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    The Effect of Hydrogen-Rich Saline Solution on Erythrocyte Deformability in Lower Limb Ischemia Reperfusion Injury in Rats
    (Gazi Univ, Fac Med, 2021) Ozer, Abdullah; Arslan, Mustafa; Comu, Faruk Metin; Kucuk, Aysegul; Mardin, Baris; Kocak, Basak; Oktar, Gursel Levent
    Aim: We aimed to investigate the effects of HRSS on erythrocyte deformability in lower limb ischemia reperfusion (IR) injury in rats. Methods: Eighteen male Wistar albino rats used in this study. The animals were randomly divided into three experimental groups as control, IR and IR-HRSS. 20 mg.kg-1HRSS was administered (20 mg.kg(-1) i.p)30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. 120 min ischemia and 120 min reperfusion is applied to the groups. Erythrocytes were obtained from heparinized whole blood samples for deformability measurements. Kruskal-Wallis test was used for independent samples and Mann-Whitney U test was used to analyze differences between groups. Results: Ischemia reperfusion was found to increase relative resistance to the control group. The erythrocyte deformability index was significantly higher in IR and IR-HRSS groups than the control group. HRSS application significantly decreased erythrocyte deformability index compared to IR group. Conclusion: IR induced rats decreased erythrocyte deformability was partially corrected by HRSS. We believe that the protective effects of HRSS in IR injury and its use indications can be demonstrated in detail as long as the findings we have reached in our study are supported by other studies.
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    The Effect of Proanthocyanidin on Ischemia-Reperfusion Injury in Skeletal Muscles of Rats
    (Mdpi, 2024) Ozer, Abdullah; Kocak, Basak; Sezen, Saban Cem; Arslan, Mustafa; Kavutcu, Mustafa
    Background and Objectives: Lower limb skeletal muscle ischemia-reperfusion (IR) injury is associated with increased morbidity and mortality, and it is common in several clinical situations such as aortic aneurysms repairment, peripheral arterial surgery, vascular injury repairment, and shock. Although it is generally accepted that oxidative stress mediators have a significant role in IR injury, its precise mechanism is still unknown. Anecdotally, it is sustained not only by structural and functional changes in the organ it affects but also by damage to distant organs. The purpose of this report is to illustrate the effect of proanthocyanidin on IR injury. Materials and Methods: In our study, 18 male Wistar albino rats were used. The subjects were divided into three groups containing six mice each (control, C; ischemia-reperfusion, IR; ischemia-reperfusion and proanthocyanidin; IR-PRO). Intraperitoneal proanthocyanidin was given to the IR and proanthocyanidin groups 30 min before laparotomy, and 1 h ischemia led to these two groups. After one hour, reperfusion started. Muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, muscle oval-central nucleus ratio, leukocyte cell infiltration, catalase enzyme activity, and TBARS were all examined in lower-limb muscle samples after one hour of reperfusion. Results: When skeletal muscle samples were evaluated histopathologically, it was discovered that muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, and leukocyte cell infiltration with oval-central nucleus standardization were significantly higher in the IR group than in the C and IR-P groups. Oval-central nucleus standardization was significantly higher in the IR and IR-PRO groups than in the control group. TBARS levels were significantly higher in the IR group than in the control and IR-PRO groups, while catalase enzyme activity was found to be significantly lower in the IR group than in the control and IR-PRO groups. Conclusions: As a consequence of our research, we discovered that proanthocyanidins administered before IR have a protective impact on skeletal muscle in rats. Further research in this area is required.
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    Öğe
    The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats silymarin and ischemia-reperfusion injury
    (Turkish National Vascular and Endovascular Surgery Society, 2024) Ozer, Abdullah; Kocak, Basak; Arslan, Mustafa; Mardin, Baris; Kucuk, Aysegul; Sezen, Saban Cem; Zor, Mustafa Hakan
    Aim: Although the precise process is not entirely elucidated, it is well-known that oxidative stress mediators contribute to ischemia-reperfusion (I/R) injury. The impact of silymarin on I/R injury in many different tissues has been examined. For this purpose, we planned to see the effect of silymarin on muscle tissue in rats subjected to lower extremity I/R injury. Material and Methods: We used 18 male Wistar albino rats weighing 225-275 g. Each of the three groups of rats [Control (C), Ischemia-Reperfusion (I/R), and Silymarin-Ischemia/Reperfusion (S-I/R)] consisted of six rats. Silymarin was administered intraperitoneally 30 minutes before the procedure. (100 mg/kg-1) In Group I/R, the infrarenal abdominal aorta was clamped with a microvascular clamp. The clamp was removed after 120 minutes, and reperfusion was achieved for the next 120 minutes. At the end of the reperfusion period, muscle tissue samples were collected, and Malondialdehyde (MDA), catalase (CAT) enzyme activity levels and histopathological parameters were compared. Results: In the histopathological examination, no degeneration was observed in the muscle fibers in the Group C, while findings of striated muscle damage such as muscle atrophy/hypertrophy, muscle degeneration/congestion, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization and leukocyte cell infiltration were seen in the Group I/R. In Group S-I/R, muscle atrophy /hypertrophy, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization, and leukocyte cell infiltration were observed to improve these damaged areas compared to Group I/R. MDA levels in the Group I/R were significantly higher compared to Group C and S-I/R. The activity of the CAT enzyme was much higher in Group I/R compared to Group C. Conclusion: Our study revealed that 100 mg/kg-1 silymarin administered by intraperitoneal injection 30 minutes before ischemia effectively decreased lipid peroxidation, oxidative stress, and the injury caused by I/R in muscle histology in rats. © 2024, Turkish National Vascular and Endovascular Surgery Society. All rights reserved.