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  • Küçük Resim
    Öğe
    Adsorption Of Fe2+ Ions With Saccharomyces cerevisiae Immobilized Pumice Stone
    (2005) Lale, Mustafa; Şahin, Nazan; Temoçin, Zülfikar
    Bu çalışmada kolon yöntemi kullanılarak Fe{2} iyonlarının Saccharomyces cerevisiae immobilize edilmiş ponza taşına adsorpsiyon şartları araştırıldı. Bu amaçla; adsorpsiyona çözelti pH sının, iyon başlangıç derişiminin ve adsorplanan iyonların geri kazanılması için uygun çözelti türü ve derişiminin etkisi araştırıldı. Maksimum adsorpsiyonun sağlandığı pH değerinin 5 olduğu belirlendi. Optimum pH değerinde, adsorbanın metal tutma kapasitesi 60 \mu g/mL başlangıç derişiminde 1,81mg/g dır. Desorpsiyona HC1, NaCl, NH4C1 ve EDTA çözeltilerinin etkisi araştırıldı. En uygun çözeltinin 0,2 M'lık EDTA çözeltisi olduğu gözlendi. 0,2 M EDTA çözeltisi ile %98 oranında desorpsiyon sağlandı.
  • Küçük Resim
    Öğe
    Adsorption of Fe2+ IONS with Saccharomyces cerevisiae immobilized Pumice stone
    (Gazi Univ, 2005) Lale, Mustafa; Şahin, Nazan; Temoçin, Zülfikar
    In this study, the adsorption conditions of Fe2+ ions on Saccharomyces cerevisae immobilized pumice stone were investigated by column method. In order to perform this aim, the effect of solution pH value, initial concentration of ions and suitable solution and concentration for recovery of adsorbed ions were investigated. At pH 5, the maximum adsorption was obtained. At optimal pH value, adsorption capacity of sorbent for metal was obtained as 1,81 mg.g(-1), when initial concentration was 60 mg.ml(-1). Influence of HCl, NaCl, NH4Cl and EDTA solutions on desorption were investigated and maximum recovery was achieved by 0,2M EDTA solution. Desorption were 98% level provided by 0,2 M EDTA solution.
  • [ X ]
    Öğe
    The effects of Ara-C, simvastatin and combo therapy on energy metabolism of HL-60 promyolocytic leukemia cell lines
    (Turkish Biochemistry Society, 2013) Boyuna?a, Hakan; Günnur Dikmen, Z.; Kenar, Levent; Saygun, Oral; Keleş, Hatice; Ugur Ural, Ali; Lale, Mustafa
    Objective: Cancer cells choose their metabolic pathway depending on the oxygen content and substrate concentration of the medium. A wide spectrum of therapeutic agents regulating the energy metabolism of cancer cells are in still in use. Cytosine arabinoside (Ara-C) is a pyrimidine analogue used in the treatment of acute myeloid leukemia (AML) and simvastatin is an inhibitor of HMG-CoA (3-hydroxy-3-methyl-glutaryl-CoA) reductase, which regulates cholesterol biosynthesis. Thus, this study aimed to assess the energy metabolism of HL-60 promyelocytic leukemia cells and healthy white blood cells, additionally to determine the effects of simvastatin and Ara-C, alone or in combination on the energy metabolism of these cells. Materials and Methods: Healthy white blood cells, untreated and treated HL-60 promyolocytic leukemia cell lines were incubated for 4 hours with radiolabelled glucose. Following incubation, lactate, which is one of the end products of the carbohydrate catabolism, and radiolabelled CO2 produced by cells were collected and measured by the liquid scintillation device. In addition, glycogen consumption per hour was determined in each group. Results and Conclusion: We found that untreated HL-60 promyolocytic cells use anaerobic glycolytic pathway whereas healthy white blood cells use aerobic glycolysis for energy gain. It was concluded that combined use of Ara-C and Simvastatin might lead to significant increase in the rate of aerobic glycolysis of HL-60 promyelocytic cells and the metabolism of these leukemia cells become more similar to the metabolism of healthy white blood cells which they originate from. © TurkJBiochem.com.