Yazar "Kurtipek, Omer" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    Effects of Dexmedetomidine Administered Through Different Routes on Kidney Tissue in Rats with Spinal Cord Ischaemia-Reperfusion Injury
    (Dove Medical Press Ltd, 2022) Sengel, Necmiye; Koksal, Zeynep; Dursun, Ali Dogan; Kurtipek, Omer; Sezen, Saban Cem; Arslan, Mustafa; Kavutcu, Mustafa
    Background: Ischaemia-reperfusion (IR) injury, which can be encountered during surgical procedures involving the abdominal aorta, is a complex process that affects distant organs, such as the heart, liver, kidney, and lungs, as well as the lower extremities. In this study, we aimed to contribute to the limited literature by investigating the protective effect of dexmedetomidine, which was administered through different routes, on kidney tissue in rats with spinal cord IR injury.Methods: A total of 30 rats were randomly divided into five groups: control (C group), IR (IR group), IR-intraperitoneal dexmedetomidine (IRIPD group), IR-intrathecal dexmedetomidine (IRITD group), and IR-intravenous dexmedetomidine (IRIVD group). The spinal cord IR model was established. Dexmedetomidine was administered at doses of 100 mu g/kg intraperitoneally, 3 mu g/ kg intrathecally, and 9 mu g/kg intravenously. Histopathologic parameters in kidney tissue samples taken at the end of the reperfusion period and biochemical parameters in serum were evaluated.Results: When examined histopathologically, tubular dilatation was found to be significantly reduced in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.012, all). Vascular vacuolization and hypertrophy were significantly decreased in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.006, all). Tubular cell degeneration and necrosis were significantly reduced in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.008, p = 0.08, and p = 0.030, respectively). Lymphocyte infiltration was significantly decreased in the IRIVD and IRITD groups compared with the IR group (p = 0.006 and p = 0.06, respectively). Conclusion: It was observed that dexmedetomidine administered by different routes improved the damage caused by IR in kidney histopathology. We think that the renoprotective effects of dexmedetomidine administered intravenously and intrathecally before IR in rats are greater.
  • [ X ]
    Öğe
    Effects of esmolol on lung injury induced by lower extremity Ischemia-reperfusion
    (Bayrakol Medical Publisher, 2022) Sabuncu, Ulku; Sezen, Saban Cem; Kucuk, Aysegul; Salman, Nevriye; Sabuncu, Timucin; Kip, Gulay; Kurtipek, Omer
    Aim: After hind limb ischemia-reperfusion (I/R), impairments in remote organs are frequent. Lung tissue is the organ most affected by the remote organ damage. The lung damage increases ventilatory support, the need for inotropic agents and mortality. Many drugs and methods have been used in attempts to prevent or reduce this damage. The aim of this study is to investigate the protective effects of esmolol infusion on lung tissue prior to I/R created in the lower extremity. Material and Methods: The study was performed between 11 and 14 April 2018 in Gazi University Experimental Animal Research Center, Ankara, Turkey. After obtaining ethics committee approval, 24 rats were randomly divided into 4 groups: Control (Group C), Esmolol (Group E), Ischemia-reperfusion (Group I/R), and I/R-Esmolol (Group I/RE). Esmolol (200 mu g/kg/min intravenous) was applied 30 minutes before the procedure. The biochemical and histopathological parameters of lung tissue samples were compared. Results: Neutrophil infiltration/aggregation, alveolar wall thickness, and total lung injury scores were significantly higher in the I/R group than in the C and E groups. In addition, neutrophil infiltration/aggregation, alveolar wall thickness, and total lung injury scores in the I/R group were statistically higher than in the I/R-E group (p=0.030, p=0.010, p=0.001, respectively). Malondialdehyde levels, catalase (CAT) and paraoxonase (PON) enzyme activities in the I/R group were significantly higher than in the C, E, and I/R-E groups. Glutathione S- transferase ( GSH) enzyme activity was similar in all groups. Discussion: It was found that esmolol infusion at 200 mu g/kg/min intravenously-reduced oxidative stress when administered 30 minutes before ischemia in rats and partially corrected the damage caused by I/R in lung histopathology.
  • Küçük Resim
    Öğe
    Effects of the general anaesthetic agent, propofol, on erythrocyte deformability
    (Comenius Univ, 2010) Arslan, Mustafa; Comu, Faruk Metin; Isik, Berrin; Unal, Yusuf; Cekmen, Nedim; Kurtipek, Omer
    Objectives: Propofol is an anesthetic agent frequently used for sedation and general anesthesia. The purpose of our study was to investigate the effect of propofol, a general anesthetic, on erythrocyte deformability in rats. Methodology: The study was performed on 20 male and 20 female rats, with 10 rats in each study group and 10 rats in each control group. The rats in the study group were administered propofol (150 mg.kg(-1)) intraperitoneally, and the rats in the control group were administered SF. Erythrocyte packs were prepared using heparinized total blood samples. Deformability measurements were done by erythrocyte suspensions in PBS buffer. A constant flow filtrometer system was used to measure erythrocyte deformability, and the relative resistance was calculated. Results: The use of propofol resulted in the increase in the relative resistance, which is an indicator for the erythrocyte deformability in both male and female rats (p=0.002, p=0.042, respectively). Conclusion: A negative change in the erythrocyte deformability may cause a functional deterioration in blood flow and tissue perfusion (Fig. 1, Ref. 23). Full Text (Free, PDF) www.bmj.sk.