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Öğe Auricular cartilage repair using cryogel scaffolds loaded with BMP-7-expressing primary chondrocytes(Wiley, 2013) Odabas, S.; Feichtinger, G. A.; Korkusuz, P.; Inci, I.; Bilgic, E.; Yar, A. S.; Piskin, E.The loss of cartilage tissue due to trauma, tumour surgery or congenital defects, such as microtia and anotia, is one of the major concerns in head and neck surgery. Recently tissue-engineering approaches, including gene delivery, have been proposed for the regeneration of cartilage tissue. In this study, primary chondrocytes were genetically modified with plasmid-encoding bone morphogenetic protein-7 (BMP-7) via the commercially available non-viral Turbofect vector, with the aim of bringing ex vivo transfected chondrocytes to resynthesize BMP-7 in vitro as they would in vivo. Genetically modified cells were implanted into gelatin-oxidized dextran scaffolds and cartilage tissue formation was investigated in 15x15mm auricular cartilage defects in vivo in 48 New Zealand (NZ) white rabbits for 4months. The results were evaluated via histology and early gene expression. Early gene expression results indicated a strong effect of exogenous BMP-7 on matrix synthesis and chondrocyte growth. In addition, histological analysis results exhibited significantly better cartilage healing with BMP-7-modified (transfected) cells than in the non-modified (non-transfected) group and as well as the control. Copyright (c) 2012 John Wiley & Sons, Ltd.Öğe BMP-7 expressing genetically modified primary chondrocytes in cryogel scaffolds for rabbit auricular cartilage repair(Wiley-Blackwell, 2012) Odabaş, S.; Feichtinger, G.; Korkusuz, P.; İnci, I.; Bilgiç, E.; Yar, A. S.; Pişkin, E.…Öğe Chondroitin sulfate-coated polyhydroxyethyl methacrylate membrane prevents adhesion in full-thickness tendon tears of rabbits(W B Saunders Co-Elsevier Inc, 2002) Güdemez, E.; Eksioglu, F.; Korkusuz, P.; Asan, E.; Gürsel, I.; Hasirci, V.Polyhydroxyethyl methacrylate (pHEMA) membranes coated on one side with chondroitin sulfate (CS) were used to block adhesion physically and to reduce friction between healing flexor tendons and the surrounding tissue in rabbit forepaws after surgical repair. Digits with pHEMA-only, standard tendon sheath repair, and with no sheath repair were the controls. Over 12 weeks the CS-coated membranes were evaluated for joint flexion, adhesion limitation, and tendon healing progress. The membranes initially allowed for better flexion (i.e, for 6 weeks), but their relative superior effectiveness faded afterward. Histology showed that adhesions were less severe and healing was better in the CS-pHEMA membranes at 3 and 6 weeks. If further studies determine precise amounts or thicknesses of CS coats that will maximize its healing properties, CS-pHEMA should prove useful in clinical settings in which restoration of tendon sheath integrity with a minimum of adhesions is not possible. Copyright (C) 2002 by the American Society for Surgery of the Hand.Öğe Cryogel scaffolds with stem cells for bone tissue engineering: an animal model(Mary Ann Liebert Inc, 2008) Boelgen, N.; Vargel, I.; Cavusoglu, T.; Korkusuz, P.; Guezel, E.; Piskin, E.…Öğe Electrospun spiral-wounded polycaprolactone scaffolds with or without stem cells for cranial bone defects: an animal model(Mary Ann Liebert Inc, 2008) Isoglu, I. A.; Bolgen, N.; Vargel, I.; Cavusoglu, T.; Korkusuz, P.; Guzel, E.; Piskin, E.…Öğe Filling cranial defects in rats: 6 months animal study(Mary Ann Liebert Inc, 2008) Aydin, H. M.; Vargel, I.; Cavusoglu, T.; Korkusuz, P.; Guzel, E.; Piskin, E.[Abstract No tAvailable]Öğe In vivo performance of antibiotic embedded electrospun PCL membranes for prevention of abdominal adhesions(Wiley, 2007) Bölgen, N.; Vargel, İ.; Korkusuz, P.; Menceloglu, Y. Z.; Pişkin, E.The aim of this study was to prepare nonwoven materials from poly(epsilon-caprolactone) (PCL) and their antibiotic containing forms by electrospinning, so as to prevent postsurgery induced abdominal adhesions in rats. epsilon-Caprolactone was first polymerized by ring-opening polymerization, and then it was processed into matrices composed of nanofibers by electrospinning. A model antibiotic (Biteral(R)) was embedded within a group of PCL membranes. In the rat model, defects on the abdominal walls in the peritoneum were made to induce adhesion. The plain or antibiotic embedded PCL membranes were implanted on the right side of the abdominal wall. No membrane implantation was made on the left side of the abdominal wall that served as control. Macroscopical and histological evaluations showed that using these barriers reduces the extent, type, and tenacity of adhesion. The antibiotic embedded membranes significantly eliminated postsurgery abdominal adhesions, and also improved healing. (C) 2006 Wiley Periodicals, Inc.Öğe In vivo response to biodegradable controlled antibiotic release systems(John Wiley & Sons Inc, 2001) Korkusuz, F.; Korkusuz, P.; Ekşioğlu, F.; Gürsel, I.; Hasirci, V.In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ratio of 1:1 (w/w) were effective in treating the bone infection that was experimentally initiated by inoculation of a hemolytic strain of Staphylococcus aureus (coagulase positive; phage type 52/52b) together with metal implants into the medullary area of rabbit tibia. Macroscopical data revealed that the effectiveness of therapy was apparent at week 6 for all categories tested. Radiological findings with Duocid(R)- and Sulperazone(R)-loaded P(3-HB-co-4-HB) rods improved significantly when judged by changes in periosteal elevation, widening of bone shaft, new bone formation, and soft-tissue deformation after 6 weeks of implantation. Histologically the signs of infection were found to subside by weeks 3 and 6. inflammatory cells were replaced with bone-forming cells upon treatment with Sulperazone(R)-P(3-HB-co-4-HB) and Duocid(R)-P(3-HB-co-4-HB). Osteoblastic activity was prominent. Intramedullary inflammation, although still present, started to be replaced by fibrous or bony tissue. Histological findings presented the subsidence of infection. In summary, the antibiotic-loaded biopolymeric rods appeared to have potential as a new controlled-release system for the treatment of implant related osteomyelitis and chronic osteomyelitis. (C) 2001 John Wiley & Sons, Inc.Öğe Lactide-PEG cryostructures with stem cells as tissue engineering scaffolds in reconstruction of cranial bone defects in rat model(Mary Ann Liebert Inc, 2008) Egri, S.; Vargel, I.; Cavusoglu, T.; Korkusuz, P.; Guzel, E.; Uckan, D.; Piskin, E.…Öğe Novel biodegradable cryogels made of(Mary Ann Liebert Inc, 2007) Egri, S.; Vargel, I.; Korkusuz, P.; Plieva, F.; Galaev, I.; Piskin, E.[Abstract No tAvailable]
