Yazar "Melekoglu, Abdullah" seçeneğine göre listele

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    Comparative cheliceral morphology of the solifuge species Biton zederbaueri (Werner) and Gluviopsilla discolor (Kraepelin) through scanning electron microscopy (Arachnida: Solifugae: Daesiidae)
    (Max Kasparek Verlag, 2011) Bayram, Abdullah; Yigit, Nazife; Erdek, Melek; Koc, Halil; Sancak, Zafer; Melekoglu, Abdullah; Ocal, Ilkay Corak
    A detailed comparative morphology of the male chelicerae of Biton zederbaueri (Werner) and Gluviopsilla discolor (Kraepelin) is presented using stereo microscope and scanning electron microscopy (SEM). The morphology of the cheliceral teeth, setae, flagella and some other organs such as the stridulatory organ that are used for description and identification are described and illustrated. The flagella are membranous, but their shapes arc different in both species. The stridulatory organs are similar in both daesiids, being reniform and with seven ridges. On the chelicerae, numerous long-thin, short-thin and short-thick setae are characteristic for both species.
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    Effects of Thalidomide and rhGM-CSF on Carbohydrate Metabolism in HL-60 Acute Promyelocytic Leukemia Cell Line
    (Akad Doktorlar Yayinevi, 2011) Boyunaga, Hakan; Dikmen, Gunnur; Ural, A. Ugur; Melekoglu, Abdullah
    The purpose of this study is to investigate the in vitro effect of thalidomide and rhGM-CSF on energy metabolism rate in HL-60 cell lines. HL-60 cells were incubated with radiolabelled glucose for 4 hours. Following incubation, radiolabelled CO2 was isolated and collected in specially designed scintillation vials. The other end-products of carbohydrate catabolism collected via anion-exchange chromatography were analyzed using liquid scintillation. Protein and glycogen levels for each group were also determined. The study results indicated that the use of drugs for 2-days had no significant effect when compared with aerobic and anaerobic controls. However, groups treated with the drugs for 5 days showed significant differences. It was obvious that rhGM-CSF caused HL-60 leukemia cells to use aerobic glycolytic pathway for production of energy. When thalidomide and rhGM-CSF combination therapy results were compared with untreated control cells, it was observed that glycogen consumption was decreased by 50.37%, and CO2 production was increased by 94.03%. When compared with those of anaerobic controls, glycogen consumption and CO2 production rates were found to be decreased by 54.01% and increased by 96.59%, respectively. As a consequence, we found that those cell lines treated with combined use of Thalidomide and rhGM-CSF caused HL-60 leukemia cells to predominantly prefer aerobic glycolytic pathways for energy production.
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    Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line
    (ANKARA UNIV PRESS, 2020) Melekoglu, Abdullah; Ekici, Husamettin; Arat, Esra; Karahan, Siyami
    Melamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 mu g/mL, 500 mu g/mL, 250 mu g/mL, 125 mu g/mL and 62.5 mu g/mL). At the highest concentration (1000 mu g/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.
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    Morphological Comparison of the Malleoli (Racquet Organs) in Biton zederbaueri and Gluviopsilla Discolor (Daesiidae, Solifugae)
    (Amer Entomol Soc, 2012) Yigit, Nazife; Erdek, Melek; Koc, Halil; Melekoglu, Abdullah
    The detailed morphology of the malleoli in two male solfugids, Biton zederbaueri (Werner, 1905) and Gluviopsilla discolor (Kraepelin, 1899), (Daesiidae, Solifugae) arc described and illustrated. This study was performed using stereo and scanning electron microscopy (SEM). Solifuges have five pairs of malleoli on the ventral surface of the fourth pair of legs of both sexes, as distinct from other arachnids. Malleolar sensory systems function as mechanoreceptors and chemoreceptors. A malleolus consists of two parts: a basal stalk and a triangular fan. There are epicuticular protrusions on the anterior face of each fan, and there are granular/vesicular structures on each stalk. Also, there are waved surfaces on the distal of the fan. This is the first study on the morphology of malleoli in solifuges in Turkey.