Yazar "Küçük, Ayşegül" seçeneğine göre listele
Listeleniyor 1 - 16 / 16
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effect of Fullerenol C60 on Erythrocyte Deformability During Ischaemia-Reperfusion Injury of Lower Extremity in Diabetic Rats(Gazi Univ, Fac Med, 2019) Kip, Gülay; Kartal, Hakan; Çomu, Faruk Metin; Polat, Yücel; Arslan, Mustafa; Küçük, AyşegülBackground: Fullerenol, a water-soluble C60-fullerene derivative synthesized by Chiang et al, has been demonstrated to be able to scavenge free radicals in vitro and in vivo. Although its protective effects have been already studied and shown in ischemia reperfusion (IR) injury, additional investigation is necessary for its effect on erythrocyte deformability. The purpose of our study was to look into the effects of fullerenol C60 on erythrocyte deformability in rat lower extremity ischemia reperfusion injury model. Materials and Methods: After approval of the Ethics Committee, 30 Wistar Albino rat were divided into 5 groups (n:6) as; Control (C), Diabetes (group D), diabetes+ fullerenol C60 group (DF), diabetes+ IR (group DIR) and diabetes IR+ fullerenol C60 (DIRF). 55 mg/kg streptozotocin was administered to the rats for diabetes. After the period of 72 hour, blood glucose concentration was mesured, 250 mg/dl and above were considered as diabetic rat. Four week after the formation of diabetes, rats were subjected to 2 hour ischemia and 2 hour reperfusion. Erythrocyte packs were prepared from heparinized blood samples and deformability measurements were performed. Results: The deformability index was significantly increased in diabetic rats; however, it was similar in group D, DF and DIRF. It was significantly increased in group DIR when compared to group C, D, DF and DIRF. The relative resistance was increased in I/R models. Conclusion: This study aimed to investigate the effects of IR on erythrocyte deformability which may lead to disturbance in blood flow and hence tissue perfusion in infrarenal rat aorta. We found that fullerenol C60 had beneficial effects by reversing undesirable effects of IR. In our opinion, further studies with larger volume are required to support our promising results.Öğe Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats(2018) Özer, Abdullah; Çomu, Faruk Metin; Küçük, Ayşegül; Kılıç, Yiğit; Mardin, Barış; Alkan, Metin; Oktar, Gürsel LeventAmaç: İskemi-reperfüzyon (İR) hasarı, doku iskemisi sonucu gerçekleşen bir olaylar zinciridir. Hücresel hasarı takiben reperfüzyon, aktif bir inflamatuar yanıta neden olur. Eritrosit deformabilitesi ve plazma viskozitesi doku ve organ perfüzyonunda kritik bir rol oynar. Bu çalışmada, ratlarda miyokardiyal İR hasarı sırasında levosimendan ve nigella sativa'nın eritrosit deformabilitesi üzerine etkisini araştırmayı amaçladık. Yöntem: Yirmi dört adet Wistar albino rat çalışmaya dahil edildi. Hayvanlar rastgele dört deney grubuna ayrıldı. Grup K'daki (kontrol grubu) ratların koroner arterlerine oklüzyon veya reperfüzyon yapılmadı. Miyokardiyal İR, sol ön inen koroner arterin 30 dakika süreyle bağlanması, ardından İR (İR), İR-levosimendan (24 µg/kg) (İRL) ve İR-nigella sativa (0.2 mL/kg) (İRNS) gruplarında 2 saat reperfüzyon ile gerçekleştirildi. Deformabiliteyi ölçmek için% 5 htc içeren bir PBS tampon çözeltisi ile oluşturulan eritrosit süspansiyonları kullanıldı. Bulgular: Sonuç olarak, kontrol grubuna kıyasla İR, eritrosit deformabilitesinin bir belirteci olan nispi direnci artırdı (p<0.05). Gruplar arasında ANOVA testi ile yapılan karşılaştırmalara göre anlamlı fark vardı (p<0.0001). Bonferroni testi ile yapılan düzeltmelerden sonra elde edilen sonuçlar şu şekildedir: İRL ve İRNS gruplarının karşılaştırmaları benzer sonuçlar vermiştir (p=0.764). İR grubunun değerleri kontrol, İRNS ve İRL gruplarından anlamlı derecede yüksekti (sırasıyla p<0.0001, p=0.001, p=0.003). Sonuç: İskemi reperfüzyon hasarı olan ratlarda eritrosit deformabilitesi azaldı. Bu yaralanma mikrodolaşımda daha fazla sorun yaratabilir. Levosimendan ve nigella sativa, bu tip yaralanmaların olumsuz etkilerini azaltmada yararlı olabilirÖğe Effects of esmolol on lung injury induced by lower extremity Ischemia-reperfusion(Bayrakol Medical Publisher, 2022) Sabuncu, Ülkü; Sezen, Şaban Cem; Küçük, Ayşegül; Salman, Nevriye; Sabuncu, Timucin; Kip, Gülay; Kurtipek, ÖmerAim: After hind limb ischemia-reperfusion (I/R), impairments in remote organs are frequent. Lung tissue is the organ most affected by the remote organ damage. The lung damage increases ventilatory support, the need for inotropic agents and mortality. Many drugs and methods have been used in attempts to prevent or reduce this damage. The aim of this study is to investigate the protective effects of esmolol infusion on lung tissue prior to I/R created in the lower extremity. Material and Methods: The study was performed between 11 and 14 April 2018 in Gazi University Experimental Animal Research Center, Ankara, Turkey. After obtaining ethics committee approval, 24 rats were randomly divided into 4 groups: Control (Group C), Esmolol (Group E), Ischemia-reperfusion (Group I/R), and I/R-Esmolol (Group I/RE). Esmolol (200 mu g/kg/min intravenous) was applied 30 minutes before the procedure. The biochemical and histopathological parameters of lung tissue samples were compared. Results: Neutrophil infiltration/aggregation, alveolar wall thickness, and total lung injury scores were significantly higher in the I/R group than in the C and E groups. In addition, neutrophil infiltration/aggregation, alveolar wall thickness, and total lung injury scores in the I/R group were statistically higher than in the I/R-E group (p=0.030, p=0.010, p=0.001, respectively). Malondialdehyde levels, catalase (CAT) and paraoxonase (PON) enzyme activities in the I/R group were significantly higher than in the C, E, and I/R-E groups. Glutathione S- transferase ( GSH) enzyme activity was similar in all groups. Discussion: It was found that esmolol infusion at 200 mu g/kg/min intravenously-reduced oxidative stress when administered 30 minutes before ischemia in rats and partially corrected the damage caused by I/R in lung histopathology.Öğe The Effects of HES 130/0.4 Application on Erythrocyte Deformability in Ureteral Obstructed Rats(2018) Güneş, Işın; Küçük, Ayşegül; Çomu, Faruk Metin; Şıvgın, Volkan; Alkan, Metin; Arslan, Mustafa; Ünal, YusufAmaç: Plazma genişleticilerinin uygulanması, önemli cerrahi, hemorajik şok ve travmayı içeren çeşitli klinik koşullardaki kritik hastaları yönetmek için çok önemlidir. Bununla birlikte, hidroksietil nişastanın (HES) eritrosit agregasyonu üzerindeki etkileri tartışmalıdır. Bu nedenle, deneysel tek taraflı üreteral tıkanıklık ile oluşturulan böbrek yetmezliğinde HES 130/0.4'ün eritrosit deformabilitesi üzerindeki etkilerini araştırmayı amaçladık. Yöntem: Toplam 24 rat kontrol, HES 130/0.4, Üreter Obstrüksiyon (ÜO) ve ÜOHES 130/0.4 olmak üzere dört gruba ayrıldı. Üreteral tıkanıklık gruplarına ketamin anestezisi altında düşük abdominal insizyon yapıldı. Sağ üreterin distaline ulaşıldıktan sonra 2.0 mersilen ile bağlandı, daha sonra geç dönemde böbrek yetmezliği için 3 hafta bekledi. 20 mL.kg-1 HES 130/0.4 (Voluven), HES 130/0.4 ve ÜO-HES 130/0.4 grubuna intravenöz olarak verildi. 24 saat sonra ratlar sakrifiye edildi. Deformabilite ölçümleri bir fosfat tamponlu salin (PBS) tamponu içinde% 5 hematokrit kullanılarak gerçekleştirildi. Bulgular: Her grupta rölatif rezistans kontrol grubuna göre arttı (p<0.0001). Her grupta eritrosit deformabilite indeksi kontrol grubuna göre anlamlı derecede yüksek bulundu (Grup K-Grup HES, p=0.023, Grup K-Grup ÜO, p<0.0001, Grup K-Grup ÜO-HES, p<0.0001) . ÜO-HES grubunda HES grubuna kıyasla eritrosit deformabilite indeksi önemli derecede arttı (p=0.031, p=0.021, sırasıyla). HES 130/0.4 uygulaması ile üreteral obstrüksiyon yapılmış sıçanlarda eritrosit deformabilitesinde herhangi bir değişiklik olmadığı belirlendi (p = 0.785). Sonuç: HES 130/0.4 kullanımı, üreteral obstrüksiyonu yapılan sıçanlarda eritrosit deformabilitesi üzerinde olumsuz bir etkiye sahip değildir. HES 130/0.4 kullanırken bu endikasyonların doğru kullanılması gerektiğini düşünüyoruzÖğe Effects of iron overload and exenatide on erythrocyte deformability in a rat model(Comenius Univ, 2015) Kuşkonmaz, Şerife Mehlika; Kara, Halil; Çomu, Faruk Metin; Küçük, Ayşegül; Arslan, MustafaIron overload is known to affect erythrocyte membrane properties and erythrocyte shape. We hypothesized that iron overload which directly affects the erythrocyte morphology may also interfere with erythrocyte deformability (ED). Exenatide, a glucagon like peptide -1 (GLP-1) analogue used in the treatment of diabetes, is known to have beneficial pleiotropic effects on endothelial function and blood flow which are different from its glucose-lowering effects. In our study we aimed to test the effect of iron overload on ED in a rat model (1) and to evaluate the effect of exenatide on ED in the same model (2). For this purpose, the animals were randomly divided into three groups, each containing 6 rats. Rats in the control group (Group C) were given intraperitoneal injections of saline as placebo. The second group (Group Fe) was given intraperitoneal iron dextran (60 mg/kg/day) five days a week for 4 weeks to develop iron overload. The third group (Group Fe + E) received subcutaneous injections of 10 mcg exenatide (Byetta (R) Lilly Pharma) in two divided doses for 4 weeks in addition to iron dextran. We observed that ED index was significantly higher in Group Fe when compared to Group C and Group Fe+E (p <0.0001, p <0.0001). Administration of exenatide to the iron-loaded rats (Group Fe +E) led to a significant decrease in the deformability index (p < 0.017) when compared to Group Fe. Our study shows a decrease in ED with iron and beneficial effect of exenatide on ED in the iron-overloaded rat model (Fig. 1, Ref. 24). Text in PDF www.elis.sk.Öğe Effects of iron overload and exenatide on erythrocyte deformability in a rat model (conferenceObject)(Wiley-Blackwell, 2015) Kuşkonmaz, Şerife Mehlika; Kara, Halil; Çomu, Faruk Metin; Küçük, Ayşegül; Arslan, Mustafa…Öğe Effects of Recurrent Sevoflurane Anesthesia on Erythrocyte Deformability in Experimentally Induced Alzheimer Rats(Gazi Univ, Fac Med, 2022) Küçük, Ayşegül; Çomu, Faruk Metin; Güney, Sevin; Erkent, Fatmanur Duruk; Işık, Berrin; Öztürk, Levent; Arslan, MustafaObjectives: The aim of this study was to investigate the association between Alzheimer's disease and erythrocyte deformability and to assess whether recurrent sevoflurane anesthesia influenced this association. Methods: A total of 24 Wistar albino rats were divided into four groups: Control, sevoflurane, Alzheimer, and Alzheimer + sevoflurane. The experimental Alzheimer model was prepared by intracerebroventricular injection of streptozotocin at a dose of 3 mg/kg (10 mu l) to the Alzheimer and Alzheimer + sevoflurane groups. Sevoflurane (2.3%) was administered to the sevoflurane and Alzheimer + sevoflurane groups 4 weeks after the surgery for 3 days and lasting for 2 hours per day. Blood samples were then collected for deformability measurements. Results: The deformability index was significantly increased in Alzheimer-induced rats, but the results for the Alzheimer and Alzheimer + sevoflurane groups were similar. The erythrocyte deformability index was significantly increased in the Alzheimer and Alzheimer + sevoflurane groups (p=0.004 p=0.001 respectively). However, there was no difference in deformability in the sevoflurane group (p= 0.496) Conclusion: Erythrocyte deformability was increased in the Alzheimer-induced rats; however recurrent sevoflurane anesthesia did not affect erythrocyte deformability.Öğe Investigation of Analgesic Minimum Effective Dose of Apelin-13 With Different Doses of Intraperitoneal Injections and its Effects on Kidney Tissue(WILEY, 2020) Dursun, Ali; Özdemir, Çagri; Sezen, Şaban; Küçük, Ayşegül; Arslan, Mustafa[Özet Yok]Öğe The effect of cerium oxide on erythrocyte deformability in ischemia-reperfusion injury in rats administered sevoflurane(Bayrakol Medical Publisher, 2022) Turicay, Aydin; Şıvgın, Volkan; Çomu, Faruk Metin; Küçük, Ayşegül; Özdemirkan, Aycan; Güneş, Işın; Arslan, MustafaAim: Ischemia-reperfusion (IR) injury is a common problem in vascular surgery. Acute IR damage observed in the lower extremities, especially in aortic surgery, occurs following temporary cross-clamping of the abdominal aorta. Disruption in blood rheology disrupts microvascular blood flow, leading to exacerbation of microangiopathy. It is known that drugs used for anesthesia affect blood rheology, which is affected by many factors. Therefore, we aimed to investigate the effects of cerium oxide on erythrocyte deformability before sevoflurane anesthesia in rats with lower extremity IR. Material and Methods: After approval by the ethics committee, 30 rats were randomly divided into 5 groups. Control (group C), IR (group IR), IR-cerium oxide (group IRCO), IR-sevoflurane (group IRS), IR-cerium oxide-sevoflurane (group IRCOS). Infrarenal abdominal aorta and atraumatic microvascular clamp were placed in IR groups 30 minutes after intraperitoneal cerium oxide was administered at a dose of 0.5 mg / kg. One hundred and twenty minutes later, the clamp was removed and reperfused for 120 minutes. Sevoflurane was applied at a rate of 2.3% at 4 L/min and 100% oxygen during IR for the minimum alveolar concentration to be 1 for rats. All rats were administered intraperitoneal ketamine (100 mg/kg) and euthanasia was performed by taking blood from the abdominal aorta. Erythrocytes were obtained from heparinized whole blood samples. Deformability measurements were made in erythrocyte suspensions in phosphate-buffered saline. A constant flow filtrometer system was used for the measurement of erythrocyte deformability and relative resistance was calculated. Results: Erythrocyte deformability index was found to be significantly different between the groups (p=0.002). Compared to the control group, the erythrocyte deformability index was significantly higher in IR and IRS groups (p<0.0001, p=0.003, respectively). In the IRCO and IRCOS groups, the erythrocyte deformability index was found to decrease significantly compared to the IR group (p=0.008, p=0.025, respectively). The erythrocyte deformability index was similar in Group C and in the IRCO and IRCOS groups (p=0.453, p=0.120, respectively). Discussion: We determined that cerium oxide administered intraperitoneally 30 minutes before ischemia in rats corrects the erythrocyte deformability deteriorated in IR-generated rats. We also found that cerium oxide had beneficial effects by reversing undesirable effects of IR. Further studies with larger volumes are required to support our promising resultsÖğe The effect of cerium oxide on lung injury following lower extremity ischemia-reperfusion injury in rats under desflurane anesthesia(Saudi Med J, 2021) Özdemirkan, Aycan; Küçük, Ayşegül; Güneş, Işın; Arslan, Mustafa; Tuncay, Aydin; Sivgin, Volkan; Sezen, Şaban C.Objectives: To examine the effects of desflurane and cerium oxide (CO) on lung tissue following ischemia-reperfusion injury (IRI). Methods: Experiments were conducted in Gazi University Animal Laboratory, Ankara, Turkey. Thirty rats were divided into 5 groups: control (C), IRI, IRI-CO, IRI-desflurane (IRID), IRI-CO-desflurane (IRICOD). Cerium oxide was given intraperitoneally. Lower extremity IRI was induced. Desflurane was applied during IRI. Lung histopathological examinations and serum biochemical analyses were performed. Results: Serum nitric oxide (NO) and malondialdehyde (MDA) levels were higher in group IRI (p=0.006) than in group C (p=0.001). Serum MDA and NO levels were significantly lower in groups IRICO and IRICOD than in group IRI. Significantly greater alveolar wall thickening and neutrophil infiltration were recorded in group IRI than in group C. Co-administration of desflurane and CO significantly decreased alveolar wall thickening and neutrophil infiltration compared to group IRI. Total lung injury scores were significantly lower in groups IRID, IRICO, and IRICOD than in group IRI. Conclusion: Intraperitoneal CO with desflurane, reduced oxidative stress and corrected the damage in lung. Cerium oxide given before and desflurane given during IRI have been shown to have protective effects on lung damage in rats.Öğe The Effect of Hydrogen-Rich Saline Solution on Erythrocyte Deformability in Lower Limb Ischemia Reperfusion Injury in Rats(Gazi Univ, Fac Med, 2021) Özer, Abdullah; Arslan, Mustafa; Çomu, Faruk Metin; Küçük, Ayşegül; Mardin, Baris; Koçak, Başak; Oktar, Gürsel LeventAim: We aimed to investigate the effects of HRSS on erythrocyte deformability in lower limb ischemia reperfusion (IR) injury in rats. Methods: Eighteen male Wistar albino rats used in this study. The animals were randomly divided into three experimental groups as control, IR and IR-HRSS. 20 mg.kg-1HRSS was administered (20 mg.kg(-1) i.p)30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. 120 min ischemia and 120 min reperfusion is applied to the groups. Erythrocytes were obtained from heparinized whole blood samples for deformability measurements. Kruskal-Wallis test was used for independent samples and Mann-Whitney U test was used to analyze differences between groups. Results: Ischemia reperfusion was found to increase relative resistance to the control group. The erythrocyte deformability index was significantly higher in IR and IR-HRSS groups than the control group. HRSS application significantly decreased erythrocyte deformability index compared to IR group. Conclusion: IR induced rats decreased erythrocyte deformability was partially corrected by HRSS. We believe that the protective effects of HRSS in IR injury and its use indications can be demonstrated in detail as long as the findings we have reached in our study are supported by other studies.Öğe The Effect of Repeated Doses of Intermittent Ketamine on Erythrocyte Deformability in Infant Rats(Gazi Univ, Fac Med, 2022) Kaşıkara, Hülya; Çomu, Faruk Metin; Küçük, Ayşegül; Öztürk, Levent; Arslan, MustafaAim: Blood rheology, which is affected by many factors, is also known to be affected by drugs used for anesthesia. Therefore, we aimed to investigate the effects of 21-day ketamine administration on erythrocyte deformability in 3-week-old infant rats Methods: Twelve 12 week old Wistar Albino male infant rats were randomly divided into two groups. Ketamine group (K), n:6, 50 mg/kg ketamine was administered intraperitoneally at the same time daily for 21 days. Saline group (S), n.6, 50 mg/kg normal saline was administered intraperitoneally at the same time daily for 21 days. After 21 days of administration, all rats were euthanized by intraperitoneal ketamine (100 mg/kg) and taken blood from abdominal aorta. Erythrocytes were obtained from heparinized blood samples. Deformability measurements were performed on erythrocyte suspensions in phosphate buffered saline. For the measurement of erythrocyte deformability, a constant flow filtrometer system was used and the relative resistance was calculated. Results: Ketamine administration was found to increase relative resistance. The erythrocyte deformability index was significantly higher in the ketamine group compared to the saline group (p:0.006). Conclusion: We found that long-term repeated ketamine administration negatively affected erythrocyte deformability. In our study, we think that the results obtained in daily anesthesia will be a guide for repeated administration of ketamine, especially in radiation oncology. However, these findings should be supported by clinical and experimental studies in more detailed and large series.Öğe The Effect of Sevoflurane and Fullerenol C 60 on the Liver and Kidney in Lower Extremity Ischemia-Reperfusion Injury in Mice with Streptozocin-Induced Diabetes(Dove Medical Press Ltd, 2023) Sengel, Necmiye; Küçük, Ayşegül; Özdemir, Cağrı; Sezen, Şaban Cem; Kip, Gülay; Er, Fatma; Dursun, Ali DoğanObjective: This study aimed to demonstrate whether fullerenol C60, sevoflurane anesthesia, or a combination of both had protective effects on the liver and kidneys in lower extremity ischemia-reperfusion injury (IRI) in mice with streptozocin-induced diabetes. Methods: A total of 46 Swiss albino mice were divided into six groups as follows: control group (group C, n=7), diabetes group (group D, n=7), diabetes-ischemia/reperfusion (group DIR, n=8), diabetes-ischemia/reperfusion-fullerenol C60 (group DIR-FC60, n=8), diabetes-ischemia/reperfusion-sevoflurane (group DIR-S, n=8), and the diabetes-ischemia/reperfusion-fullerenol C60-sevoflurane (group DIR-S-FC60, n=8). Fullerenol C60 (100mg/kg) was administered intraperitoneally 30 min before the ischemia-reperfusion procedure to the fullerenol groups (DIR-FC60 and DIR-S-FC60). In the DIR groups, 2 hours (h) ischemia-2h reperfusion periods were performed. In the sevoflurane groups, sevoflurane was applied during the ischemia-reperfusion period with 100% O2. Liver and kidney tissues were removed at the end of the reperfusion procedure for biochemical and histopathological examinations.Results: In liver tissue, hydropic degeneration, sinusoidal dilatation, pycnotic nuclei, prenecrotic cells, and mononuclear cell infiltration in parenchyma were significantly more frequent in group DIR than in groups D and group C. In terms of the histopathologic criteria examined, more positive results were seen in group DIR-FC60, and when group DIR-FC60 was compared with group DIR, the difference was significant. The best results in AST, ALT, glucose, TBARS levels, and SOD enzyme activities in liver tissue were in group DIR-FC60 compared with group DIR, followed by groups DIR-S-FC60 and DIR-S, respectively. Regarding TBARS levels and SOD enzyme activities in kidney tissue, the best results were in groups DIR-FC60, DIR-S-FC60, and DIR-S, respectively.Conclusion: According to our findings, it is clear that fullerenol C60 administered intraperitoneally 30 min before ischemia, alone or together with sevoflurane, reduces oxidative stress in distant organ damage caused by lower extremity IRI, and reduces liver and kidney tissue damage in histopathologic examinations.Öğe The Effects of Esmolol on Erythrocyte Deformability in Rat Liver Ischemia-Reperfusion Injury(Gazi Univ, Fac Med, 2021) Sabuncu, Ülkü; Küçük, Ayşegül; Çomu, Faruk Metin; Salman, Nevriye; Kip, Gülay; Ünal, Yusuf; Arslan, MustafaBackground: Esmolol has protective effects in ischemia reperfusion (IR) injury. The purpose of our study was to look into the effects of this which esmolol on erythrocyte deformability in rat liver IR injury model. Materials and Methods: We used 24 Wistar albino rats as subjects in our study. They were divided into 4 groups; randomized control group (group C; n=6), esmolol group 200 mu g/kg/min intravenously (group E; n=6), IR group (group IR; n=6) and IR group with esmolol 200 mu g/kg/min intravenously (group IR-E; n=6). Erythrocyte packs were prepared from heparinized blood samples and deformability measurements were performed. Results: It was discovered that ischemia reperfusion increased the relative resistance when compared to control group (p<0.0001). Erythrocyte deformibility index was found to be higher in IR and IR-E groups compared to control group (p<0.0001, p=0.002, respectively). Esmolol application decreased the erythrocyte deformibiltiy index when compared to control group (p=0.017). Conclusion: In this research, esmolol application has improved the ertyhrocyte deformibity in liver rat IR injury partially. We also found that esmolol had beneficial effects by reversing undesirable effects of IR. Further studies with larger volume are required to support our promising results.Öğe The effects of fullerenol nanoparticles on erythrocyte deformability in sevoflurane applied rats(Anaesthesia Pain & Intensive Care, 2021) Sivgin, Volkan; Küçük, Ayşegül; Çomu, Faruk Metin; Yalçın, Ayşe Gülfem; Arslan, MustafaBackground & objective: Oxidative damage causing alterations in erythrocyte deformability due to anesthesia might be one of the factors responsible for the deterioration of the tissue and organ perfusion. The antioxidant properties of fullerenol nanoparticles, used in medical field together with the developing technology, have been shown in the literature. We investigated the effects of fullerenol nanoparticles, used before sevoflurane anesthesia, on the erythrocyte deformability in the rats. Methodology: Twenty-four male Wistar Albino rats were used in this study and randomly divided into four groups, six in each group; Group C (Control Group), Group S (Group Sevoflurane), Group F (Group Fullerenol), Group FS (Group Fullerenol-Sevoflurane). Fullerenol was given to the Group F and Group FS at a dose of 100 mg/kg. Sevoflurane was administered to rats in the Groups S and FS at 3% concentration. Erytrocytes deformability was measured by the constant-current filtrometre system and the deformability index was interpreted. All the data were processed by variance analysis in SPSS 22.0 for Windows statistical software. Variance analysis and Kruskal-Wallis test were used to evaluate the data. Mann-Whitney U test with Bonferroni correction were used to evaluate the variables with significance. Results: Relative resistance increased in all groups due to sevoflurane administration (p < 0.0001). The erythrocyte deformability index was significantly higher in the sevoflurane group than in the control and fullerenol groups (p < 0.0001, p = 0.002, respectively). Fullerenol administration, before 30 min of sevoflurane treatment, was found to decrease erythrocyte deformability index significantly compared to sevoflurane group (p = 0.017). Conclusion: Administring 100 mg/kg fullerenol nanoparticles intraperitoneally 30 min before sevoflurane reduces erythrocyte deformability.Öğe The role of pomegranate seed oil on kidney and lung tissues in the treatment of sepsis: animal pre-clinical research(J Infection Developing Countries, 2023) Arslan, Mustafa; Küçük, Ayşegül; Bozok, Ümmü Gülsen; Ergoruen, Aydan Iremnur; Sezen, Şaban Cem; Yavuz, Aydin; Kavutcu, MustafaObjectives: Sepsis is a common disease with a high mortality. Decreasing the speed is possible with early and intensive therapy. However, most medicines have been tested, but none has proven effective. Therefore, the study aimed to discover the protective and therapeutic effects of pomegranate seed oil (PSO). Methods: The cecal ligation puncture (CLP) method was used to induce sepsis. The experimental procedure was started with the animals divided haphazardly into four groups: control (C), sepsis (CLP), CLP + low dose PSO (CLP + LD), and CLP + high dose PSO (CLP + HD). First, the cecum was filled with feces. The full cecum was tied under the ileocecal valve for ligation and punctured. At 1 hour after CLP, 0.32 mg/kg and 0.64 mg/kg of PSO were administered. 24 hours after, lung and kidney specimens were collected. Results: Neutrophil infiltration/aggregation and alveolar wall thickness decreased in lung with PSO groups compared with the CLP. The findings for overall lung injury were similar. In renal, all parameters were increased in the CLP compared with C, except for vascular vacuolization and hypertrophy. According to the CLP, all parameters were significantly lower in CLP + HD. Furthermore, glomerular vacuolization, degeneration, and necrosis of tubular cell, dilatation of bowman space, and tubular hyaline cylinders reduced CLP + LD versus CLP. Thiobarbituric acid-reactive substances decreased in lung, with the PSO groups. In addition, superoxide dismutase increased in PSO groups versus CLP. Conclusions: We conclude that the high-dose PSO is especially effective in treating sepsis.
