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Öğe Determination of contamination sources during the manufacturing of yoghurt(Scientific Technical Research Council Turkey, 2004) Kasimoglu, A; Sireli, UT; Akgun, SThis study aimed to investigate the critical control points for the contamination in a factory experiencing a problem with coliform bacteria and Escherichia coli, and to prevent economic losses and also to protect public health by facilitating production under hygienic conditions. Samples were taken from a total of 20 control points, which involved every stage of production from the beginning to the end, including milk, all equipment, surfaces, air, hands of staff and water; yoghurt samples were taken on the same day. Microbiological analysis of all samples was performed to determine total mesophilic viable count, micrococci-staphylococci, coagulase positive staphylococci, enterobacter, coliform bacteria, E. coli, enterococci, yeast and moulds. The evaporators, milk pump, and hands of staff were determined to be critical control points for enterobacter and coliform bacteria, particularly E. coli, in the factory, but there was no risk after the training and checking of the personnel for hygiene. It was shown that factories having various contamination sources were inevitable because of the unstandardized design of milk factories in Turkey. It is necessary to determine critical control points in all factories and organized auto-control systems in order to eliminate or at least minimize the risks.Öğe Isolation and identification of motile Aeromonas spp. in ground beef(Scientific Technical Research Council Turkey, 2000) Kuplulu, O; Sarimehmetoglu, B; Kasimoglu, AIn this study, a total of 100 ground beef samples purchased at different butchers' shops and supermarkets in Ankara including 50 samples obtained from butchers' shops and 50 samples obtained from supermarkets, were analysed for the presence of motile Aeromonas species. According to the analysis, motile Aeromonas spp. were found in 73 % (73/100) of ground beef samples. Aeromonas spp. were isolated from 33 samples (66 %) obtained from butchers' shops, and 40 samples (8D %) obtained From supermarkets. A. hydrophila was the most prevalent species, followed by A. sobria and A. caviae from all of the samples. In conclusion, it was determined that ground beef samples were contaminated with motile Aeromonas species and also A. hydrophila and A. caviae as, potential pathogens, were frequently identified species. Thus, it was suggested that hygienic precautions be applied and especially ground beef should be consumed after a short storage period in a refrigerator, in homes and markets.Öğe Probiotic white cheese with Lactobacillus acidophilus(Elsevier Sci Ltd, 2004) Kasimoglu, A; Goncuoglu, M; Akgun, SThe objective of the present study was to determine the effects of Lactobacillus acidophilus on the sensory attributes, ripening time, and composition of Turkish white cheese and to investigate the survival of L. acidophilus during ripening of the cheese stored in vacuum or in brine. Two types of white cheeses, traditional cheese (control, made with Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris) and probiotic cheese (made with Lactococcus lactis ssp. lactis, Lactococcus lactis ssp. cremoris and L. acidophilus 593 N), were produced and ripened in vacuum pack or in brine at 4degreesC for 90 days. Cheese samples were assessed for microbiological and compositional properties, proteolysis, and sensory evaluation at different ripening stages. On ripening in vacuum pack, L. acidophilus survived to numbers > 10(7) cfu g(-1), Which is necessary for positive effects on health. Protein, dry matter, salt content, and percentage of lactic acid in the vacuum-packed and brine-salted probiotic cheeses were significantly different. Also, the lactic acid content of probiotic cheeses was slightly higher than that of the controls for both vacuum- and brine-packed cheeses. Vacuumpacked probiotic cheese had the highest levels of proteolysis and the highest sensory scores of all cheeses. Consequently, L. acidophihis could be used for the manufacturing of probiotic white cheese to shorten ripening time and vacuum packaging is the preferred storage format. (C) 2004 Elsevier Ltd. All rights reserved.Öğe Survival of Escherichia coli O157 : H7 in the processing and post-processing stages of acidophilus yogurt(Blackwell Publishing Ltd, 2004) Kasimoglu, A; Akgun, SIn this study the survival of Escherichia coli O157:H7 in the production process of acidophilus yogurt was examined and compared with traditional yogurt. Milk was inoculated with different doses of E. coli O157:H7 (10(2), 10(4) and 10(6) CFU mL(-1)) and two kinds of yogurt were produced. Samples were taken for pH measurements and bacterial enumeration at 0, 3, 24, 48, and 72 h after inoculation. Escherichia coli O157:H7 was analysed by using a Most Probable Number technique and yogurt starter culture and Lactobacillus acidophilus were counted by using classical culture methods. Elimination times of E. coli O157:H7 were determined for 10(2) CFU mL(-1) as 3 h, and for 10(4) and 10(6) CFU mL(-1) as 48 h in acidophilus yogurt. Elimination times in traditional yogurt were 48 h for 10(2) and 10(4) CFU mL(-1) and 72 h for 10(6) CFU mL(-1) E. coli O157:H7. In conclusion, the elimination time of E. coli O157:H7 in acidophilus yogurt was shorter than in traditional yogurt during the processing and post-processing stages.
