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    Öğe
    Bolu İlinde Bulunan Ticari ve Damızlık Broiler İşletmelerinde Antibakteriyel İlaçlar için Farmakovijilans Taraması
    (2015) Ekici, Hüsamettin; Dakman, Asiye; Ekici, Seda; Coşar, Mustafa; Güleç, Metin
    Bu çalışmada hazırlanan farmakovijilans anketinde bakteriyel hastalıkların ticari broiler ve damızlık broiler işletmelerinde görülme sıklığına, antibakteriyel ilaç kullanımından önce antibiyogram yaptırma ve bunların tedaviye yanıtverme durumu ile farmakovijilans bildirimi gibi bazı sorular yöneltilmiştir. Bu amaçla oluşturulan formlar Bolu ilinde ticaribroiler kanatlı sektöründe çalışan 110 adet veteriner hekim (10 adet ticari işletmede) ve 14 adet damızlık broiler sektöründe çalışan veteriner hekimlere (4 adet damızlık işletmede) doğrudan ulaştırılmıştır. Anket çalışması sonucuna göre;işletmelerin son bir yıl içerisinde %92.8'unda (13 adet işletmede) bakteriyel kaynaklı hastalıklar görüldüğü, hastalıkvakalarının %70.16'sında (87/124) antibiyogram yapılmadan antibakteriyel ilaç kullanıldığı, antibiyogram yapılmadanilaç kullanılması durumunda yaklaşık %58.6'sının bazen tedaviye yanıt aldığı, ilaç seçiminde ticari kanatlı sektöründeçalışan veteriner hekimlerin uygulanabilirliğe, damızlık işletmelerde ise edinilebilirliğe önem verdikleri saptanmıştır.
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    Öğe
    COVID-19 İÇİN MOLEKÜLER TANI YÖNTEMLERİNE GENEL BAKIŞ
    (2020) Semen, Zeynep; Ekici, Seda; Malas, Büşra
    Bu derleme, kısa bir süre içinde pandemiye sebep olan, yeni bir koronavirüs SARS-CoV-2’nin moleküler tanı yöntemleri ile ilgili bilgi vermek amacıyla hazırlanmıştır. Global olarak ciddi bir tahribata yol açan COVID-19 pandemisini önleyebilmek adına, hassas ve spesifik laboratuvar teşhis metotlarının geliştirilmesi, vakaların hızlı ve güvenilir şekilde tanımlanabilmesi için oldukça önemlidir. Diyagnostik testler COVID-19 pandemisinde önemli bir rol oynamıştır ve oynamaya devam edecektir. COVID-19 için şüphelenilen vaka tanımını karşılayan hastalardan hızla numune alınması ve test edilmesi klinik yönetim ve salgın kontrolü için bir önceliktir. Solunum sekresyonlarında SARS-CoV-2 saptama yeteneği, bireyin enfekte olma zamanını ve diğer kişilere bulaştırma potansiyelini belirlemek için gereklidir. Viral tespit, bireysel hastaların tanımlanması, pandemi yönetimi ve ayrıca virüsün bir topluluğa ne zaman bulaştığını ve ne kadar hızla yayıldığını belirlemek için kullanılır. Topluluklar, kapanma dönemlerinin ardından yeniden açılmaya çalıştıkça, hem SARS-CoV-2'nin hem de virüsü tanıyan spesifik antikorların saptanması, bireylerde ve topluluklarda enfeksiyon ve bağışıklığın değerlendirilmesi için bir araç olarak giderek daha önemli hale gelecektir. Bu amaçla, SARS-CoV-2 genomunu tespit etmeye yönelik nükleik asit amplifikasyon testleri ve serolojik testler kullanılmaktadır.
  • Küçük Resim
    Öğe
    Effects of different fissure sealant applications on laser fluorescence measurements
    (Wiley, 2011) Sonmez, Isil Saroglu; Oba, Aylin Akbay; Erkmen, Merve; Ekici, Seda
    Objective. The aim of this in vitro study was to evaluate the effects of using only phosphoric acid or a self-etch bonding agent under clear and opaque fissure sealants on laser fluorescence (LF) readings and the reproducibility of the laser device. Methods. Eighty extracted permanent molars, ranged from sound to carious, were randomly divided into four groups: phosphoric acid + opaque sealant (group I), Clearfil S3 Bond (Kuraray, Kurashiki, Japan) + opaque sealant (group II), phosphoric acid + clear sealant (group III), and Clearfil S3 Bond + clear sealant (group IV). The teeth were measured using an LFpen device, before and after sealing. Data were analysed using the Spearman's correlation, Wilcoxon signed rank, and Mann-Whitney U-test. Results. Except group IV, there was a statistically significant decrease in fluorescence after the application of sealants (P < 0.05). The decrease of LFpen readings in the opaque sealant groups was more significant than the clear sealant groups (P < 0.05). But for both sealants, the difference between phosphoric acid and Clearfil S3 Bond groups was nonsignificant (P > 0.05). Conclusions. There was a statistically significant decrease in fluorescence for both clear and opaque sealant groups. However, clear sealant with Clearfil S3 Bond does not influence the LFpen readings.
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    Öğe
    Investigation of immunomodulatory and cytotoxic effects of shed snake skin (Elaphe sauromates) extract
    (Frontiers Media Sa, 2024) Sinmez, Cagri Caglar; Tufekci, Emre; Demir, Busra Seniz; Eken, Ahmet; Gunes, Vehbi; Ekici, Seda; Bozkaya, Esra
    Introduction: Shed snake skin (SSS) is commonly used empirically in ethnomedicine to treat psoriasis, acne, warts, eczema, scabies, open wounds, hemorrhoids, and glaucoma. Although a few studies exist, SSS extracts' in vitro immunological effects have yet to be well described. Therefore, we aimed to investigate the immunomodulatory effects of SSS extract on murine lymphocytes and T cells. Methods: Hexane, methanol, and chloroform extractions were conducted in collected SSS samples. Protein concentrations in the SSS extract were measured. The cytotoxic and anticancer activities were measured using L929 Fibroblast and SK MEL 30 Cell Lines via MTT assay as described in TS EN ISO 10993-5. Immunomodulatory activities of SSS extract on total lymphocytes or enriched CD4(+) T cell cultures, their cell-specific pro-inflammatory cytokines (IL-6, IL-1 beta. IL-12p40, IL-23p19, TNF-alpha, IL-17A, IFN-gamma, IL-10, TGF beta 1) levels were measured via FACS ARIA III analysis and related gene expression with Real-Time Quantitative Polymerase Chain Reaction (Rt-qPCR). Results: Hexane, methanol, and chloroform-extracted SSS were tested on SK-MEL-30 cells via MTT and revealed a superior anti-proliferative effect for hexane extract of SSS at low concentrations. SSS treatment of murine lymphocytes augmented Tnf-alpha and IFN-gamma levels produced by CD3(+) T cells when lymphocytes were activated with anti-CD3/CD28 or LPS stimulation. This effect required the presence of non-T cells, possibly antigen-presenting cells, and was not observed on purified CD4(+) T cells. Additionally, SSS significantly blocked suppressive cytokine Tgfb gene expression (but not Il10) without altering in vitro Treg generation/or expansion. Discussion: This is the first in vitro study investigating SSS's anti-tumor and immunomodulatory effects. Our data provide evidence for SSS's anti-proliferative activity on SK-MEL-30 cells and its pro-inflammatory role on murine lymphocytes, which warrants further investigation of the potential use of SSS extract with in vivo disease models.
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    Öğe
    Investigation of the biological activity and toxicity of bioactive silver nanoparticles synthesized via Vitex agnus-castus seed extract on honey bees
    (Springer, 2024) Ekici, Seda; Bozkaya, Ogun; Sevin, Sedat; Erdem, Babur; Arslan, Okan Can; Cinar, Ozge Ozgenc; Bozkaya, Esra
    In this study, silver nanoparticles (AgNPs) coated with bioactive molecules were synthesized via Vitex agnus-castus L. (VAC) seed extract (VAC/AgNPs). The synthesized VAC/AgNPs were characterized by surface plasmon resonance (SPR) ultraviolet-visible region spectroscopy (UV-Vis). The hydrodynamic size and surface charge analysis of the particles were measured with a Zeta sizer. The results of UV-Vis and Zeta analysis revealed that AgNPs were synthesized, the size distribution was nanoscale, and the solution was stable. The effects of the synthesized VAC/AgNPs and aqueous extract of VAC seeds on honeybees were investigated by means of lifespan test and histopathological analysis. It was determined that both VAC seed extract and VAC/AgNPs were non-toxic to honeybees at certain doses, positively affected their life span and contributed to their longevity in the life span test. Furthermore, no adverse effects were detected in terms of intestinal health in histopathological examinations. Therefore, VAC/AgNPs are considered to be a promising bioactive agent for honeybees.
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    Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract
    (Ankara Univ, 2023) Bozkaya, Ogun; Ekici, Husamettin; Gun Gok, Zehra; Arat, Esra; Ekici, Seda; Yigitoglu, Mustafa; Vargel, Ibrahim
    In recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FT-IR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CA-AgNPs at <= 1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CA-AgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.
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    Vitex Agnus-Castus L. Nanoparticles: Preparation, Characterization and Assessment of Antimicrobial and Anticancer Activity
    (Wiley-V C H Verlag Gmbh, 2023) Ekici, Seda; Bozkaya, Esra; Bozkaya, Ogun; Cerci, Nebahat Aytuna; Aluc, Yasar; Ekici, Husamettin
    This study was aimed to synthesise antimicrobial and therapeutic silver nanoparticles (AgNPs) by green synthesis method using Vitex agnus-castus L. (VAC) seed extract. The synthesised VAC seed extract coated AgNPs were characterised by UV-Vis spectrometry, Zeta analysis and FT-IR spectrometry. The antifungal, antimicrobial, cytotoxicity by MTT assay, and anticarcinogenic activities on HeLa cells line were investigated. VACAgNPs exhibited high antimicrobial activity against S. aureus with a zone diameter of 11.3 +/- 0.6 mm. In addition, the cytotoxicity of VAC-AgNPs was evaluated using the L929 fibroblast cell line and the HeLa cells line, with AgNPs applied at six different concentrations. At a concentration of 5.0 mM, viability was found to be 47.6%+/- 4.2% in L929 fibroblast cells and 39.6%+/- 7.9% in HeLa cells, suggesting a potential antiproliferative effect of VAC-AgNPs on HeLa cancer cells. The IC50 values of VAC-AgNPs against the HeLa cell line were calculated as 2.94 mu g/ml.