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    The effect of basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat model
    (Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2013) Ozpolat, Berkant; Gurpinar, Ozer Aylin; Ayva, Ebru Sebnem; Gazyagci, Serkal; Niyaz, Mehmet
    Background: This experimental study aims to investigate whether basic fibroblast growth factor, adipose tissue-derived from mesenchymal stem cells, or a combination of both, has an effect on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat model. Methods: During the first phase of the study, mesenchymal stem cells were isolated by the primary explant culture technique from the abdominal adipose tissue of rats. When the cells became confluent, they were passaged and characterized by using immunofluorescence staining technique. The cells were cryopreserved for an in vivo application. The rats were divided into four groups, including: the basic fibroblast growth factor (group 1), the mesenchymal stem cells (group 2), the mesenchymal stem cells and basic fibroblast growth factor (group 3) and control group (group 4). The rats were sacrified at day 60 and the anastomosis was evaluated macroscopically for granulation tissue formation, the stenosis and presence of tracheocutaneous fistula formation, and also microscopically for stenosis, epithelium regeneration, inflammation, collagen formation and neovascularization. Results: The inflammation was significantly lower in the study groups (p=0.004, p=0.014, p=0.004), whereas the collagen formation and epithelial regeneration were significantly higher in the study groups, compared to the control group (p=0.015, p=0.022, p=0.026 and p=0.002, p=0.001, p=0.002). Conclusion: Both basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells increased epithelial regeneration and connective tissue organization in this rat model. They may be used as an adjuvant therapy to surgical resection in patients undergoing tracheal resection.
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    Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique
    (Tubitak Scientific & Technical Research Council Turkey, 2012) Niyaz, Mehmet; Gurpinar, Ozer Aylin; Gunaydin, Serdar; Onur, Mehmet Ali
    In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class I, CD45, CD 106, and MHC Class II for characterization of mesenchymal stem cells. The results of flow cytometry analysis showed that these cells were mesenchymal stem cells. Half of the cells were cryopreserved at all passages for future applications. It is thought that these mesenchymal stem cells can be used in therapy of cardiovascular diseases as an alternative technique in the near future.