoid were compared in terms of caspase-3 protein expression, and associated clinical variables were assessed. ResultsImmunohistochemical analysis revealed significant caspase-3 expression in inflammatory tissues. The expression levels were scored, and no significant correlation was observed between inflammation and epithelium based on caspase-3 expression (correlation coefficient = 0.143; p > 0.05). Furthermore, demographic and clinical characteristics did not show a statistically significant difference in caspase-3 expression levels. ConclusionCaspase-3 expression was significant in inflammatory adenoid tissue, but it showed no association with nasal steroid administration.
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    The role of glutation-s-transferase mu1 and teta1 polymorphisms in chronic obstructive pulmonary disease
    (University of Ankara, 2021) Hayat, Buket; Yavuz, Muhsin Selçuk; Sahin, Engin; Dirican, Onur; Sarialtin, Sezen Yilmaz; Yilmaz, Can; Yildirim, Isil
    Objective: The aim of this study the investigation of "null" alleles in GSTM1 and GSTT1 gene regions in the development of COPD disease. Material and Method: There are 36 patients with COPD and 14 control cases, who received the Ethics Committee permission from Polatli Duatepe State Hospital Chest Diseases Department. DNA isolations were made from blood samples from the end of 2019 and the control group. Deletions in GSTM1 and GSTT1 gene regions were examined by qPCR method in COPD patient and control groups. The results of the study were evaluated comparatively by distributing the gene dose according to the Hardy-Weinberg. Result and Discussion: When seen from 36 COPD patients after qPCR analysis, it was found that deletion expressions due to gene doses in all patient groups; 23 individuals (63.8%) in the (+/-) genotype for GST-M1, 13 individuals (26.2%) in the (-/-) genotype. For GST-T1, 14 (%) in the (+/+) genotype 38.8), while deletion was observed with the highest rate, 4 individuals (11.1%) in the (+/-) genotype and 18 individuals (50.1%) in the (-/-) genotype were found. For GST-M1, deletion was observed in 19 individuals (63.3%) in the genotype (+/-) in male individuals, while it was observed in 4 individuals (66.6%) with the same genotype in women. While deletion was not observed in 11 (36.6%) male patients, this rate was observed as 2 (33.4%) in women. In the GST-T1 gene region, there were 10 (33.3%) males in male patients and 4 (66.6%) individuals in female patients with deletion occurring and the frequency of the "null allele" was high (+/+). In the (+/-) genotype, 3 (10%) in males and 1 (16.7%) in females were found. In the genotype where deletion was not observed and the gene was conserved (-/-), 17 (56.7%) individuals were observed in male patients and 1 (16.7%) in female patients. In the case that the gene "null" allele status in the GST-M1 gene region is slightly higher than the GST-T1 gene communication, this situation is thought to be a factor in obstructive pulmonary disease. © 2021 University of Ankara. All rights reserved.
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    Unveiling the etiological impact of GST-M1, GST-T1, and P53 genotypic variations on brain carcinogenesis
    (Springer Science and Business Media B.V., 2024) Dirican, Onur; Kaygın, Pınar; Oğuztüzün, Serpil; Husseini, Abbas Ali; Sarıaltın, Sezen Yılmaz; Yılmaz, Can; Ünlü, Nihan
    Background: Functional variants of glutathione-S-transferase (GST)-M1, GST-T1, p53 might modulate brain cancer risk by altering the rate of metabolism and clearance of carcinogens from the brain tissue. In this study, the role of GST-M1, GST-T1, p53 polymorphisms on brain tumor was investigated. Methods and results: Brain tumor tissues of 143 patients were obtained from the Gulhane Training and Research Hospital, Department of Neurosurgery between 2019 and 2020. In the xenobiotic mechanism, the null allele frequency in the GST-T1, GST-M1 gene regions of Phase II enzymes by qPCR method were investigated. Single nucleotide polymorphism encoding Arg/Pro conversion in the p53 gene region was analyzed in 120 cases by sequence analysis method. The data were analyzed statistically with patient’s demographic and clinical data. GST-M1, GST-T1, p53 genotypes of the patient group were determined. The most frequent genotype was null genotype (0/0) for GST-M1 (?2 = 39.756, p < 0.001). GST-M1 genotype frequencies were 30.8%, 23.1%, 44.3% for 1/1, 1/0, 0/0, respectively. The most frequent genotype was GST-T1 1/1 following by GST-T1 1/0 (?2 = 0.335, p = 0.846). GST-T1 genotype frequencies were 64.3%, 30.8%, 4.9% for 1/1, 1/0, 0/0, respectively. GST-M1 null genotype might be associated with the development of brain tumors. Genotype distribution obtained in p53 exon 4 codon 72; Arg/Arg was determined as 31 (25.8%), Arg/Pro 70 (58.3%), and Pro/Pro 19 (15.8%) in the case group, while there were 18 (38.3%), 23 (48.9%), and 6 (12.8%) respectively in the control group. However, the genotype distribution of p53 exon 4 codon 72 among tumorous tissue did not significantly vary from healthy control tissues (?²=2.536, p = 0.281). Conclusion: The null allele frequency encountered in the GST-M1, GST-T1 gene regions is consistent with the rates in the gene pool called Caucasian in the literature. GST-M1 gene polymorphism may play a crucial role in brain carcinogenesis in Turkish patients. This study based on clinical data is thought to help to understand the important epidemiological features of brain tumors. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.