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Öğe Comparison of microbial protein synthesis and nutrient digestibility of Medicago sativa and Prangos pabularia hay in sheep(Agricultural Research Communication Centre, 2018) Guney, Mehtap; Erdogan, Sibel; Karsli, Mehmet Akif; Demirel, MuratThe aim of this study was to compare feed intake, nutrients digestibility, protein fractions entering into the duodenum, and ruminal fermentation parameters of sheep fed Medicago sativa (alfalfa) and Prangos pabularia, locally called kerkol hays. In the study three ruminally and duodenally cannulated Morkaraman sheep were used. It was carried out as two periods within a 2x3 crossover experimental design. Daily intake of crude protein (CP) was higher (P< 0.05) in sheep fed alfalfa than those fed kerkol hay. Digestibility of NDF and ADF were lower (P< 0.05) in sheep fed alfalfa hay than those fed kerkol hay (P< 0.05). The percentage of protein escaping ruminal degradation was higher (P< 0.05) in sheep fed kerkol hay (30.73%) than those fed alfalfa hay (15.05%). Ruminal fermentation parameters were similar between groups, except ruminal NH3-N concentration. It is concluded that kerkol hay can be used as forage for sheep feeding.Öğe Determinating the relationship between starch level and acidosis in high starch containing diets in lambs(Sivar-Soc Italiana Veterinari Animali Reddito, 2024) Evci, Sevket; Karsli, Mehmet AkifThe aim of this study was to determine the effects of high starch levels in the diet on the formation of subacute acidosis in lambs and to determine the ideal starch level in lamb fattening diet. Three different diets with starch content of 25% (1st Ration-R1), 30% (2nd Ration-R2) and 32% (3rd Ration-R3) were prepared to induce subacute rumen acidosis to achieve this purpose. These diets were fed to 6 Akkaraman lambs, aged 11-12 months, with a body weight of 30-35 kg, in a replicated 3x3 Latin Square design to determine daily nutrient consumptions and digestibility of nutrients. Rumen fermentation parameters (pH, organic acid and ammonia nitrogen) were determined in the rumen fluid sampled at 0, 2, 4, 6, 8 hours post-feeding in each period. Blood samples were also taken at 0- and 6-hours post-feeding in order to determine blood gases and blood biochemistry parameters. Blood glucose, total protein, triglyceride, urea, creatinine and albumin, pH, pCO(2), HCO3 and base clearance were measured in these blood samples. It was observed that the daily nutrient consumptions of animals decreased in parallel with the increases in starch in the diets (P<0.05). Similarly, in parallel with the increase in the starch level of the diet, there was a general tendency to decrease in digestion of nutrients other than starch (P=0.08). There was no statistical difference in general between rumen pH, total organic acid, organic acid ratios, only rumen ammonia levels were found to differ between the groups before and immediately after feeding (2nd hour) (P<0.05). While blood pH values and HCO3 values decreased after feeding, no significant change was observed in other parameters. Among the blood biochemistry parameters, only creatinine decreased statistically (P<0.01) in the R1 group after feeding, and no difference was observed between other parameters. There was no significant difference among the groups in any of the blood parameters. It can be concluded that increasing the starch level above 25% in the diets of lambs adversely affects the nutrient consumption and nutrient digestion of the animals in general, and also reduces the blood pH and HCO3 levels, thus creating a risk of subacute acidosis.Öğe Determination of in situ degradation kinetics of some legume waste not used for human consumption(Scientific Technical Research Council Turkey-Tubitak, 2018) Evci, Sevket; Karsli, Mehmet AkifThe objective of this experiment was to evaluate the chemical composition, in situ ruminal degradation kinetics, and protein fractions of subsieve dry beans (DB), chickpeas (CCP), red lentils (RL), and green lentils (CL). Four samples of those legumes were utilized as replicates and were incubated for up to 48 h in the rumen of 3 rams. RL and CCP had higher organic matter (OM) than DB and GL. Crude protein (CP) was the highest in GL (P < 0.05). Ether extract (EE) concentrations were higher in CCP and 1)11 compared to those of RL and CL (P < 0.05). Crude fiber (CF), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents were the highest in RL (P < 0.05). The acid insoluble nitrogen (ADIN-N) content was similar in all samples evaluated. While RI, had the lowest OM and CP degradability and water-soluble OM and CP concentrations, DB and CCP had the highest potentially degradable OM and CP contents at the end of the 48 h incubation period (P < 0.05). Escaped protein content was the highest in RL and the lowest in DB (P < 0.05). In conclusion, some of the pulse species that are produced locally and not suitable for human consumption can be used in the diet of ruminant animals as a source of protein based on OM and CP degradabilities and escape protein contents.Öğe Determination of nutrient content and digestibility characteristics of Prangos ferulacea (L.) on grazing lands of Eastern Anatolia(Scientific Technical Research Council Turkey-Tubitak, 2015) Aldemir, Resit; Bingol, Nuriye Tugba; Karsli, Mehmet Akif; Akca, IsmailThe aim of this study was to compare nutrient content, and in situ protein and organic matter degradation kinetics of Prangos ferulacea (L.) (locally called helis), a naturally growing plant on the top of Mount Artos, with those of alfalfa. P. ferulacea plants used in the study were collected from three different parts of Mount Artos in Van Province over two years. While organic matter content and in vitro organic matter digestibility of P. ferulacea were significantly higher, neutral detergent fiber, acid detergent fiber, and acid detergent insoluble nitrogen contents were significantly lower compared with those of alfalfa (P < 0.01). In general, in situ dry matter, and organic matter and crude protein degradability of P. ferulacea were significantly higher compared with those of alfalfa starting from 6 h of incubation (P < 0.05). Organic matter and crude protein degradability of P. ferulacea was 79.15% and 85.31%, respectively, after 48 h of incubation. By-pass protein content and both organic matter and crude protein degradation rates were similar between samples (P > 0.05). It can be concluded that P. ferulacea has a better nutrient quality compared with even high quality alfalfa based on nutrient contents and digestibility values, and therefore substitution of alfalfa by P. ferulacea in the diet of ruminant animals is feasible.Öğe Effect of genotype and non-genetic factors on growth traits and survival rates in Turkish indigenous Hair goats and their first cross with Boer bucks(Agricultural Research Communication Centre, 2017) Bolacali, Memis; Ozturk, Yahya; Yilmaz, Orhan; Kucuk, Mursel; Karsli, Mehmet AkifThe aim of this study was to determine the effects of genotype and non-genetic factors on the growth traits and survival rates of Turkish indigenous Hair goats (n=63) and their first crosses with Boer bucks (n=91), reared under a semi-intensive management system in the Van province of Turkey. The live weights of the kids at birth and on days 30, 60, 90, 120, 150, and 180 were determined to be 3.64, 8.89, 13.99, 18.97, 23.62, 27.22 and 30.44 kg, respectively. The average daily gains in the pre-weaning, post-weaning and overall periods were 171.09, 136.43 and 148.34 g, respectively. The multiple-birth kids had lower survival rates than the single-birth kids (P<0.01). As a result, it may be recommended to Hair goat breeders in the region to use Boer bucks in cross-breeding in order to ensure a better growth performance from the kids; to apply a sustainable selection program; and to improve management conditions. In addition, the data obtained from this study may be used as a model for designing policies on Hair goat breeding in this region.Öğe Effects of High-Fructose Corn Syrup Addition to Broiler Diets on Performance, Carcass Yield, Visceral Weights, Gut pH and Some Blood Parameters(Wiley, 2024) Sen, Gokhan; Demirci, Mehmet; Evci, Sevket; Senol, Ali; Karsli, Mehmet AkifBackground: This study hypothesizes that using different amounts of high-fructose corn syrup (HFCS) in broiler diets may improve performance. ObjectivesThis study aimed to determine the effects of HFCS added to broiler diets on performance, cecum pH and some biochemical parameters. Methods: A total of 120 Ross 308 chicks at the age of 0 day were divided into three main groups with four subgroups each. The groups consisted of a control (CON), low-HFCS and high-HFCS groups. The CON group received a diet containing no HFCS, the low-HFCS diet contained 50 mg/kg HFCS, and the high-HFCS diet contained 100 mg/kg HFCS. Body weight gain, feed consumption, carcass weight, visceral weight and cecum pH values were examined as performance parameters. Blood samples were taken at the end of the experiment and used to spectrophotometrically determine triglyceride, total cholesterol, high-density lipoprotein (HDL-CHO), low-density lipoprotein (LDL-CHO), glucose (GLU), creatinine (CRE), uric acid and insulin concentrations, as well as aspartate aminotransferase and alanine aminotransferase activities and oxidative stress markers. Proinflammatory cytokine levels were measured using ELISA test kits. Results: Feed consumption and body weight gain of the high-HFCS group decreased (p < 0.01). The feed conversion rate was negatively affected in both HFCS groups compared to the CON group (p < 0.01). The carcass yields of the groups linearly decreased with the increase of HFCS (p < 0.001). Serum LDL cholesterol (p < 0.05) and GLU (p < 0.01) levels were significantly lower in the HFCS groups than the CON. Serum CRE levels were higher in the low-HFCS group compared to the other groups (p < 0.001). The oxidative stress index (OSI) levels were lower in the low-HFCS group than the CON group (p < 0.05). Conclusion: The addition of 100 mg/kg HFCS to broiler diets negatively affected performance parameters, but HFCS supplementation positively affected biochemical parameters. In particular, low-HFCS supplementation decreased the OSI, indicating that it could possibly reduce oxidative stress. Accordingly, HFCS could be added to broiler diets at a level of 50 mg/kg.Öğe Effects of Supplemental Epigallocatechin Gallate in the Diet of Broilers Exposed to Fluoride Intoxication(Humana Press Inc, 2018) Aydogan, Ilkay; Karsli, Mehmet Akif; Basalan, Mehmet; Yildirim, Ebru; Cinar, Miyase; Sen, Goekhan; Sumer, TugceWe evaluated the effects of dietary epigallocatechin gallate (EGCG) on the performance, biochemical parameters, and liver histopathology of fluoride-intoxicated broiler chickens. In total, 160 1-day-old male broiler chicks (Ross PM3 strain) were collected and assigned to four groups (40 animals each), with four replicates. The control group received a basal diet; the F group received 800mg/kg fluoride; the EGCG group received 400mg/kg EGCG; and the EGCG+F group received 400mg/kg EGCG and 800mg/kg fluoride. The live weight (LW) of F-treated chicks was significantly lower than that of the controls. In the F-treated groups, feed intake (FI) and LW values were lower, but the feed conversion ratio (FCR) was higher than those of the controls. The ratio of heart weight to LW was found to be the highest in the F-treated groups. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), and total oxidant status (TOS) levels in the F-treated groups were significantly higher, whereas the increase in total cholesterol levels was insignificant than those in the control group. In the EGCG+F group, AST, total cholesterol, and TOS levels decreased to a level comparable to those in the control group. Histopathological evaluation revealed that there were mild changes in the portal region in the EGCG+F group; additionally, there was an improvement in liver morphology in the EGCG+F group compared to that in the F group. Thus, EGCG has potent antioxidant and regenerative effects that can ameliorate the detrimental effects of fluoride toxicity on blood parameters and the liver.Öğe Evaluating the effects of different silage additives on silage quality and in vitro digestion values of the silages of leguminous and gramineous forage plants grown without fertilizer and irrigation in central Anatolian arid conditions(Tubitak Scientific & Technological Research Council Turkey, 2021) Kara, Kamil; Kara, Kanber; Erol, Taskin; Sen, Gokhan; Karsli, Mehmet AkifThe objective of this study was to determine the effects of molasses, and bacterial inoculants on silage quality, fermentation characteristics, nutrient contents, and in vitro digestibility of different forage species grown at terrestrial climate of Central Anatolia without any artificial fertilizer usage and irrigation. Forage peas (Pisum arvense L.), Hungarian vetch (Vicia pannonica Crantz), rye grass (Lolium multiflorum Lam.) and triticale (xTriticosecale Wittmack) harvested at the dough stage of triticale and conserved in 1.5 kg jars. Silages were treated with no additive (control silage), 5% molasses and 10 g/t bacterial inoculant. Sensory, pH, organic acid, chemical analyses and in vitro digestibility of all silages were determined Forage peas silage had the highest lactic and acetic acid concentrations among all silages. Addition of both molasses and inoculant did not affect the lactic acid (LA) contents of silages (p 0.05), but both them increased acetic acid contents (p < 0.05). Ammonia-N concentrations were higher in forage peas silage compared with other silages (p < 0.05). The concentrations of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF) and crude protein (CP) were significantly different among silages (p < 0.05). The addition of molasses significantly reduced the silage OM, NDF and ADF contents (p < 0.05). In vitro OM digestibilities and energy values of silage were significantly different among silages made from different forages (p < 0.05), but not affected by silage additives. It can be concluded that high quality silage can be prepared from legume forages such as peas and vetch and small cereal grains such as rye and triticale grown without fertilizer usage and irrigation in central Anatolian arid conditions without any silage additive application, and but silage additive use may improve silage quality.Öğe Investigation of Some Morphological Traits of Boer x Hair F1 Crossbred and Pure Hair Goat Kids Raised in Semi-Intensive Conditions(Soc Chilena Anatomia, 2017) Bolacali, Memis; Ozturk, Yahya; Yilmaz, Orhan; Kucuk, Mursel; Karsli, Mehmet AkifThe aim of the study was to determine various morphological characteristics from birth to the age of 150th day of Boer x Hair goat F1 crossbred (BH) kids and pure Hair goat (HG) kids raised in semi-intensive conditions and reveal factors such as the effects of genotype, dam age, dam's live weight, year, birth type and sex on this parameter. This study was applied between the years 2011-2012 in epsilon atak in the Van region and between the years 2013-2014 at the Yuzuncu Yil University Research and Practice Farm. Various body measurements; wither height, back height, rump height, sacrum height, body length, chest length, chest depth, chest circumference, circumference of leg, cannon bone circumference, chest width, front-rump width, mid-rump width were determined 29.8, 28.4, 28.7, 26.2, 28.7, 17.0, 13.3, 30.4, 26.9, 5.2, 5.9, 6.0, and 8.7 cm at birth, respectively; 43.8, 43.8, 44.6, 40.6, 43.6, 22.2, 19.3, 51.7, 41.4, 6.6, 9.2, 9.3, and 11.3 cm at 60th day, respectively; 56.1, 53.4, 54.4, 47.5, 53.3, 27.3, 24.1, 61.4, 49.6, 7.3, 10.6, 11.1, and 13.2 cm at 150th day, respectively for BH kids; 27.3, 25.9, 29.3, 25.3, 27.1, 15.9, 12.3, 29.0, 21.3, 5.1, 5.0, 5.6, and 7.4 cm at birth, respectively; 40.4, 40.1, 42.9, 37.6, 40.5, 22.3, 18.4, 48.1, 36.4, 6.0, 7.9, 9.1, and 10.8 cm at 60th day, respectively; 50.3, 49.2, 51.0, 45.7, 50.4, 27.5, 22.9, 57.3, 43.4, 6.5, 9.5, 10.7, and 11.9 cm at 150th day, respectively for HG kids. When all of the data was considered, it was concluded that the BH kids are much better than the HG kids for both morphological and zoometric values.Öğe Performance and Some Blood Biochemical Parameters of Broilers Fed Diets Containing(Gorgan Univ Agricultural Sciences & Natural Resources, 2024) Demirci, Mehmet; Evci, Sevket; Karsli, Mehmet Akif; Aydogan, IlkayThis study aimed to explore the impact of incorporating hemp seed oil (Cannabis sativa L.) at 1.5% and 3% levels in broiler diets on broiler performance, carcass yield, weights of some internal organs, and some blood parameters. In total, 132 day-old broiler chicks (Ross 308) were utilized, with the control group (C) receiving a basal diet without supplementation. Experimental groups included 1.5% hemp seed oil (HOA) and 3% hemp seed oil (HOB) added to the basal diet. The 42-day experiment concluded with the total average live weight (LW) for control, HOA, and HOB groups reaching 2637.90, 2647.81, and 2665.68 g, respectively. Average live weight gains (LWG) were 2595.03, 2605.16, and 2622.54 g; average feed intake (FI) amounted to 4044.25, 3880.78, and 3900.36 g, and feed conversion ratio (FCR) values were 1.56, 1.50, and 1.49, respectively. While LW, LWG, and FI values did not differ significantly between groups (P > 0.05), the addition of hemp seed oil notably improved FCR (P < 0.05). Relative carcass rates and weights of carcass, heart, pancreas, and spleen were similar across groups (P > 0.05). However, liver weight was lowest in the HOA group, and the bursa of Fabricius weight was lowest in the C group (P < 0.05). No statistical differences were observed in serum glucose, albumin, triglyceride, and total cholesterol parameters among groups (P > 0.05). Total protein, HDL-c, nonHDL-c, LDL-c, TAC, and TOC values were significantly influenced by hemp seed oil (P < 0.05). In conclusion, the data suggest that adding up to 3% hemp seed oil to broiler diets can have notable effects on feed conversion ratio and serum biochemical parameters, offering critical health benefits, particularly in improving serum lipid profiles. However, the antioxidant properties of hemp seed oil were found to be weak.Öğe The effect of microbial inoculants and molasses on quality and in vitro digestibility of silages prepared with different proportions of ryegrass and Hungarian vetch(Tubitak Scientific & Technological Research Council Turkey, 2022) Sen, Gokhan; Erol, Taskin; Kara, Kamil; Demirci, Mehmet; Karsli, Mehmet AkifIn this study, it was aimed to determine the effect of microbial inoculants and molasses on the quality and in vitro digestibility of the silages ensiled from the cultivation of different proportions of ryegrass-Hungarian vetch mixtures grown in Central Anatolian conditions. For this purpose, 5% molasses and 10 g/ton (1.25 x 10(11) CFU/g) inoculant were added to silages of ryegrass (Lolium multiflorum L.) containing 20%, 40%, 60%, and 80% Hungarian vetch (Vicia pannonica L.), under laboratory conditions. The prepared silages were opened at the end of 60 days and their physical analysis, chemical contents, fermentation parameters, in vitro digestibilities using rumen inocula obtained from cannulated Holstein cow, and energy levels were determined. In the study, it was determined that the microbial inoculant increased the lactic acid level significantly (p < 0.05). In vitro digestibility of organic matter and energy values increased in silages containing 60% HV compared to other silages and with both additives (p < 0.05). The neutral detergent fiber levels increased in mixtures containing 40% and 60% Hungarian vetch (p < 0.05). The increase in the vetch ratio affected the external appearance of the silages negatively (p < 0.05), and the physical properties were adversely affected by the inoculant (p < 0.05). As a result, it was determined that Hungarian vetch can be mixed with ryegrass up to 80% with and without inoculant and molasses additive to obtain high-quality silage. But the highest digestibility values were obtained when Hungarian vetch was mixed at a 60% level. It would be more appropriate to prefer molasses to avoid undesired changes in physical properties.Öğe The effect of Saccharomyces cerevisiae as a probiotic on the nutrient degradability of some commonly feedstuffs used in Turkey(Tubitak Scientific & Technological Research Council Turkey, 2023) Hassan, Muslimo Abdullahi S. H. E. I. K. H.; Karsli, Mehmet AkifThe objective of this study was to evaluate the effects of Saccharomyces cerevisiae as a probiotic on in situ ruminal DM, OM, CP and starch degradability values in some energy and protein sources commonly used in ruminant nutrition. In this study, the in situ degradation kinetics and fractions of dry matter, organic matter, crude protein, and starch of 6 different feedstuffs (barley, wheat, corn, sunflower seed meal, cottonseed meal and soybean meal) commonly used in animal nutrition were determined. Three ruminally cannu-lated Bafra sheep were used in the experiment. The experiment was designed as carryover experimental design with two periods. Each period of the experiment, samples were incubated for 0, 2, 4, 6, 8, 12, 24, and 48 h in the rumen of 3 sheep in duplicate. Degradation kinetics and fractions of DM, OM, CP, and starch were calculated. During the entire incubation period, the DM, OM, CP, and starch degradabilities and fractions of the feedstuffs significantly differed (p < 0.01), and the feed probiotic interaction was observed for all incubation hours for the ruminal DM, OM, CP, and starch degradabilities (p < 0.04). While the DM, OM, and CP degradabilities, except 48-h incubation, were affected by probiotic supplementation of diet, starch degradabilities were affected by probiotic supplementation of diet at all incubation hours. While the potentially degradable starch fractions of the feedstuffs generally increased due to the use of probiotics, it was observed that the nondegradable fractions significantly decreased (p < 0.01). It can be concluded that Saccharomyces cerevisiae significantly increased the ruminal DM, OM, CP degradations within the 12-h incubation period of all feeds, except corn. It was observed that the addition of Saccharomyces cerevisiae into diet increased the starch degradation values in the starch-rich cereal grains and soybean meal and affected starch fractions, especially by decreasing the water-soluble and nondegradable starch fractions and increasing the potentially degradable starch fraction.Öğe Utilizing surplus bread as an ingredient in dog food: Evaluating baking and extrusion processing on physicochemical properties and in vitro digestibility performance(Academic Press Ltd- Elsevier Science Ltd, 2023) Mevliyaogullari, Ercan; Karsli, Mehmet Akif; Mert, BehicThis study probed the effects of integrating surplus bread crumbs (BC) into dog food formulations, examining the resulting influence on the rheological, physicochemical, and in vitro digestibility properties of the products processed using baking and extrusion methods. Increasing BC concentration led to increased mean cold viscosity values, decreased peak and paste viscosity, higher expansion indices, and lower bulk density. Lightness of the samples (L* values) increased significantly with higher BC ratios, resulting in lighter-colored products. The hardness of the extrudates ranged between 23.1 N and 49.4 N, and decreased with increasing BC ratios. This suggested changes in structural properties due to the addition of BC. Microstructural analysis using scanning electron microscopy demonstrated a more homogeneous matrix in BC containing samples, possibly due to in-teractions between the gelatinized starch and denatured gluten proteins from the BC. The in vitro apparent di-gestibility coefficients showed no significant variation in dry matter digestibility across BC ratios, while crude protein digestibility decreased and nitrogen-free extract digestibility increased with higher BC ratios. Specific Mechanical Energy (SME) values were found to be lower in BC-containing samples (p<0.05), indicating potential energy-saving benefits during the production process.
