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  • Küçük Resim
    Öğe
    The detection of Brucella spp. by BACTEC 9050 blood culture system
    (2004) Ayaşlioğlu, Ergin; Kiliç, Dilek; Kaygusuz, Sedat; Küçük, Seda; Çeken, Sebahat; Erol, Özlem; Alpay, Yeşim
    Recent reports have demonstrated that automated continous monitoring blood culture systems are fast and efficent in the detection of Brucella spp. In this retrospective study, the detection of these slow-growing bacteria by BACTEC 9050 blood culture system was evaluated. For this purpose, 60 patients, whose blood cultures were monitored by using BACTEC 9050 system were included into the study. Brucella spp. were isolated in 26 of 31 patients from whom two blood cultures were obtained and in 17 of 29 patients from whom single blood culture were obtained. The majority of isolates (84.1 %) were detected within 7 days of incubation while the earliest detection was on the 3rd day in two samples. However, the bacteria were isolated by subcultures after 30 days of incubation in 8 of the samples. In conclusion, the routine 5 or 7 days-incubation protocols with BACTEC 9050 system were not efficient for the isolation of Brucella spp. Obtaining two blood cultures and prolonge incubation followed by subcultures increased the probability of bacterial isolation.
  • [ X ]
    Öğe
    Diagnosis of acute brucellosis by polymerase chain reaction technique
    (Refik Saydam National Public Health Agency (RSNPHA), 2015) Çeken, Sabahat; Kaygusuz, Sedat; Kiliç, Dilek; Ağalar, Canan
    Objective: Brucellosis is a zoonotic disease which is a public health problem in our country like many parts of the world. The illness has nonspecific symptoms and physical signs. Bacterial culture is gold standard in the diagnosis of brucellosis but it is difficult and time consuming, so serologic techniques are used routinely. But serologic techniques have low specificity because of cross reactions. Molecular methods like polymerase chain reaction (PCR) are good alternatives in the early and rapid diagnosis of brucellosis, as it is in other infectious diseases. The aim of this study was to compare PCR method with conventional methods in the diagnosis of brucellosis. Methods: The study included 35 patients and 20 healthy volunteers. Sera for serology and whole blood samples for PCR were collected from each subject in both groups. DNA was extracted from peripheral mononuclear cells obtained from the blood samples and an in house PCR assay was used to detect brucella DNA. Results: Standart tube agglutination (STA) titers of most patients were ?1/160, except one patient which was 1/80. Blood cultures were positive for 16 patients. The STA titers of all controls were negative. PCR was positive for 97% of the patients and all of the volunteers were negative. Conclusion: It is concluded that the tested PCR assay has high sensitivity in the diagnosis of brucellosis and it may be used in rapid and accurate diagnosis of brucellosis.
  • [ X ]
    Öğe
    The detection of Brucella spp. by BACTEC 9050 blood culture system
    (2004) Ayaşlio?lu, Ergin; Kiliç, Dilek; Kaygusuz, Sedat; Küçük, Seda; Çeken, Sebahat; Erol, Özlem; Alpay, Yeşim
    Recent reports have demonstrated that automated continous monitoring blood culture systems are fast and efficent in the detection of Brucella spp. In this retrospective study, the detection of these slow-growing bacteria by BACTEC 9050 blood culture system was evaluated. For this purpose, 60 patients, whose blood cultures were monitored by using BACTEC 9050 system were included into the study. Brucella spp. were isolated in 26 of 31 patients from whom two blood cultures were obtained and in 17 of 29 patients from whom single blood culture were obtained. The majority of isolates (84.1 %) were detected within 7 days of incubation while the earliest detection was on the 3rd day in two samples. However, the bacteria were isolated by subcultures after 30 days of incubation in 8 of the samples. In conclusion, the routine 5 or 7 days-incubation protocols with BACTEC 9050 system were not efficient for the isolation of Brucella spp. Obtaining two blood cultures and prolonge incubation followed by subcultures increased the probability of bacterial isolation.