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  • Küçük Resim
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    The Change in Luteal Blood Flow and Luteal Size after Beta Carotene and GnRH Injections in Early Pregnant Dairy Cows
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Ay, Serhan Serhat; Kucukaslan, Ibrahim; Kaya, Duygu; Mulazimoglu, Serkan Baris; Emre, Birten; Kacar, Cihan; Aslan, Selim
    The aim of this study was to determine the effectiveness of intramuscular injections of beta-carotene (beta C) and GnRH on luteal size (LS), luteal blood flow (LBF) and serum beta C concentrations in early pregnant cows. Twenty-nine Holstein-Friesian cows with a mature corpus luteum (>19mm) were randomly assigned to two groups: pc not received (beta C-; n=15) or received (beta C-; n=14). All cows were treated with PGF(2)alpha and inseminated twice, 48 and 72h after the treatment. Last Al was considered to be day 0. All cows received GnRH on day inseminations, 7 and 17. Different from the beta C-, the beta C+ group received beta C intramuscularly on day 7 and 17. In both groups, measurement of LS and LBF were performed on days 7, 10, 17,27 and 37 by transrectal B-mode and colour Doppler ultrasonography. Blood samples were collected on each examination day. Only cows that became pregnant were included in the statistical evaluation. The concentration o beta C in the beta C+ group was higher than in the beta C- at all examination days except day 17 (P<0.05). There was no significant difference between groups concerning the progesterone concentrations (P>0.05). The LS and LBF of beta C+ group on day 7 (P<0.05) and 27 (P<0.01) was higher than in the beta C- group and values increased significantly until day 37 (LS: P<0.05, LBF: P<0.01). We conclude that beta C injections significantly increased serum beta C concentrations, as well as LS and LBF.
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    Öğe
    Effects of injectable trace element and vitamin supplementation during the gestational, peri-parturient, or early lactational periods on neutrophil functions and pregnancy rate in dairy cows
    (Elsevier, 2021) Yazlik, Murat Onur; Colakoglu, Hatice Esra; Pekcan, Mert; Kaya, Ufuk; Kuplulu, Sukru; Kacar, Cihan; Polat, Mert
    The aim of this study was to evaluate effects of injectable trace element and vitamin combination on phagocytic, oxidative burst activity of neutrophils and reproductive outcomes in dairy cows. Cows were to assigned to the following groups: (1) injectable trace element supplementation (ITES, n = 44, containing zinc, manganese, copper, selenium); (2) injectable vitamin supplementation (VIT, n = 48, containing vitamins A, D-3, E); (3) ITES + VIT = 46); and (4) control (CON, n = 44). Cows were administered four injections between 230 and 260 days of the gestational period, on day of parturition, and 30 days postpartum. Neutrophil function was assessed at 10 days before and after calving. Phagocytosis was greater in cows of the ITES + VIT group at 10 days prepartum (P < 0.05) while oxidative burst was similar among groups. There were greater non-esterified fatty acid (NEFA) concentrations in cows of the ITES+VIT group at 10 days prepartum (P < 0.05). Cows supplemented with ITES+ VIT had less SOD activity than those supplemented with ITES or vitamin during the pre- to post-partum transition period (P < 0.05). The total odds of pregnancy were greater in cows supplemented with trace element and/or vitamin (P < 0.05). In conclusion, supplementation of ITES and/or VIT resulted in an increased total pregnancy rate. Vitamin or trace element supplementation did not differ with the control group in both the prepartum and postpartum period for immune variables. There, however, was greater phagocytosis in cows supplemented with vitamin and trace elements during the prepartum period that might be related to metabolic-induced inflammation.