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    Comparison of the Mechanical Properties of Platelet-Rich Fibrin and Ankaferd Blood Stopper-loaded Platelet-rich Fibrin
    (Wolters Kluwer Medknow Publications, 2018) Aktas, A.; Ozer, T.; Sen, M.; Turk, M.; Karabulut, E.
    Background and Aim: Platelet-rich fibrin (PRF) can be named as a natural fibrin-based biomaterial favorable to increasing vascularization and able to guide epithelial cell migration to its surface. The membrane has a significant positive effect on protecting open wounds and accelerating healing. Similar to PRF Ankaferd Blood Stopper (ABS) also has positive effects on wound healing. The aim of this study was to detect if we can improve known physical properties of PRF combining with ABS. This idea was based on the known mechanism of ABS in forming protein network without damaging any blood cells. Materials and Methods: A total of 25 adult rabbits used for collecting 5-7 ml of blood passively with the help of winged blood collection needle to the test tube. Collected samples were centrifuged at 3000 rpm for 10 min. Two similar samples obtained from each animal and one of the samples was placed in 20% ABS 80% saline solution for 5 min. Mechanical properties of the membrane samples were measured using Universal Testing Machine. Results: There is the statistically significant difference between PRF and ABS added PRF in elongation/mm (dL) and elongation/% at break values. Maximum force (fMax) and modulus values did not show any statistically significant differences. Conclusion: ABS loaded PRF causes better physical properties. This combination seems to exhibit superior performance when used as a membrane barrier solely. Advanced studies can be done on biological properties of ABS loaded PRF, especially on tissue healing.
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    The efficacy of platelet-rich plasma gel in MRSA-related surgical wound infection treatment: an experimental study in an animal model
    (Springer Heidelberg, 2018) Cetinkaya, R. A.; Yilmaz, S.; Unlu, A.; Petrone, P.; Marini, C.; Karabulut, E.; Urkan, M.
    IntroductionThe wound healing properties of platelet-rich plasma (PRP) gel have been documented in many studies. PRP gel has also become a promising agent for treating surgical site infections. In this study, we investigated the antibacterial activity and wound healing effectiveness of PRP in an animal model of Methicillin-resistant Staphylococcus aureus subsp. aureus (MRSA N315)-contaminated superficial soft tissue wounds.Materials and methodsSubcutaneous wounds in Wistar Albino male rats were created by making two cm midline incisions followed by inoculation of microorganisms. Study groups comprised of Sham (no treatment), PRP alone, MRSA alone, MRSA+PRP, MRSA+Vancomycin, and MRSA+Vancomycin+PRP groups. We inoculated 0.1mL (3x10(8) CFU/mL) of MRSA in contaminated groups. After 8 days, all rats were killed, wounds were excised and subjected to histopathologic examination, and MRSA counts were determined.ResultsMRSA counts in MRSA, MRSA+PRP, MRSA+Vancomycin and MRSA+Vancomycin+PRP groups were 5.1x10(6) (SD0.4) CFU/mL, 4.3x10(6) (SD +/- 0.7) CFU/mL, 2.3x10(6) (SD +/- 0.3) CFU/mL, 1.1x10(6) (SD +/- 0.4) CFU/mL, respectively. The inflammation scores of MRSA+PRP, MRSA+Vancomycin, and MRSA+Vancomycin+PRP groups were significantly lower than the MRSA group. MRSA+Vancomycin+PRP group inflammation score was significantly lower than the MRSA+PRP group.DiscussionAll treatment groups were effective in wound healing and decreasing the MRSA counts. MRSA+PRP combined created identical inflammation scores to the PRP group. More in vivo studies are required to corroborate these findings.