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Öğe Oxidative stress in akkaraman ewes with seropositive for schmallenberg Virus(Ataturk Universitesi, 2018) Macun H.C.; Çinar M.; Azkur A.K.; Kalender H.; Erat S.The aim of this study was to determine oxidant-antioxidant status by evaluating some oxidative stress parameters (nitric oxide (NO), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), catalase activity) in clinically healthy Akkaraman ewes with seropositive for Schmallenberg virus (SBV). The study consisted of four positive, six suspected and seven negative-control blood sera in terms of specific antibody for SBV by using commercial ELISA kits. Even though there were no significant statistical differences (P>0.05), the highest NO level was in sera with SBV specific antibody positive while the lowest TOS level was in sera with SBV specific antibody negative. The values of TAS in sera were very similar in the groups (P=0.981). While the MDA levels of sera with SBV specific antibody positive and suspected were similar, the statistical difference was found between the MDA level of sera with SBV specific antibody positive and negative (P=0.021). The catalase activity of sera with SBV specific antibody positive was lower (P=0.030) than the catalase activity of sera with SBV suspected whereas the catalase activities in positive and negative groups and the catalase activities in suspected and negative groups were similar. In conclusion, it was determined that MDA level was affected in Akkaraman ewes that are SBV specific antibody positive without clinical symptoms. © 2018 Ataturk Universitesi.All Rights Reserved.Öğe Seroprevalance and co-existence of brucellosis, listeriosis and toxoplasmosis in dairy cattle in Kırıkkale province(Veteriner Fakultesi Dergisi, 2008) Öcal N.; Babür C.; Yağci B.B.; Macun H.C.; Çelebi B.; Kiliç S.; Pir Yağci I.This study was conducted on a total of 100 dairy cows of Holstein and Holstein crossbred cows of 3-7 years of age between December 2005 and April 2006. The aim of the study was to determine seroprevalence of brucellosis, listeriosis and toxoplasmosis, and the frequency of their co-existence in dairy cows. Clinically, the mean pulsus rate, breathing rate and body temperature were determined as 72±12/min., 24±6/min and 38.2±0.6°C, respectively. Antibodies against Brucella abortus were determined by means of Microplate Agglutination Test (MAT). The titres above 1/40 were considered seropositive. The 'O' antibodies, generated against Listeria monocytogenes, were determined by Osebold method. At least 2 (+) results at the titres above 1/100 were considered seropositive. Toxoplasma gondii antibodies were analyzed by Sabin-Feldman Dye Test (SFBT). The titres above 1/16 were considered seropositive. As a result, in dairy cows of Kirikkale region the seroprevalence of brucellosis, listeriosis and toxoplasmosis were 19%, 37% and 53%, respectively. The frequency of co-existence all three together was 5%. The frequency of co-existence of brucella with listeria was 4%, of brucella with toxoplasma was 7%, and of listeria with toxoplasma was 13%.