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Öğe Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro(Academic Journals, 2011) Turk, Mustafa; Kaya, Bulent; Menemen, Yusuf; Oguztuzun, SerpilApoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla on HeLa cells were investigated. The ratio of viable cells was determined by cell counter. Double staining method for the determination of apoptotic and necrotic index was carried out. The lowest apoptotic effect was found in Alchemilla oriturcica with the ratio of around 14% and the highest apoptotic effect found in Alchemilla trabzonica was 24%. Apoptotic effect was additionally determined by caspase-3 immunostaining. Increasing the concentrations of the extracts, especially in the level of 150 to 200 mu g/mL caused the increase of necrotic effect on cancer cells. Particularly, the effects of A. oriturcica and A. trabzonica extracts with the density of 200 mu g/mL were very high compared to the other species. The flavonoid components were detected in extracts of the species used for this study. The flavonoids identified are orientin (luteolin-8-C-glucoside), hyperoside (quercetin-3-O-galactoside) and isoquercetin (quercetin-3-glucoside) in Alchemilla erythropoda; rutin (quercetin-3-rutinoside), orientin, vitexin (apigenin-8-C-glucoside), hyperoside in Alchemilla ikizdereensis; rutin, hyperoside, isoquercetin (quercetin-3-glucoside) in A. oriturcica, and hyperoside, isoquercetin and quercitrin (quercetin-3-O-rhamnoside) in A. trabzonica.Öğe Flavonoid compounds identified in Alchemilla L. species collected in the North-eastern Black sea region of Turkey(African Networks Ethnomedicines, 2012) Kaya, Bulent; Menemen, Yusuf; Saltan, Fatma ZerrinThis study identified flavonoid glycosides in species of the genus Alchemilla, A. procerrima, A. stricta, A. hirtipedicellata and A. sericata. A. procerrima is an endemic species for Turkey. After detailed investigation, flavonoid compounds of the species were identified for the first time. In this study, flavonoid compounds were determined by using two different chromatography techniques, TLC and HPLC. The following flavonoid compounds were identified from the Alchemilla species studied. They are as follows: orientin (luteolin-8-C-glucoside) Rf: 0,70, vitexin (apigenin-8-C-glucoside) Rf: 0,77 as flavone-C-glycoside, rutin (quercetin-3-O-rutinoside) Rf: 0,44, hyperoside (quercetin-3-O-galactoside) Rf: 0,65, isoquercetin (quercetin-3-O-glucopyranoside) Rf: 0,72, quercitrin (quercetin-3-O-rhamnoside) Rf: 0,84 as flavonol-O-glycoside. Three more folavonoids with Rf values of Rf(1)= 0,36, Rf(2)=0,54 and Rf(3)=0,68 were also identified for the first time in this study. Rutin (quercetin-3-O-rutinoside) and the flavonoid glycoside, shown as Rf(2) were found in all species. Quercitrin and isoquercetin were determined in all analysed species but A. procerrima. Hyperoside was identified in all species except for A. stricta. Vitexin was determined only in A. stricta. Orientin was determined in A. procerrima and A. stricta, but could not be determined in A. sericata and A. hirtpedicellata. Unknown flavonoid with Rf(1) and Rf(3) were determined outside of A. sericata. Description of these compounds in Turkish Alchemilla plants for the first time should be viewed as a discovery of an important chemosystematic feature.Öğe Flavonoids in the endemic species of Alchemilla L., (section Alchemilla L. subsection Calycanthum Rothm. Ser. Elatae Rothm.) from north-east black sea region in Turkey(Pakistan Botanical Soc, 2012) Kaya, Bulent; Menemen, Yusuf; Saltan, F. ZerrinThis study was undertaken to determine the flavonoids in the species of genus Alchemilla L. section Alchemilla L. subsection Calycanthum Rothm. Ser. Elatae Rothm., using the identification techniques such as high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). Eight endemic Turkish species were studied for their flavonoid profiles. They are A. armeniaca, A. erzincanensis, A. cimilensis, A. orduensis, A. ikizdereensis, A. oriturcica, A. bursensis and A. hirsutiflora. It is the first time that flavonoid profiles of these species have been identified. Flavonol O-glycosides and flavone C- glycosides were identified in Alchemilla endemic species. The flavonoid components determined in the species are rutin (quercetin-3-O-rutinoside), hyperoside (quercetin-3-O-galactoside), isoquercetin (quercetin-3-O-glucopyranoside), quercitrin (quercetin-3-O-rhamnoside) as flavonol O-glycosides, and orientin (luteolin-8-C-glucoside), vitexin (apigenin-8-C-glucoside) as the flavone C-glycosides.Öğe Magnetite Fe3O4 nanoparticles impregnated on Chlorella vulgaris microalgae: Its role in obtaining hydrogen from the sodium borohydride-hydrolysis(Elsevier, 2024) Duman, Sibel; Kaya, Bulent; Caf, Fatma; Kok, Osman; Tuzun, IlhamiRecently, the single-celled green freshwater microalgae species Chlorella vulgaris has attracted the attention of researchers due to its different usage areas. In particular, research focuses on the technology of obtaining bio-hydrogen with various techniques. This research involves, for the first time, the use of the microalga Chlorella vulgaris as a bio-supporting material for magnetite Fe3O4 3 O 4 nanoparticles (Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris) ) and the production of hydrogen through catalytic hydrolysis of NaBH4 4 (sodium borohydride, SB) in the presence of the resulting magnetite nanoparticles. Here, detailed kinetic studies were carried out during the SB-hydrolysis by taking magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris and SB in varying amounts and at varying temperatures, and the activation energy and lifetime of magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris was found to be 23.49 kJ mol-1- 1 and 93,280 mol H2 2 (mol Fe3O4)-1 , 3 O 4 )- 1 , respectively. No change in the chemical and physical structure of the biocatalyst was observed during the hydrolysis of SB, so only detailed characterization of microalgae and magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris was performed, and the particle size of the catalyst was calculated as 10.19 +/- 2.17 nm. The results showed that these Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris , , which can be easily separated magnetically and have high catalytic activity, are a clean and quite surprising catalyst in terms of hydrogen production.Öğe Novel phthalocyanines containing resorcinol azo dyes; synthesis, determination of pKa values, antioxidant, antibacterial and anticancer activity(Elsevier Science Sa, 2015) Kantar, Cihan; Akal, Hayriye; Kaya, Bulent; Islamoglu, Fatih; Turk, Mustafa; Sasmaz, SelamiThere are many studies about effects on health of compounds having azo groups in literature. However, anticancer, antimicrobial and antioxidant properties of phthalocyanines containing azo dyes are unknown. Therefore, the new metallophthalocyanines (M: Mn, Co, Zn) substituted with resorcinol azo dyes were synthesized and anticancer, antimicrobial and antioxidant properties investigated. All phthalocyanines were synthesized by the microwave assisted method. The structures were characterized by elemental analysis, H-1 NMR, C-13 NMR, UV/vis, IR and Mass spectroscopy techniques. The antioxidant capacities were analyzed through radical scavenging of DPPH and chelating ability to ferrous cation. The highest DPPH activity was obtained from compounds (1c and 2c) and the best ferrous ion chelating activity was determined from compound 2. The antibiotic activities were evaluated by disc diffusion method. Compound 2 exhibited antibacterial activity against studied bacteria, but the other compounds had no significant antibacterial effect. Cytotoxicity, apoptotic and necrotic effects were examined on cancer cells MCF-7 and healthy fibroblast cells. Compounds 1, 1a and 2a seem to be proper for the future cancer therapy researches. pKa values were determined by potentiometrically according to the half-neutralization method in N,N-dimethyl formamide. The effect of pH on the electronic absorption spectra of compounds (1, 2, 1c and 2c) was reported. The newly synthesized compounds show positive solvatochromism behavior. (C) 2015 Elsevier B.V. All rights reserved.
