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    The activities of GST isozymes in stomach tissues of female obese patients
    (De Gruyter Open Ltd, 2020) Yilmaz C.; Bulus H.; Oguztuzun S.; Cihan M.; Fidan C.
    Objectives: Obesity has become an important public health problem because of its increasing prevalence and relation with many diseases and mortality. Studies have shown its association with oxidative stress. In this study, the effect of obesity on total amount of thiol and some glutathione S-transferase (GST) isozymes were investigated which could serve as an important criteria in dose adjustment of some certain drugs in obese. Methods: The gastric tissues removed by gastrectomy operation from 29 morbid obese female patients were analysed for thiol levels and activities of total GST, GSTT1-1 and GSTM1-1. Patients were grouped according to age, presence of hypertension and/or diabetes, and family history. Results: The average total thiol was 131.22 (±7.74) nmol/ mg protein with no significant differences in between the groups. GSTT1 specific activities were about 20% higher in four groups: with ages over 35 years old, with hypertension, without diabetes and finally without family history, with respect to other groups. The differences between total GST and GSTM1 activity levels of experimental groups were not significant. Conclusions: This is the first study to compare activities of GST isozymes and total thiol content in the stomach tissues of obese female patients accompanying some common metabolic disorders, age and family history. © 2020 De Gruyter. All rights reserved.
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    New pathway in rheumatic mitral valve disease: Cytochrome P450 and glutathione S transferase isozyme expression
    (De Gruyter Open Ltd, 2020) Simsek E.; Simsek G.; Soyal M.F.T.; Kaygin P.; Oguztuzun S.; Duzgun A.C.; Sarialtin S.
    Objective: Cytochrome P450 (CYP)1A1, glutathione S-transferase pi (GSTP1) and omega (GSTO1) isozymes were evaluated and compared in patients with the diagnosis of rheumatic mitral valve disease and ischemic mitral valve insufficiency to find out the relationship of the oxidative stress with rheumatic mitral valve disease. Materials and methods: The control group consisted of patients operated on due to ischemic mitral valve insufficiency (group I, n:14) while study group consisted of the patients operated on with the diagnosis of rheumatic mitral valve disease (group II, n:29). Mitral valve materials were stained with hematoxylin and eosin. CYP1A1, GSTP1, and GSTO1 immunohistochemical markers were studied. Results: 20.7% of GSTP1 isozyme protein expression was seen in the study group; however, no expression was detected in the control group. This finding was statistically significant in terms of GSTP1 isozyme. No statistically significant differences in the level of GSTO1 and CYP1A1 protein expression between the study and control groups were observed. Conclusion: In this study, we found out that GSTP1 isozyme may be related to rheumatic mitral valve disease. A strategy that would help prevent oxidative stress in patients with rheumatic mitral valve disease can be a so valuable means to affect disease progression. © 2020 De Gruyter. All rights reserved.
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    Prognostic significance of caspase- 3, Bcl-2, P53 and GSTPI expressions in lung adenocarcinoma
    (Society of Pharmaceutical Sciences of Ankara (FABAD), 2013) Oguztuzun S.; Oguz Ada A.; Kilic M.; Cakir E.; Yilmaz A.
    Lung adenocarcinoma is one of the most fatal cancers in humans, and many factors are known to contribute to its poor prognosis. The aim of this work is to investigate the prognostic value of the apoptosis-regulating markers (caspase-3, bcl-2, p53) and the important detoxification enzyme (GST pi), and analyze the relationships among these immunohistochemical (IHC) results, patients' clinical characteristics, and survival. IHC stainings were performed on 89 lung adenocarcinoma specimens obtained from 89 patients from the Atatürk Chest Diseases and Thoracic Surgery Training and Research Hospital, Ankara, Turkey. The medical records of the patients were reviewed retrospectively. The correlation between the presence of protein expression and clinicopathological parameters (gender, age, tumor stage and histology) was examined by using the Pearson correlation test. Survival analysis was carried out by the Kaplan-Meier method. The prevalence of caspase 3, bcl-2, p53, and GST pi expression was 48%, 57%, 95% and 52%, respectively. The expression rate of p53 was relatively high in smokers than that in non-smokers. There were no correlations among the immunoreactivities of bcl- 2 and GST pi in clinical stage, and overall survival. However, there was a significant relationship between caspase-3 and p53 expressions and overall survival. Caspase-3 expression was correlated with good prognosis, and p53 expression was correlated with poor prognosis.