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    The identification of intestinal M cells in the sacculus rotundus and appendix of the Angora rabbit
    (Springer, 2010) Beyaz, Feyzullah; Ergun, E.; Bayraktaroglu, A. G.; Ergun, L.
    The present study was aimed at the immunohistochemical demonstration of M cells, found in the follicle-associated epithelium (FAE) of the sacculus rotundus (SR) and appendix of the Angora rabbit, using anti-vimentin primary antibodies, and at the determination of certain fine structural characteristics. Ten adult Angora rabbits constituted the material of the study. Immunohistochemical staining revealed that many cells composing the FAE, which covered the dome regions of the SR and appendix, reacted positively with vimentin. FAE contained two different types of vimentin-positive cells. The first type surrounded intraepithelial lymphocytes (IEL) with a basolateral invagination in the apex and periphery of the dome epithelium, whilst the second type consisted of columnar cells found in the FAE near crypts. The immunoreactivity of the cells found in the FAE covering the apex and periphery of the domes was observed particularly in the perinuclear cytoplasm and the cytoplasm surrounding the IEL. Electron microscopic examination demonstrated that the M cells found in the FAE covering the apex and periphery of the dome regions of the SR and appendix did not exhibit any microvilli on their apical surface. The FAE near crypts contained columnar cells, which resembled enterocytes. The apical membrane of these cells exhibited shorter and irregular microvilli, in contrast to neighbouring enterocytes. It was determined that M cells, found in the FAE of the SR and appendix in the Angora rabbit, displayed similarities in terms of localization and fine structure. This situation may be indicative of the two lymphoid structures with different localization having similar functional properties.
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    Immunohistochemical Expression of Nitric Oxide Synthase Enzymes (iNOS, eNOS, nNOS) in the Estrual and Luteal Phases of the Sexual Cycle in the Cow Oviduct
    (Wiley-Blackwell, 2013) Ozen, A.; Ergun, E.; Oztas, E.; Ergun, L.; Ozcan, Z.; Alabay, B.; Erdogan, E.
    This study was aimed to determine staining intensity, cellular localization and distribution of the nitric oxide synthase (NOS) enzymes during the sexual cycle in the cow oviduct. Oviduct samples belonging to 20 cows, 10 of which were in the estrual phase and 10 in the luteal phase of the sexual cycle, were examined by an immunohistochemical procedure to determine the presence of the NOS enzymes. In the epithelial cells of the isthmus, endothelial NOS (eNOS) expression showed a strong positive reaction during the estrual phase and a weak positive reaction during the luteal phase in the endothelium and smooth muscle of the blood vessels found in the serosa and lamina propria. eNOS expression was not observed in the epithelium of either the ampulla or the fimbria in the two particular phases of the sexual cycle. The eNOS reactions observed in the blood vessel wall in these regions were stronger during the estrual phase. eNOS activity was not observed in the tunica muscularis in any of the regions of the oviduct. During the estrual phase, it was observed that inducible NOS expression showed a stronger positive reaction in the epithelium and muscle layer of the isthmus and ampulla and in the epithelium of the fimbria, compared to the luteal phase. Neuronal NOS immunoreactivity was observed in the epithelial cells of all oviduct regions and in the muscle layer of the isthmus and ampulla and did not display any significant difference between the estrual and luteal phases.
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    Light and electron microscopic morphology of Paneth cells in the sheep small intestine
    (Ecole Nationale Veterinaire Toulouse, 2003) Ergün, E.; Ergun, L.; Asti, R.N.; Kürüm, A.
    The aim of the study was to describe the light and electron microscopic morphology of Paneth cells in the sheep small intestine, as well as their histochemical properties, location and numerical distribution. Samples from the small intestine: duodenum, jejunum and ileum, of 7 healthy adult sheep were studied. The Paneth cells are located in the crypts of Lieberkuhn and are more numerous in the base and neck of the crypts than in the higher parts. They are characterised by a basal nucleus, and acidophilic apical granules. Granules are larger in Paneth cells located in the deep portions of crypts. The numerical distribution of Paneth cells is heterogeneous along the small intestine, with higher density in the jejunum, and decreasing densities in the duodenum and ileum. The difference between the three regions of the small intestine is statistically significant (p < 0.01). Although homogeneous by light microscopy, apical granules proved to be of different electron densities by transmission electron microscopy.