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  • Küçük Resim
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    The protective effects of dexmedetomidine on hepatic ischemia reperfusion injury
    (Comenius University, 2014) Küçük, A.; Yaylak, F.; Cavunt-Bayraktar, A.; Tosun, M.; Arslan, M.; Çomu, F.M.; Kavutcu, M.
    Objective: The aim of this study was to evaluate the effect of dexmedetomidine (100 ?g/kg-ip) on liver ischemia and reperfusion (I/R) in rats. Methods: Twenty-four Wistar Albino rats were separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 minutes and reperfusion period was 45 minutes after declampage. Group I/R-D was received dexmedetomidine 100 ?g/ kg i.p. 30 min before portal clampage. Thiobarbutiric Acid-Reactive Substances (TBARS), glutathioneS-transferase (GST), superoxide dismutase (SOD), Catalase (CAT), and Paraoxonase 1 (PON-1) were investigated in blood samples. Also HSP60 and p53-positive hepatocytes were counted under ImageJ image analysis program. Results: All parameters, except GST levels, were significant between the groups (p < 0.05). Although HSP60 expression was significantly increased between I/R, I/R-D and C groups there were no significant differences between I/R-D and C (p = 0.443). On the other hand, p53 expression was also significantly increased between I/R, I/R-D and C groups At the same time, there were no significant differences between I/R-D and C groups (p = 0.354). Conclusion: All the results suggest that dexmedetomidine has beneficial effects on liver ischemia/reperfusion stress.
  • Küçük Resim
    Öğe
    Vitamin C ameliorates high dose Dexmedetomidine induced liver injury
    (Comenius Univ, 2016) Arslan, M.; Sezen, S. C.; Turgut, H. C.; Kocabiyik, M.; Arpaci, H.; Comu, F. M.; Kavutcu, M.
    BACKGROUND: We investigated whether vitamin C has protective effects on rat liver tissue treated with different dexmedetomidine doses. MATERIAL AND METHODS: Thirty five wistar albino rats were randomly divided into 5 groups (Control (0.9 % NaCI intraperitoneally (ip), Dexmedetomidine 5 mu g.kg(-1) (ip), Dexmedetomidine 5 mu g.kg(-1) ip plus Vitamin C (100 mng.kg(-1)), Dexmedetomidine 10 mu g.kg(-1) ip and Dexmedetomidine 10 mu g.kg(-1) ip plus Vitamin C (100 mg.kg-1). Histopathological liver injury, superoxide dismutase (SOD) activity and tissue Malondialdehyde levels were investigated. RESULTS: Hepatocyte degeneration was significantly higher in D10 group than those in other study groups (p < 0.0001, p = 0.002, p < 0.0001, p = 0.005, respectively). Similarly, liver tissue sinusoidal dilatation and hepatocyte necrosis were significantly higher in D10 group than those in other groups (p < 0.0001, p < 0.0001, p = 0.002, p < 0.0001 and p < 0.0001, p = 0.046, p < 0.0001 and p = 0.002, respectively). Tissue MDA levels in D10 group were significantly higher than those in control, D5+Vit C and D10+Vit C groups (p = 0.028, p = 0.004, p = 0.031, respectively). SOD enzyme activity in D10 group was significantly lower than in control, D5+Vit C and D10+Vit C groups (p < 0.0001, p = 0.023 and p = 0.031, respectively). CONCLUSION: High dose dexmedetomidine can induce hepatic injury and oxidative stress in rats while pretreatment with vitamin C may be effective in protecting liver tissue against this newly recognized undesirable dexmedetomidine effect (Tab. 2, Fig. 5, Ref. 30). Text in PDF www.elis.sk.