Yazar "Duru, Mehmet Emin" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    A detailed study on multifaceted bioactivities of the extracts and isolated compounds from truffle Reddellomyces parvulosporus
    (Wiley, 2022) Çayan, Fatih; Tel-Çayan, Gülşen; Deveci, Ebru; Duru, Mehmet Emin; Türk, Mustafa
    Mushrooms and truffles are attracting attention as a new generation of biotherapeutics. In the current study, isolation, phenolic and organic acid composition, and antioxidant, cytotoxic, anticholinesterase activities of truffle Reddellomyces parvulosporus were examined. Four known compounds (brassicasterol (1), ergosterol peroxide (2), fumaric acid (3) and mannitol (4)) were isolated with the combination of chromatographic techniques. Fumaric acid (54.74 +/- 0.85 mu g g(-1)) was found as the major compound by HPLC-DAD. All isolated compounds were bioassayed for antioxidant, cytotoxic, anticholinesterase, anti-tyrosinase, anti-urease, anti-alpha-glucosidase and anti-alpha-amylase activities. Compound 1 indicated notable cytotoxic activity on MCF-7 (IC50: 38.08 +/- 0.75 mu g mL(-1)) and compound 3 on H1299 (IC50: 62.37 +/- 0.75 mu g mL(-1)). Also, compounds 1 (84.55 +/- 1.14%) and 2 (84.90 +/- 0.10%) showed higher anti-urease activity than thiourea (78.57 +/- 0.22%), while compound 2 (66.31 +/- 0.08%) displayed near-standard anti-BChE activity. Also, being the first to emphasise the potential of R. parvulosporus as a natural food additive, this study evidenced its medicinal importance by revealing bioactive compounds and properties.
  • [ X ]
    Öğe
    Chemical constituents and their bioactivities from truffle Hysterangium inflatum
    (Springer, 2021) Tas, Meltem; Kucukaydin, Selcuk; Tel-Cayan, Gulsen; Duru, Mehmet Emin; Ozturk, Mehmet; Turk, Mustafa
    Hysterangium inflatum is a truffle that grows naturally in the roots of Eucalyptus sp. and is distributed along the Mediterranean and Aegean coasts. Chemical investigation of the H. inflatum enabled to isolate of a new cerebroside, hysteroside (1), and seven known compounds namely, psyllic acid (2), brassicasterol (3), ergosterol (4), ergosterol d (5), ergosterol peroxide (6), ergosta-7,9,22-triene-3-O-beta-d-glucoside (7) and mannitol (8). IR, NMR, MS techniques were used for structural elucidation and supported with literature data. Antioxidant, cholinesterase, urease, and tyrosinase inhibitory and cytotoxic activities on MCF-7 breast, H-1299 lung cancer cells, and murine fibroblast (L929) non-cancerous cells of extracts and isolated compounds from H. inflatum were analyzed. All the isolated compounds, except compounds 2 and 8, displayed considerable cytotoxic activities against MCF-7 and H-1299 cancer cells. Compounds 1 (IC50: 18.11 mu g/mL) and 5 (IC50: 24.93 mu g/mL) were the most effective against MCF-7, while compounds 6 (IC50: 27.61 mu g/mL), 1 (IC50: 36.20 mu g/mL) and 5 (IC50: 38.62 mu g/mL) showed most potent toxicity against H-1299 and the extracts and compounds have no toxic effect on L929. Among the extracts, the methanol extract displayed the best antioxidant activity in all assays. Compound 1 exhibited highest enzyme inhibition activities with value of 58.71%, 52.84%, 45.37%, and 35.63%, against urease, tyrosinase, butrylcholinesterase (BChE), and acetylcholinesterase (AChE) enzymes at 100 mu g/mL concentration, among the isolated compounds. These results support that H. inflatum is a steroid-rich truffle and might be a potential source, especially for ergostane type steroids.