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    Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits
    (Elsevier Sci Ltd, 2013) Kayan, Autchara; Uddin, Muhammad Jasim; Kocamis, Hakan; Tesfaye, Dawit; Looft, Christian; Tholen, Ernst; Cinar, Mehmet Ulas
    The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F-2 Duroc x Pietrain (DuPi, n = 313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n = 110) population. HNF1A mRNA and protein expressions were higher (p < 0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p > 0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect. (C) 2013 Elsevier Ltd. All rights reserved.
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    Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle
    (Scientific Technical Research Council Turkey-Tubitak, 2019) Kurum, Aytul; Karahan, Siyami; Kocamis, Hakan; Cinar, Miyase; Ergun, Emel
    Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.
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    Determining Gene Expression Profile of GPX 1 in the Liver of Diabetic Rats Treated with Capsaicin by Real-Time PCR
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Kurum, Aytul; Kocamis, Hakan; Deprem, Turgay; Cinar, Mehmet Ulas
    The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis.