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Öğe The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality(Scientific Technical Research Council Turkey-Tubitak, 2015) Karasahin, Tahir; Arikan, SevketThe present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 mu M). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 mu M) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morulablastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.Öğe The Effects of Low Doses of FSH and Season on the in Vitro Maturation of Bovine Oocytes(Wiley-Blackwell, 2015) Karasahin, Tahir; Arikan, Sevket…Öğe Investigation of Bull Effect on in vitro Embryo Production(Kafkas Univ, Veteriner Fakultesi Dergisi, 2014) Akyol, Numan; Kizil, Sedat Hamdi; Satilmis, Muharrem; Karasahin, TahirThe aim of the study was to show whether there were some differences among 9 Holstein bulls and within their replications on their ability of in vitro fertilization for in vitro embryo production as cleavage and coming into the blastocyst stage. Semen collected and frozen from nine Holstein bulls with satisfactory in vivo fertilization capabilities for artificial insemination was used for in vitro fertilization. Direct washing method by Brackett and Oliphant medium and 5 or 6 h incubation period were used for in vitro fertilization. Charles Rosenkrans medium was used for in vitro embryo culture. An atmosphere with a higher than 95% relative humidity, 39 degrees C, 5% CO2 and was used for all in vitro embryo production processes. Totally 2519 A and B quality oocytes were treated for in vitro embryo production. As a result statistically significant (P<0.05) variation was found for cleavage and blastocyst development among bulls. However there was no significant difference (P>0.05) between the replications for each bull as cleavage and coming into blastocyst stage. The results showed varied capabilities of bulls for in vitro fertilization and embryo production and male factor can affect success of in vitro embryo production.Öğe Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos(Scientific Technical Research Council Turkey-Tubitak, 2014) Karasahin, Tahir; Akyol, Numan; Satilmis, Muharrem; Kizil, Sedat Hamdi; Bucak, Mustafa Numan; Coyan, KenanThe objective of this study was to investigate the conception rates achieved with the transfer of in-vivo derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%.Öğe The results of consecutive superovulations in cows by induction with various exogenous progesterone routes(Scientific Technical Research Council Turkey-Tubitak, 2014) Akyol, Numan; Kizil, Sedat Hamdi; Satilmis, Muharrem; Karasahin, Tahir; Erat, SerkanThe aim of this study was to determine the possibility of yielding more transferable embryos from cows superovulated with/without exogenous progesterone (ear implant and intravaginal device). Two experiments were conducted in Holstein Friesian cows to evaluate the effect of exogenous progesterone on the yield of corpus luteum (CL), total embryo and ova, and transferable embryos before superovulation. A superovulation program using 2 different progesterone resources was applied (G1 = intravaginal device, G2 = ear implant). Superovulation and uterus irrigations were done at 28-day intervals in the treatment groups. Cows in the control group were superovulated at 60-day intervals without application of exogenous progesterone. The mean numbers of CLs and transferable embryos for the G1, G2, and control groups were, respectively 4.82 +/- 0.29, 6.71 +/- 0.29, and 7.81 +/- 0.31 for CLs and 0.86 +/- 0.35, 3.50 +/- 0.35, and 1.53 +/- 0.39 for transferable embryos. It was shown that exogenous manipulation of the estrus cycle by progesterone could be applied without superovulation intervals in Holstein cows. It may also be postulated that the ear implant was more effective than the intravaginal device as a progesterone source in the superovulation program.Öğe Vitrification of Bovine Embryos Developed in Vitro in Culture Medium Containing Oleic and Linoleic Acids(Wiley-Blackwell, 2015) Karasahin, Tahir; Arikan, Sevket…
