Yazar "Miraloglu, Ibrahim Halil" seçeneğine göre listele

Listeleniyor 1 - 1 / 1
  • [ X ]
    Öğe
    Comprehensive genetic analysis of the first near-completegenome of bovine coronavirus and partial genome ofbovine rotavirus in Türkiye through metagenomicsEmel
    (Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M, 2024) Aksoy, Emel; Azkur, Ahmet Kurat; Miraloglu, Ibrahim Halil
    Obtaining the complete or near-complete genome sequence of pathogens is becoming increasingly crucial forepidemiology, virology, clinical science and practice. This study aimed to detect viruses and conduct geneticcharacterization of genomes using metagenomics in order to identify the viral agents responsible for a calf's diarrhoea.The findings showed that bovine coronavirus (BCoV) and bovine rotavirus (BRV) are the primary viral agentsresponsible for the calf's diarrhoea. The current study successfully obtained the first-ever near-complete genomesequence of a bovine coronavirus (BCoV) from T & uuml;rkiye. The G+C content was 36.31% and the genetic analysisrevealed that the Turkish BCoV strain is closely related to respiratory BCoV strains from France and Ireland, with highnucleotide sequence and amino acid identity and similarity. In the present study, analysis of the S protein of theTurkish BCoV strain revealed the presence of 13 amino acid insertions, one of which was found to be shared with theFrench respiratory BCoV. The study also identified a BRV strain through metagenomic analysis and detected multiplemutations within the structural and non-structural proteins of the BRV strain, suggesting that the BRV Kirikkale strainmay serve as an ancestor for reassortants with interspecies transmission, especially involving rotaviruses that infectrabbits and giraffes