Yazar "Kabalci, Mehmet" seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe The antibacterial effect of mesenchymal stem cells on graft infections: An experimental study(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2018) Kabalci, Mehmet; Canbeyli, Ibrahim Deniz; Eroglu, ErdincBackground: In this study, we aimed to investigate the antibacterial effects of mesenchymal stem cells, compared to tigecycline, on graft infection related with methicillin-resistant Staphylococcus epidermidis in a rat model. Methods: A total of 42 male adult Wistar rats (age >6 months; weight 300 to 350 g) were divided into six groups including seven rats in each. Group 0 did not undergo any procedure; Group 1 was infected, but untreated; Group 2 was infected and treated with tigecycline without graft placement; Group 3 was infected and received mesenchymal stem cells without graft placement; Group 4 was infected and treated with tigecycline after graft placement; Group 5 was infected and treated with mesenchymal stem cells after graft placement. The pockets created were either left empty or implanted with Dacron grafts. Treatment was commenced at 48 h. Specimens were collected on Day 13. Perigraft tissues were evaluated histopathologically and bacterial colony numbers were counted. Results: No bacterial colonization was observed in Group 0, whereas there was a significant colonization in Group 1. Complete eradication was achieved in Group 2 and Group 3 (graft-free groups), and near-complete eradication was achieved in Group 4 and Group 5 (graft-implanted groups). The histopathological findings significantly differed between Group 1-Group 2 and between Group 1-Group 3 (graft-free groups). The histopathological findings were similar between Group 2-Group 3 and between Group 4-Group 5. Conclusion: Our study results suggest that mesenchymal stem cells may be a novel, contemporary alternative to antibiotherapy and may decrease the bio-burden of Staphylococcus at the infected graft areas, and mesenchymal stem cell treatment may be as effective as tigecycline.Öğe Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions(WALTER DE GRUYTER GMBH, 2020) Kabalci, Mehmet; Sahin, Mustafa; Pekcan, Zeynep; Zengin, Mehmet; Dogru, Mehmet Tolga; Kisa, UclerBackground: To investigate and compare the antiadhesive/antifibrotic effects of mesenchymal stem cells (MSC) and amnion membrane transfer (AMT) in a rat model. Material and methods: Three experimental and sham groups were formed using 30 Wistar-Albino rats. AMT and MSC were applied to the related groups. The control group was not treated. After 12 weeks follow-up, intracardiac blood and cardiac-pericardiac tissue samples were taken. The severity of adhesions and fibrosis were scored macroscopically and microscopically with Hematoxylin/ Eosin and Masson's trichrome staining. TNF-alpha, TGF-beta, IL-1, PDGF, FGF, VEGF and Caspase-3 levels were measured with the ELISA method. Results: Severe adhesions were observed in the AMT and control groups, but no adhesion was present in the MSC group. Pericardial thickness, increased vascularity, fibrosis, and collagen accumulation were similar between control and AMT groups, but were less in Sham and MSC groups. Between MSC and AMT groups, only Caspase-3 level was different, which is an apoptosis marker. Conclusion: The positive effects of MSC on adhesion, which we achieved in our study, suggest that it may prevent adhesion. AMT did not provide a positive effect. The correlation of Caspase-3 with postoperative adhesion/fibrosis should be examined in more detail.Öğe Electrodeposition of Zn/TiO2 coatings on Ti6Al4V produced by selective laser melting, the characterization and corrosion resistance(Iop Publishing Ltd, 2024) Gunduz, Demet Ozaydin; Kucukturk, Gokhan; Pul, Muharrem; Salunkhe, Sachin; Kaya, Duran; Kabalci, Mehmet; Cep, RobertRecently, additive manufacturing techniques have begun to be implemented extensively in the production of implants. Ti6Al4V alloy is a material of choice for implants due to its low density and high biocompatibility. Recent research, however, has demonstrated that Ti6Al4V alloy emits long-term ions (such as Al and V) that are hazardous to health. Surface modifications, including coating, are therefore required for implants. The electrodeposition method was utilized to deposit Zn-doped TiO2 onto the surfaces of Ti6Al4V samples, which were manufactured via the selective laser melting method. The effects of processing time, amount of TiO2 addition, microstructure of anode materials, and resistance to wear and corrosion were investigated. The coating hardness and thickness increased with increasing processing time and TiO2 concentration. It has been observed that the addition of TiO2 to zinc anode coatings results in an increase in wear and a decrease in corrosion rate. It was noted that the specimens exhibiting the most significant wear also possessed the highest hardness value. The specimens were generated utilizing a graphite anode, underwent a 30-min processing time, and comprised 10 g l(-1) of TiO2.Öğe Evaluation of physical activity level and exercise capacity in patients with varicose veins and chronic venous insufficiency(Sage Publications Inc, 2021) Erdal, Elif Sakizli; Demirguc, Arzu; Kabalci, Mehmet; Demirtas, HuseyinObjective The clinical indication of chronic venous insufficiency (CVI) is related to functional performance and the benefits of physical activity in patients with CVI are known. Despite its importance, the literature is limited in this regard. This study aimed to determine exercise capacity and physical activity level in patients with varicose veins and CVI. Methods Volunteers who came to the polyclinic with leg pain complaints were enrolled in the study. Individual sociodemographic and clinical information was recorded. Individuals' pain severity was assessed by the visual analog scale (VAS) and exercise capacity was assessed by 6-minute walk test (6MWT). International Physical Activity Questionnaire (IPAQ) was used to assess the level of physical activity. Results The study group consisted of 51 individuals who were diagnosed with varicose veins and CVI. The control group consisted of 51 individuals without varicose veins and CVI diagnosis. In the study group, the VAS during activity was significantly higher compared to the control group. The 6MWT distance, distance %, IPAQ total score and IPAQ walking score of the control group were significantly higher in comparison with the study group (p<0.05). Conclusions We believe that our findings will lead the planning of interventions to increase the level of physical activity in CVI patients.Öğe Mesenchymal stem cells have significant anti-infective effect on methicillin-resistant Staphylococcus epidermidis vascular graft infections(Turkish Joint Diseases Foundation, 2019) Canbeyli, Ibrahim Deniz; Kabalci, Mehmet; Cirpar, Meric; Tiryaki, Meral; Oktas, BirhanObjectives: This study aims to evaluate the effects of mesenchymal stem cell (MSC) implantation on vascular graft infections caused by methicillin-resistant Staphylococcus epidermidis (MRSE) and compare with antibiotic treatment. Materials and methods: Healthy adult 56 Wistar rats (age, over 5 months: weighing. 300-350 g) were divided into eight groups. Group I was defined as the control group and group 2 was defined as the infected control group. Groups 3 and 4 were defined as Dacron grafted and MRSE infected groups. treated with tigecycline and MSCs. respectively. Groups 5 and 6 were performed polytetrafluoroethylene (PTFE) graft and infected with MRSE. These groups were also administered tigecycline and MSC treatment. respectively. Groups 7 and 8 were infected with MRSE without graft administration and were also performed tigecycline and MSC treatment. respectively. Grafts and soft tissue specimens were collected at 13 days postoperatively. Colony counts of pert-graft tissue were performed. All samples were evaluated by enzyme-linked immunosorbent assay (ELISA) for the markers that determine stem cell activity. Results: The overall success of the treatments was assessed by the number of rats with MRSE recurrence, regardless of graft used. The difference between the untreated group 2, tigecycline groups (3. 5 and 7) and MSCs groups (4.6 and 8) were statistically significant. Success of MSC and tigecycline treatments was similar in Dacron. PTFE, and non-grafted groups. There was a resistance of MRSE infection in Dacron groups to MSC and tigecycline treatments. This was considered to be indicative of the susceptibility of the Dacron grafts to infection. However, there was no significant difference between group 2 and Dacron groups in terms of bacterial colonization. ELISA results were significant in three cytokines. Conclusion: Mesenchymal stem cells can be considered as an alternative treatment option on its own or part of a combination therapy for control of vascular graft infections.Öğe Mid-term results in the treatment of varicose veins with N-butyl cyanoacrylate(Sage Publications Inc, 2017) Eroglu, Erdinc; Yasim, Alptekin; Ari, Murat; Ekerbicer, Hasan; Kocarslan, Aydemir; Kabalci, Mehmet; Acipayam, MehmetAim To present mid-term results of patients with varicose veins treated with N-butyl cyanoacrylate (VariClose (R)), a nontumescent endovenous ablation technique. Patients and method Endovenous ablation was performed on 180 patients with saphenous vein incompetence between May and October 2014. One hundred sixty-eight subjects capable of being followed-up for 30 months were included. Patients' pre- and postoperative data were recorded. Results Procedures were performed on the great saphenous vein in 159 patients and on the small saphenous vein in nine patients. Saphenous vein diameters ranged between 5.5mm and 14mm. Full ablation was achieved in all patients following the procedure. No complications were encountered. Patients were monitored for 30 months. Ablation rates were 100% at the 3(rd) month, 98.3% at the 6(th) month, 96.6% at 1 year, and 94.1% at 30 months. Mean venous clinical severity score was 10.2 before procedures, decreasing to 3.9 at 3 months, 4.2 at 6 months, 2.9 at 12 months, and 2.7 at 30 months (p=0.000). Conclusion Due to its high success rate, absence of complications, no tumescent anesthesia requirement and high patient satisfaction, endovenous ablation with N-butyl cyanoacrylate is a good method. However, long-term follow-up results are now needed.
