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    Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa
    (Akademiai Kiado Zrt, 2021) Kabakci, Ruhi; Kaya, Abdulkadir; Yiğit, Ayşe Arzu; Varisli, Ömer
    This study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001-100 mM) were evaluated at different incubation times (0-6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from the parenchyma of bovine testes, were seeded at 1.0 x 10(4) and 1.5 x 10(6) cells/well in 96- and 12-well culture plates and incubated for 48 h in culture media containing TEB (0.001- 100 mM) to evaluate cytotoxicity and hormone release, respectively. TEB did not affect the motility and plasma membrane integrity. However, significant spermiotoxicity occurred at higher TEB (1-100 mM) concentrations (P < 0.05) compared to control and lower doses. Although no dose caused cytotoxicity in testicular cells (P > 0.05), 1 and 100 mM TEB caused a significant increase in testosterone secretion (P < 0.05). As a result, high doses of TEB (1-100 mM) had slightly suppressive effects on spermatozoa; however, these doses had stimulatory effects on testosterone secretion by testicular cells. It appears that the disruption of hormonal homeostasis of testicular cells after TEB exposure may result in metabolic and especially reproductive adverse effects in bulls.
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    Öğe
    IN VITRO SPERMATOLOGICAL PARAMETERS IN DRONES
    (Bursa Uludag University, 2023) Kaya, Abdulkadir; Uysal, Ongun
    Honey bees are an indispensable element of the ecosystem, as they provide an important part of plant pollination beyond food production. The queen bee, which forms the core of the bee colony, is at the center of bee production in the beekeeping sector. Undoubtedly, the production of high-quality queen bees primarily depends on suitable drones and therefore, quality sperm. In addition, artificial insemination of queen bees is a successful production method, as in other species, due to its superior characteristics compared to natural mating. In many apiaries and research centers, artificial insemination is used routinely for queen production. One of the advantages of this method is that the sperm can be preserved for the short or long term. In this respect, it is very effective in protecting genetic resources, preventing bee diseases and facilitating bee transportation. Today, spermatological studies and parameters used in drones are very few compared to other species. With the increasing importance given to the subject in recent years, many spermatological parameters have started to be used in honey bees. However, the morphological and physiological differences of drone semen require the development and standardization of these parameters with further studies. In this review, the spermatological parameters used in drone semen, together with the working principles and materials used, were examined in general terms and presented to the readers. © 2023 Authors. All rights reserved.