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Öğe Caries prevalence, oral health practices/behaviours and dental anxiety levels amongst dental hygiene students: A cross-sectional study(Wiley, 2022) Mutluay, Merve; Mutluay, Abidin TalhaObjective This study aimed to determine caries prevalence, oral health practices/behaviours and dental anxiety levels amongst dental hygiene students. Materials and Methods A self-administered questionnaire was used to determine students' oral hygiene practices/behaviours. The students were subjected to oral examinations to determine their decayed, missing, filled teeth (DMFT) scores according to the WHO criteria. In addition, the Corah's Dental Anxiety Scale (CDAS) was used to measure the students' dental anxiety levels. Results A total of 280 students (mean age: 20.8 years) participated in this study. Amongst the students, 52.8% brush their teeth twice a day, and 17.48% and 15.17% use floss and mouthwash daily, respectively. Moreover, the defined smoking rate was 22.85%. The mean DMFT scores of the preclinical and clinical students were 3.39 +/- 3.22 and 3.55 +/- 3.19, respectively. Most of the students (82.6%) preferred fluoride toothpaste, and the DMFT scores of those who use and do not use fluoride toothpaste significantly differed (p = 0.031). Furthermore, junk-food consumption affected the scores for decayed teeth (p = 0.048) and missing teeth (p = 0.039). Most students had low or moderate CDAS scores (CDAS <13), and anxiety scores were lower amongst the clinical students than amongst the preclinical students. Conclusions Although the students demonstrated favourable oral health behaviours/practices and low caries prevalence, their adherence to oral hygiene instructions and to healthy dietary and lifestyle habits should improve. The importance of this topic should be strongly emphasized in the undergraduate curriculum. For this purpose, professional behavioural control methods that suit students' individual capacities, motivations and therapeutic needs may be employed. The principal findings of similar studies should be evaluated by lecturers to help students adopt optimal oral health practices/behaviours, reducing their dental anxiety levels and improving their professional competencies.Öğe Characterisation of the calcium silicate-based cement-composite interface and the bonding strength with total-etch or single/two-stage self-etch adhesive systems(Wiley, 2022) Mutluay, Abidin Talha; Mutluay, MerveDue to favourable biological and physical properties, calcium silicate-based cements (CSCs) are biocompatible materials used widely for vital pulp therapies. Sealing efficacy between the adhesive system and CSC determines the clinical success of treatment. This study aimed to evaluate the shear bond strength (SBS) of CSC to composite resin with different adhesive systems and to analyse the CSC-composite interface. Mineral trioxide aggregate (MTA) (ProRoot MTA; Dentsply Tulsa Dental, Tulsa, OK), Biodentine (Septodont, Saint-Maur-des-Fosses, France) and NeoMTA Plus (Avalon Biomed Inc., Bradenton, FL) samples (n = 90) were placed in the cavities and divided into three subgroups according to the adhesive family (n = 10). SBSs were measured using a universal testing machine. The interfaces were examined using a scanning electron microscope (SEM) (x1000). NeoMTA Plus showed significantly higher SBS values than MTA and Biodentine (P < 0.0001). The application of total-etch adhesive system over Biodentine and NeoMTA Plus provided the statistically highest bond strength (P < 0.05). However, when the SBS values of MTA subgroups were compared, different adhesive families applied over MTA did not make a significant difference in SBS values (P > 0.05). Based on SEM analyses, the specimens exhibit no gaps, cracks or delamination within the adhesive layer, which indicates a good adhesion between the CSC-composite interface in all subgroups. In conclusion, the different adhesive families used over CSC did not influence interfacial gap formation.Öğe Characterisation of the calcium silicate-based cement–composite interface and the bonding strength with total-etch or single/two-stage self-etch adhesive systems(John Wiley and Sons Inc, 2022) Mutluay, Abidin Talha; Mutluay, MerveDue to favourable biological and physical properties, calcium silicate-based cements (CSCs) are biocompatible materials used widely for vital pulp therapies. Sealing efficacy between the adhesive system and CSC determines the clinical success of treatment. This study aimed to evaluate the shear bond strength (SBS) of CSC to composite resin with different adhesive systems and to analyse the CSC–composite interface. Mineral trioxide aggregate (MTA) (ProRoot MTA; Dentsply Tulsa Dental, Tulsa, OK), Biodentine (Septodont, Saint-Maur-des-Fossés, France) and NeoMTA Plus (Avalon Biomed Inc., Bradenton, FL) samples (n = 90) were placed in the cavities and divided into three subgroups according to the adhesive family (n = 10). SBSs were measured using a universal testing machine. The interfaces were examined using a scanning electron microscope (SEM) (×1000). NeoMTA Plus showed significantly higher SBS values than MTA and Biodentine (P < 0.0001). The application of total-etch adhesive system over Biodentine and NeoMTA Plus provided the statistically highest bond strength (P < 0.05). However, when the SBS values of MTA subgroups were compared, different adhesive families applied over MTA did not make a significant difference in SBS values (P > 0.05). Based on SEM analyses, the specimens exhibit no gaps, cracks or delamination within the adhesive layer, which indicates a good adhesion between the CSC–composite interface in all subgroups. In conclusion, the different adhesive families used over CSC did not influence interfacial gap formation. © 2021 Australian Society of Endodontology Inc.Öğe Does Achievement of Hemostasis After Pulp Exposure Provide an Accurate Assessment of Pulp Inflammation?(Amer Acad Pediatric Dentistry, 2018) Mutluay, Merve; Arikan, Volkan; Sari, Saziye; Kisa, UclerPurpose: The purpose of this study was to determine in primary molars with carious exposures whether hemostasis at the exposure site and pulp orifice reflected inflammatory status of the pulp at the canal orifice based on cytokine levels. Methods: Forty mandibular primary molars with deep caries were included in the study. Teeth were divided into two groups: group A had teeth where hemostasis at the exposure site was achieved within five minutes, and group B had teeth where hemostasis at the exposure site could not be achieved within five minutes. Blood samples were harvested from the exposure sites and canal orifices. Cytokine levels for IL-1 beta, IL-2, IL-6, IL-8, IL-10, TNF-alpha, and PGE(2) were measured using ELISA for all sample sites. Results: The IL-6 levels at the exposure sites were found to be significantly higher in group A when compared to group B, but there was no statistically significant differences in any of the cytokine levels at the canal orifices between the two groups. Conclusions: Controlling bleeding at the exposure site or canal orifices does not provide accurate assessment of inflammation at the canal orifice and may be misleading for diagnosing vital pulp treatment in primary teeth with a carious pulp exposure.Öğe Educational concerns and awareness level among dental hygiene students during the COVID-19 pandemic(Wiley, 2022) Mutluay, Abidin Talha; Mutluay, MerveObjective This study aimed to evaluate the educational concerns and awareness level among dental hygiene students during the COVID-19 pandemic. Materials and Methods A questionnaire, created via Google Forms, was sent electronically to 240 dental hygiene students from Turkey, of which 223 replied to the survey. The questionnaire was divided into 4 parts and consisted of 24 close-ended questions. Results The main symptoms of COVID-19 were correctly recognized by 71% of the students, and 40.2% of the students were aware of the transmission routes. Of all, 77.1% of the students expressed fear of getting infected, 96.9% believed that they would more likely get infected while performing dental procedures, and 91% believed that dental hygienists' cross-contamination risk was high. Furthermore, 69.5% of the students stated that their undergraduate education process was negatively affected, 59.6% appreciated ongoing online education, and 31.8% wanted face-to-face clinical internships. Conclusions Although most of the students were aware of the transmission routes and preventive measures of COVID-19, concerns regarding disease transmission among them remain. Students appreciated the ongoing online education and believed that face-to-face education is risky during this current pandemic. However, they believe that this process can adversely affect their professional knowledge and especially practical skills. A balance must be struck between sustaining an effective dental hygiene education process and containing the spread of COVID-19.Öğe Effect of work environment and specialty degree of dentists on cross-infection control in COVID-19 pandemic(Elsevier Brazil, 2021) Mutluay, Merve; Egil, EdibeObjectives: The aim of this study was to evaluate the effect of the work environment and expertise/specialty degree of dentists on their behavior, awareness, and attitudes regarding cross-infection control during the COVID-19 pandemic. Design: The study population consisted of Turkish dentists who work in private clinics, public clinics and university hospitals. The demographic information of the participants, their awareness of the COVID-19 acute respiratory disease, and clinical measures taken against cross-infection were evaluated with an online survey. Between the 10th and 20th of November 2020, 2,400 surveys were e-mailed to dentists. Results: A total 454 professionals answered the survey. According to the results, 29.3% of the participants performed only urgent care during the pandemic period, whereas 59.9% of them performed both urgent and routine treatments. Among the responding dentists, 90.6% stated that they were worried about aerosol-generating dental procedures, but there was no differences between genders (p = 0.119). Most participants, especially specialists (p = 0.160) , applied strict cross-infection control methods during the COVID19 pandemic (77.2%). These dentists used personal protective equipment (PPE) at rates that varied between 75.5% and 98.4%. Nonetheless, the rate of PPE use was different between genders and degrees of expertise: women used PPE more frequently than men (p = 0.025), and specialists used PPE more often than the other dentists (p = 0.04). Finally, there was a weak positive correlation between the level of PPE use and expertise (r = 0.121; p = 0.010). Conclusions: Despite the overall knowledge of the participants regarding COVID-19 symptoms, transmission routes, and the guidelines needed to prevent the virus from spreading, the dental specialists followed infection control methods more strictly. Even though the participants were concerned about dental practices that create microbial aerosols during the pandemic period, they continued their clinical routines using high PPE levels and taking extra clinical precautions to avoid cross-infection. (C) 2021 Sociedade Brasileira de Infectologia. Published by Elsevier Espana, S.L.U.Öğe Evaluation of Salivary Thiol/Disulfide Homeostasis and Oxidative Stress in Children with Severe Early Childhood Caries Using a Novel Method(Georg Thieme Verlag Kg, 2022) Onsuren, Asli Sogukpinar; Mutluay, Merve; Seyithanoglu, Muhammed; Tanriverdi, BurakObjective This study aimed to assess the role of thiol/disulfide homeostasis and oxidative stress in the saliva of children with severe early childhood caries (S-ECC). Methods Eighty children aged 3 to 6 years were involved in this case-control study. The study consisted of two groups: the study group (S-ECC) and the control group with no caries. Thiol/disulfide homeostasis and antioxidant levels were calculated after obtaining unstimulated saliva samples from all participating children. Results The native/total thiol and total oxidant status (TOS) levels of the study group were higher than those of the control group, though not statistically significant ( p > 0.05). The oxidative stress index (OSI) value was significantly higher in the study group compared to the control group ( p = 0.024). Conclusion Our results confirmed that the thiol/disulfide homeostasis was reduced, and disulfide formation, which is rereducible to thiol, was insufficient in children with S-ECC to compensate oxidative stress compared with the control group. Also, thiol levels were inadequate to compensate for oxidative stress, and thiol/disulfide homeostasis was not an independent parameter for S-ECC. Besides, the increases in the TOS level and OSI value show that oxidative stress had significant effects on S-ECC's etiopathogenesis.Öğe Evaluation of the effectiveness of different irrigation agents in the removal of calcium hydroxide from root canals(AVES, 2022) Mutluay, MerveObjective: Calcium hydroxide [Ca(OH)2 ], is the most commonly used intracanal medicament in endodontic treatments. It has antimicrobial activity against endodontic pathogens. This study aimed to compare the efficiency of ethylenediaminetetraacetic acid, citric acid, maleic acid, and EDTA+setrimid combination in the removal of Ca(OH)2 intracanal medicament from root canals with manual irrigation using a syringe. Methods: A total of 72 single-rooted bovine incisors were prepared, and Ca(OH)2 paste was placed in the working length with lentulo to the root canals for the study. Seven days later, samples were established to 6 groups according to the Ca(OH)2 removal techniques: 17% EDTA, 10% citric acid, 7% maleic acid, 17% ethylenediaminetetraacetic acid +0.2% cetrimide combination, positive control group [no Ca(OH)2 paste], and negative control group (no irrigation). Residual Ca(OH)2 was scored using a 4-grade scoring system, and the data were analyzed statistically using the Kruskal–Wallis test (P =.05). Results: Ethylenediaminetetraacetic acid +cetrimide combination showed the best results regarding calcium hydroxide removal from the coronal part. In the apical part, while ethylenediaminetetraacetic acid +cetrimide’s efficiency was higher than malic acid (P =.020), no statistically significant difference was observed between ethylenediaminetetraacetic acid and citric acid’s efficiency (P >.05). Conclusion: There is no difference in terms of Ca(OH)2 removing ability of ethylenediaminetetraacetic acid, citric acid, and malic acid from root canals. Since cetrimide addition has been shown to improve ethylenediaminetetraacetic acid’s Ca(OH)2 removing ability from root canals, cetrimide irrigation can be recommended to removal of Ca(OH)2. © 2022, AVES. All rights reserved.