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  • Küçük Resim
    Öğe
    Investigation of the Effects of Storage Period for Frozen Bull Semen on In Vitro Embryo Production
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Akyol, Numan; Ertem, Talha Burak; Varisli, Omer
    The aim of this study was to investigate some spermatological parameters of frozen Brown Swiss bull semen 32 years ago by flow cytometry and to determine how storage time in liquid nitrogen affects in vitro embryo production ratios. For this purpose, early necrotic, necrotic, viable and apoptotic spermatozoa concentrations were analyzed using the Flow Cytometry and then the in vitro fertilization abilities of these sperms were investigated. AnnexinV/PI-FITC (R) was used to determine the apoptotic changes with flow cytometric analysis. Oocytes were obtained from slaughtered cows in a local abattoir. Brown Swiss semen, frozen in the last two years, were used for the control group. Early necrotic spermatozoa levels in semen frozen 32 years ago were lower but necrotic spermatozoa levels were higher than in the control group, but the opposite result was encountered in the control group (P<0.01) according to flow cytometry findings. The cleavage ratio in vintage spermatozoa was found to be lower than in the control group (P<0.0z), the blastocyst ratio was also lower than in the control group (P<0.01). As a result, it was observed that some spermatological parameters can be changed negatively and it can be said that a long storage period may lower the fertility capabilities of Brown Swiss bull semen.
  • [ X ]
    Öğe
    The Effect of Epidural Application of FSH on Superovulatory Response in Eastern Anatolian Red Cow
    (2021) Satılmış, Muharrem; Yılmaz, Mehmet Ali; Sevgi, Ramazan; Karaşahin, Tahir; Kızıl, Sedat Hamdi; Okuroğlu, Alaeddin; Ertem, Talha Burak
    This study aimed to evaluate the superovulation response in Eastern Anatolian Red (EAR) cows treated with a single epidural injection or traditional administration of follicle stimulating hormone (FSH). Eight cows were randomly divided into two groups, as control (CG) and experiment (EG). Four replications were applied for each animal in the groups. A total of 16 studies were carried out in each group. In this study,300 mg intramuscular (i.m) FSH injection was applied with decreasing doses twice daily for four days to the cows in the CG. (70:70, 50:50, 20:20, 10:10 mg). On the other hand, 300 mg FSH was administered as 150 mg epidural and 150 mg intramuscular injection to the cows in EG. The number of untransferable embryos and the total CLs were found statistically significant (p<0.05). However, the number of transferable embryos and unfertilized oocyte (UFO)were found statistically insignificant among the groups (p>0.05). According to the obtained data, it was observed that the epidural administration of FSH for superovulation in EAR cows did not provide sufficient follicle and oocyte development. In conclusion, different studies with different superovulation protocols and FSH doses in local breed EAR cows will be needed for further studies.