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    Genetic Identification For Some Fusarium Spp. Using Random Amplified Polymorphic Dna Polymerase Chain Reaction (Rapd-Pcr) Technique
    (Parlar Scientific Publications (P S P), 2016) Keskin, Ayten Celebi; Acnik, Leyla; Araz, Aydan; Guler, Perihan
    The Fusarium species that is the cause of the disease that we used in our study takes an important place among microfungi that cause disease in agricultural plant species and economic loss. The subgroups and races of this species especially cause diseases with various symptoms on cereals such as wheat, barley, corn and clover, on fruit trees, and garden and ornamental plants. The definition of these microfungi that are pathogens at the genetic material level is important in terms of determining the disease they cause. The aim of the present study was to determine the genetic relationship among twenty isolates of Fusarium spp. (18 Fusarium species) isolated from wheat, maize and clover in Turkey using RAPD technique. In the present study, Random Amplified Polymorphic DNA (RAPD) markers were used to assess the genetic diversity within 20 isolates of Fusarium. RAPD analysis was performed with 16 decamer primers selected from a total of 30 primers. A total of 408 reproducible fragments were amplified by 16 RAPD primers. All the fragments were polymorphic (100%). The size of these fragments ranged from 100 to 3000 bp. The number of bands per primer varied between 14 and 39. Similaritiy indexes were calculated to determine the genetic relationships among these populations and subsequently dendograms based on Unweighted Pair-Group Method using Arithmetic Averages (UPGMA) was derived. Based on DNA finger prints, all isolates of Fusarium were categorized into three major clusters. The results demonstrated that RAPD analysis can be used for assessing the genetic diversity and the relationships among the Fusarium species.
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    Nutritional qualities and antioxidant activity of three edible oyster mushrooms (Pleurotus spp.)
    (Royal Netherlands Soc Agr Sci, 2015) Khatun, Selima; Islam, Aminul; Cakilcioglu, Ugur; Guler, Perihan; Chatterjee, Narayan Chandra
    Three species of oyster mushrooms (Pleurotus spp.) that are cultivated mostly throughout the year in the plains of India were studied for their nutritional value and their antioxidant properties. Highest protein content was found in Pleurotus florida (22-25% dw) followed by Pleurotus citrinopileatus (20-22% dw) and Pleurotus pulmonarius (15-18% dw). Cholesterol content was in the range of 0.6-0.8% dw, making them low-cholesterol, proteinaceous food. The antioxidant properties of the three species were of both enzymatical and non-enzymatical nature. Reducing power, chelating activity on Fe2+ and total phenol contents were higher in P. florida than in P. pulmonarius and P. citrinopileatus. With regard to antioxidative enzymes, P.florida had the highest peroxidase and superoxide dismutase activity whereas maximum catalase activity was found in P. pulmonarius. P. florida had higher antioxidative activity than P. pulmonarius and P. citrinopileatus thereby highlighting its nutraceutical values along with nutritional qualities. (C) 2012 Royal Netherlands Society for Agricultural Sciences. Published by Elsevier B.V. All rights reserved.
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    Screening the cultural characteristics of Hebeloma eburneum Malencon
    (Academic Journals, 2011) Guler, Perihan; Turkoglu, Aziz; Kunduz, Ilknur
    In this study, the cultural characteristics of Hebeloma eburneum Malencon (Basidiomycota, Bolbitiaceae) that included morphological and anatomical features and their natural spread in Turkey were investigated. Basidiomata of Hebeloma eburneum usually grows single or in groups, with fleshy caps of 4 to 8 cm in diameter. The cuticle of pileus is white and pinkish from the center toward the edges. Tissue fragments of H. eburneum basidiomata mycelium that formed white-pinkish pigmentation were investigated with light microscope and scanning electron microscopy (SEM). In SEM examinations, the surface of spores was not flat, but has a rough structure.
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    Somatic incompatibility in Agaricus bitorquis (Quel.) Sacc.
    (Academic Journals, 2008) Guler, Perihan
    The somatic incompatibility in Agaricus bitorquis was studied using ten wild strains. Heterokaryons from each isolate were all paired with the same unrelated heterokaryon and also paired together in all combinations. Two different types of somatic incompatible interaction were observed lightly or heavily pigmented lines developing between the two isolates. In addition to, some elements and photographs of hyphae were determined by means of scanning electron microscopy.